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Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus

Percentage of viable Puccinia striiformis f. sp. tritici (Pst) quantified by qPCR of RNA in detached (A) and attached (B) leaves of cv. Mingxian 169 after incubation at different temperatures for a varying length of time.The experiment was done three times. The vertical bar of each mean value represents the standard deviation of the three mean values from the three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. The temperature treatments with different letters at the same sampling time differed significantly at P = 0.05. There were no significant (P > 0.05) differences between -15°C and -20°C at the sampling time of 18, 24 and 30 h.
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pone.0130691.g006: Percentage of viable Puccinia striiformis f. sp. tritici (Pst) quantified by qPCR of RNA in detached (A) and attached (B) leaves of cv. Mingxian 169 after incubation at different temperatures for a varying length of time.The experiment was done three times. The vertical bar of each mean value represents the standard deviation of the three mean values from the three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. The temperature treatments with different letters at the same sampling time differed significantly at P = 0.05. There were no significant (P > 0.05) differences between -15°C and -20°C at the sampling time of 18, 24 and 30 h.

Mentions: Overall, the percentage of Pst that survived in detached and attached leaves decreased (P < 0.001) over time at all test temperatures (Fig 6A–6B). Pst survival differed greatly (P < 0.001) between attached and detached leaves. A greater (P < 0.001) percentage of Pst survived in attached leaves than in detached leaves after prolonged incubation: at 0°C during 42–54 h, at -5°C during 42–48 h, at -10°C during 36–42 h and at -15°C and -20°C after 18 h. This difference was most pronounced at 0°C (Fig 6B). Viable Pst was still detected after incubation at 0°C and -5°C for 54 h in both detached and attached leaves. The percentage of Pst that survived was 10.1% and 4.0% at 0°C and -5°C, respectively after incubation for 54 h in detached leaves; the corresponding values in attached leaves were 25.8% and 5.5%, respectively. The amount of viable Pst was below the limit of detection after incubation at -10°C for 48 h, at -15°C for 30 h in both attached and detached leaves and at -20°C for 24 h in detached leaves and for 30 h in attached leaves. Plants that were incubated at -20°C for 30 h, -15°C for 30 h, -10°C for 42 h, -5°C and 0°C for 54 h were still alive when transferred into 4°C for 1 d and then maintained under normal conditions in a greenhouse for one week.


Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Percentage of viable Puccinia striiformis f. sp. tritici (Pst) quantified by qPCR of RNA in detached (A) and attached (B) leaves of cv. Mingxian 169 after incubation at different temperatures for a varying length of time.The experiment was done three times. The vertical bar of each mean value represents the standard deviation of the three mean values from the three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. The temperature treatments with different letters at the same sampling time differed significantly at P = 0.05. There were no significant (P > 0.05) differences between -15°C and -20°C at the sampling time of 18, 24 and 30 h.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470655&req=5

pone.0130691.g006: Percentage of viable Puccinia striiformis f. sp. tritici (Pst) quantified by qPCR of RNA in detached (A) and attached (B) leaves of cv. Mingxian 169 after incubation at different temperatures for a varying length of time.The experiment was done three times. The vertical bar of each mean value represents the standard deviation of the three mean values from the three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. The temperature treatments with different letters at the same sampling time differed significantly at P = 0.05. There were no significant (P > 0.05) differences between -15°C and -20°C at the sampling time of 18, 24 and 30 h.
Mentions: Overall, the percentage of Pst that survived in detached and attached leaves decreased (P < 0.001) over time at all test temperatures (Fig 6A–6B). Pst survival differed greatly (P < 0.001) between attached and detached leaves. A greater (P < 0.001) percentage of Pst survived in attached leaves than in detached leaves after prolonged incubation: at 0°C during 42–54 h, at -5°C during 42–48 h, at -10°C during 36–42 h and at -15°C and -20°C after 18 h. This difference was most pronounced at 0°C (Fig 6B). Viable Pst was still detected after incubation at 0°C and -5°C for 54 h in both detached and attached leaves. The percentage of Pst that survived was 10.1% and 4.0% at 0°C and -5°C, respectively after incubation for 54 h in detached leaves; the corresponding values in attached leaves were 25.8% and 5.5%, respectively. The amount of viable Pst was below the limit of detection after incubation at -10°C for 48 h, at -15°C for 30 h in both attached and detached leaves and at -20°C for 24 h in detached leaves and for 30 h in attached leaves. Plants that were incubated at -20°C for 30 h, -15°C for 30 h, -10°C for 42 h, -5°C and 0°C for 54 h were still alive when transferred into 4°C for 1 d and then maintained under normal conditions in a greenhouse for one week.

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus