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Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Fluorescence image showing MG-63 cell growth on the scaffold material.At 3 h a very small number of cells adhered to the scaffold, but at 6 h and 12 h the number of adhered MG-63 cells increased substantially. At 1 d the cell nuclei began to show dispersed and uniform blue fluorescence. At 2 d the fluorescence intensity increased substantially, and at 3 d a granular fluorescence was observed due to increased cell density.
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pone.0128658.g011: Fluorescence image showing MG-63 cell growth on the scaffold material.At 3 h a very small number of cells adhered to the scaffold, but at 6 h and 12 h the number of adhered MG-63 cells increased substantially. At 1 d the cell nuclei began to show dispersed and uniform blue fluorescence. At 2 d the fluorescence intensity increased substantially, and at 3 d a granular fluorescence was observed due to increased cell density.

Mentions: Hoechst staining revealed the adhesion and proliferation of cells on the scaffold materials. At 3 h only a very small number of cells adhered to the scaffold, but at 6 and 12 h the adhesion rates of MG-63 cells increased. At 100X magnification we noted that after 1 d the cell nuclei began to show dispersed and relatively uniform blue fluorescence, and at 2 d the fluorescence intensity increased substantially. On day 3, granular fluorescence was observed due to increased cell density (Fig 11).


Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Fluorescence image showing MG-63 cell growth on the scaffold material.At 3 h a very small number of cells adhered to the scaffold, but at 6 h and 12 h the number of adhered MG-63 cells increased substantially. At 1 d the cell nuclei began to show dispersed and uniform blue fluorescence. At 2 d the fluorescence intensity increased substantially, and at 3 d a granular fluorescence was observed due to increased cell density.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470591&req=5

pone.0128658.g011: Fluorescence image showing MG-63 cell growth on the scaffold material.At 3 h a very small number of cells adhered to the scaffold, but at 6 h and 12 h the number of adhered MG-63 cells increased substantially. At 1 d the cell nuclei began to show dispersed and uniform blue fluorescence. At 2 d the fluorescence intensity increased substantially, and at 3 d a granular fluorescence was observed due to increased cell density.
Mentions: Hoechst staining revealed the adhesion and proliferation of cells on the scaffold materials. At 3 h only a very small number of cells adhered to the scaffold, but at 6 and 12 h the adhesion rates of MG-63 cells increased. At 100X magnification we noted that after 1 d the cell nuclei began to show dispersed and relatively uniform blue fluorescence, and at 2 d the fluorescence intensity increased substantially. On day 3, granular fluorescence was observed due to increased cell density (Fig 11).

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus