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Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus

Estimated Puccinia striiformis f. sp. tritici of 5 mg urediniospores at three storage conditions using the qPCR of DNA (dashed lines) and RNA (solid lines).Dashed lines with triangles, squares and circles represent DNA from dead urediniospores at ambient temperature, fresh urediniospores at ambient temperature and at -20°C, respectively. Solid lines with triangles, squares and circles represent RNA from dead urediniospores at ambient temperature, and fresh urediniospores at ambient temperature and at -20°C, respectively. The experiment was done three times. The vertical bar of each treatment at each time point represents the standard deviation of the three mean values from three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. Treatments with different letters for the 12-days means were significantly different at P = 0.05. There were no significant differences among the treatments on 0, 4 and 8 days at P = 0.05.
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pone.0130691.g004: Estimated Puccinia striiformis f. sp. tritici of 5 mg urediniospores at three storage conditions using the qPCR of DNA (dashed lines) and RNA (solid lines).Dashed lines with triangles, squares and circles represent DNA from dead urediniospores at ambient temperature, fresh urediniospores at ambient temperature and at -20°C, respectively. Solid lines with triangles, squares and circles represent RNA from dead urediniospores at ambient temperature, and fresh urediniospores at ambient temperature and at -20°C, respectively. The experiment was done three times. The vertical bar of each treatment at each time point represents the standard deviation of the three mean values from three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. Treatments with different letters for the 12-days means were significantly different at P = 0.05. There were no significant differences among the treatments on 0, 4 and 8 days at P = 0.05.

Mentions: The amplified fragment size was 248 bp and 159 bp from DNA and cDNA of Pst using EF1 primer pair, respectively (S2 Fig). Standard curves based on RNA (cDNA) and DNA were directly estimated using software iQ5 (Fig 3); the coefficient of determination (R2) for standard curves was greater than 0.98 in all quantification runs. Amplification efficiency ranged from 95.1% to 101.2% for RNA (cDNA) and from 97.3% to 99.7% for DNA. Five mg of Pst urediniospores under three storage conditions for 0–12 days were assayed using the qPCR of DNA and RNA (cDNA) (Fig 4). The amount of quantified Pst significantly differed (P < 0.01) between the two methods [qPCR of DNA and RNA (cDNA)]. However, both methods could not differentiate dead, fresh and dormant urediniospores except for a much lower amount of quantified Pst by qPCR of RNA for dead urediniospores at the ambient temperature (Fig 4).


Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Estimated Puccinia striiformis f. sp. tritici of 5 mg urediniospores at three storage conditions using the qPCR of DNA (dashed lines) and RNA (solid lines).Dashed lines with triangles, squares and circles represent DNA from dead urediniospores at ambient temperature, fresh urediniospores at ambient temperature and at -20°C, respectively. Solid lines with triangles, squares and circles represent RNA from dead urediniospores at ambient temperature, and fresh urediniospores at ambient temperature and at -20°C, respectively. The experiment was done three times. The vertical bar of each treatment at each time point represents the standard deviation of the three mean values from three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. Treatments with different letters for the 12-days means were significantly different at P = 0.05. There were no significant differences among the treatments on 0, 4 and 8 days at P = 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470655&req=5

pone.0130691.g004: Estimated Puccinia striiformis f. sp. tritici of 5 mg urediniospores at three storage conditions using the qPCR of DNA (dashed lines) and RNA (solid lines).Dashed lines with triangles, squares and circles represent DNA from dead urediniospores at ambient temperature, fresh urediniospores at ambient temperature and at -20°C, respectively. Solid lines with triangles, squares and circles represent RNA from dead urediniospores at ambient temperature, and fresh urediniospores at ambient temperature and at -20°C, respectively. The experiment was done three times. The vertical bar of each treatment at each time point represents the standard deviation of the three mean values from three experiments; significant treatment differences were based on the pooled residual error in the repeated measurement ANOVA. Treatments with different letters for the 12-days means were significantly different at P = 0.05. There were no significant differences among the treatments on 0, 4 and 8 days at P = 0.05.
Mentions: The amplified fragment size was 248 bp and 159 bp from DNA and cDNA of Pst using EF1 primer pair, respectively (S2 Fig). Standard curves based on RNA (cDNA) and DNA were directly estimated using software iQ5 (Fig 3); the coefficient of determination (R2) for standard curves was greater than 0.98 in all quantification runs. Amplification efficiency ranged from 95.1% to 101.2% for RNA (cDNA) and from 97.3% to 99.7% for DNA. Five mg of Pst urediniospores under three storage conditions for 0–12 days were assayed using the qPCR of DNA and RNA (cDNA) (Fig 4). The amount of quantified Pst significantly differed (P < 0.01) between the two methods [qPCR of DNA and RNA (cDNA)]. However, both methods could not differentiate dead, fresh and dormant urediniospores except for a much lower amount of quantified Pst by qPCR of RNA for dead urediniospores at the ambient temperature (Fig 4).

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus