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Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic.

Svobodová Z, Skoková Habuštová O, Hutchison WD, Hussein HM, Sehnal F - PLoS ONE (2015)

Bottom Line: However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required.Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05).However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic; Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic.

ABSTRACT
Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize), confers resistance to corn rootworms (Diabrotica spp.) and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009-2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G) and two non-Bt reference hybrids (KIPOUS and PR38N86). Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017) and non-Bt (DK315) untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

No MeSH data available.


Related in: MedlinePlus

Seasonal fluctuations of common plant dwelling insects in the trial years.Number of individuals per sample date (50 plants per management regime). A: Western flower thrips (Frankliniella occidentalis). B: Aphids (Aphididae: Metopolophium dirhodum, Rhopalosiphum padi, Sitobion avenae). C: Orius spp. D: Green lacewings (Chrysopidae)
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pone.0130656.g001: Seasonal fluctuations of common plant dwelling insects in the trial years.Number of individuals per sample date (50 plants per management regime). A: Western flower thrips (Frankliniella occidentalis). B: Aphids (Aphididae: Metopolophium dirhodum, Rhopalosiphum padi, Sitobion avenae). C: Orius spp. D: Green lacewings (Chrysopidae)

Mentions: Nine different orders of arthropods were recorded on all maize hybrids, including Bt maize (Table 1, numbers of individuals per plant are provided in S1 Table). The plant-sucking western flower thrips (Frankliniella occidentalis) was common in all plots, with highest abundance at the BBCH 65 stage or before (Fig 1). Approximately 15, 25 and 19 thrips per plant were found in the midsummer in 2009, 2010 and 2011 respectively. The lowest abundance of F. occidentalis was on reference hybrid PR38N86, but the differences were not statistically significant (Table 1).


Risk Assessment of Genetically Engineered Maize Resistant to Diabrotica spp.: Influence on Above-Ground Arthropods in the Czech Republic.

Svobodová Z, Skoková Habuštová O, Hutchison WD, Hussein HM, Sehnal F - PLoS ONE (2015)

Seasonal fluctuations of common plant dwelling insects in the trial years.Number of individuals per sample date (50 plants per management regime). A: Western flower thrips (Frankliniella occidentalis). B: Aphids (Aphididae: Metopolophium dirhodum, Rhopalosiphum padi, Sitobion avenae). C: Orius spp. D: Green lacewings (Chrysopidae)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470634&req=5

pone.0130656.g001: Seasonal fluctuations of common plant dwelling insects in the trial years.Number of individuals per sample date (50 plants per management regime). A: Western flower thrips (Frankliniella occidentalis). B: Aphids (Aphididae: Metopolophium dirhodum, Rhopalosiphum padi, Sitobion avenae). C: Orius spp. D: Green lacewings (Chrysopidae)
Mentions: Nine different orders of arthropods were recorded on all maize hybrids, including Bt maize (Table 1, numbers of individuals per plant are provided in S1 Table). The plant-sucking western flower thrips (Frankliniella occidentalis) was common in all plots, with highest abundance at the BBCH 65 stage or before (Fig 1). Approximately 15, 25 and 19 thrips per plant were found in the midsummer in 2009, 2010 and 2011 respectively. The lowest abundance of F. occidentalis was on reference hybrid PR38N86, but the differences were not statistically significant (Table 1).

Bottom Line: However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required.Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05).However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic; Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic.

ABSTRACT
Transgenic maize MON88017, expressing the Cry3Bb1 toxin from Bacillus thuringiensis (Bt maize), confers resistance to corn rootworms (Diabrotica spp.) and provides tolerance to the herbicide glyphosate. However, prior to commercialization, substantial assessment of potential effects on non-target organisms within agroecosystems is required. The MON88017 event was therefore evaluated under field conditions in Southern Bohemia in 2009-2011, to detect possible impacts on the above-ground arthropod species. The study compared MON88017, its near-isogenic non-Bt hybrid DK315 (treated or not treated with the soil insecticide Dursban 10G) and two non-Bt reference hybrids (KIPOUS and PR38N86). Each hybrid was grown on five 0.5 ha plots distributed in a 14-ha field with a Latin square design. Semiquantitative ELISA was used to verify Cry3Bb1 toxin levels in the Bt maize. The species spectrum of non-target invertebrates changed during seasons and was affected by weather conditions. The thrips Frankliniella occidentalis was the most abundant species in all three successive years. The next most common species were aphids Rhopalosiphum padi and Metopolophium dirhodum. Frequently observed predators included Orius spp. and several species within the Coccinellidae. Throughout the three-year study, analysis of variance indicated some significant differences (P<0.05). Multivariate analysis showed that the abundance and diversity of plant dwelling insects was similar in maize with the same genetic background, for both Bt (MON88017) and non-Bt (DK315) untreated or insecticide treated. KIPOUS and PR38N86 showed some differences in species abundance relative to the Bt maize and its near-isogenic hybrid. However, the effect of management regime on arthropod community was insignificant and accounted only for a negligible portion of the variability.

No MeSH data available.


Related in: MedlinePlus