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Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic.

Svobodová Z, Skoková Habuštová O, Hutchison WD, Hussein HM, Sehnal F - PLoS ONE (2015)

Bottom Line: However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required.Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05).However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic; Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic.

ABSTRACT
Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize), confers resistance to corn rootworms (Diabrotica spp.) and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009-2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G) and two non-Bt reference hybrids (KIPOUS and PR38N86). Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017) and non-Bt (DK315) untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

No MeSH data available.


Related in: MedlinePlus

Redundancy analysis of the spatial and temporal distribution of plant dwelling arthropods.Horizontal line indicates years and sample date and vertical line the management regimes.
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pone.0130656.g002: Redundancy analysis of the spatial and temporal distribution of plant dwelling arthropods.Horizontal line indicates years and sample date and vertical line the management regimes.

Mentions: Confirmation of the linear character of arthropod community (DCA: length of gradient: 2.8) was followed by integrated analysis of ecological preferences of plant dwelling arthropods implemented using a partial RDA. From the percentage of explained variability it is clear that arthropods did not prefer or avoided any specific Bt or non-Bt management regime (Table 2). However, two separate clusters of centroids for management regimes in Fig 2 show that the abundance of plant dwelling insects was similar in the genetically related hybrids—MON88017 and DK315, untreated or treated with insecticide. The reference hybrids KIPOUS and PR38N86 were somewhat different from the Bt maize and its near-isogenic hybrid, but similar to each other. The total contribution of management regimes to the explained variability was small (Table 2). The cluster of centroids near the intersection of the ordination space (crossing of the first and second axis) confirms low explanatory power of management regimes. Many other factors which could influence arthropod distributions in the field were rejected as well (plot position within the field, plot disturbance by the presence of the drainage wells or hunting lookout). Most of the variability in arthropod distributions was explained by the sample date and year.


Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic.

Svobodová Z, Skoková Habuštová O, Hutchison WD, Hussein HM, Sehnal F - PLoS ONE (2015)

Redundancy analysis of the spatial and temporal distribution of plant dwelling arthropods.Horizontal line indicates years and sample date and vertical line the management regimes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470634&req=5

pone.0130656.g002: Redundancy analysis of the spatial and temporal distribution of plant dwelling arthropods.Horizontal line indicates years and sample date and vertical line the management regimes.
Mentions: Confirmation of the linear character of arthropod community (DCA: length of gradient: 2.8) was followed by integrated analysis of ecological preferences of plant dwelling arthropods implemented using a partial RDA. From the percentage of explained variability it is clear that arthropods did not prefer or avoided any specific Bt or non-Bt management regime (Table 2). However, two separate clusters of centroids for management regimes in Fig 2 show that the abundance of plant dwelling insects was similar in the genetically related hybrids—MON88017 and DK315, untreated or treated with insecticide. The reference hybrids KIPOUS and PR38N86 were somewhat different from the Bt maize and its near-isogenic hybrid, but similar to each other. The total contribution of management regimes to the explained variability was small (Table 2). The cluster of centroids near the intersection of the ordination space (crossing of the first and second axis) confirms low explanatory power of management regimes. Many other factors which could influence arthropod distributions in the field were rejected as well (plot position within the field, plot disturbance by the presence of the drainage wells or hunting lookout). Most of the variability in arthropod distributions was explained by the sample date and year.

Bottom Line: However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required.Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05).However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic; Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic.

ABSTRACT
Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize), confers resistance to corn rootworms (Diabrotica spp.) and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009-2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G) and two non-Bt reference hybrids (KIPOUS and PR38N86). Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017) and non-Bt (DK315) untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

No MeSH data available.


Related in: MedlinePlus