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Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Fluorescence image showing MG-63 cell adhesion to the scaffold material.At 12 h after inoculation, the cells uniformly adhered to the pore walls of the scaffold.
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pone.0128658.g009: Fluorescence image showing MG-63 cell adhesion to the scaffold material.At 12 h after inoculation, the cells uniformly adhered to the pore walls of the scaffold.

Mentions: Freshly seeded cells were transparent, spherical, and uniformly distributed on the scaffold. At 6 h after inoculation, they started to adhere to the walls of the scaffold material (Fig 8). At 12 h, a large number of cells adhered to the scaffold (Fig 9). After 24 h, the majority of cells had completed adhesion and cell volumes increased; a small number of cells showed growth projections. Two days after inoculation, the cells became spindle-shaped or triangular with notable projections, and junctions between the growth projections began to form. At 3 d after inoculation, the cells were tightly connected to each other, and the walls and pores of the scaffold material were completely covered with cells.


Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Fluorescence image showing MG-63 cell adhesion to the scaffold material.At 12 h after inoculation, the cells uniformly adhered to the pore walls of the scaffold.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470591&req=5

pone.0128658.g009: Fluorescence image showing MG-63 cell adhesion to the scaffold material.At 12 h after inoculation, the cells uniformly adhered to the pore walls of the scaffold.
Mentions: Freshly seeded cells were transparent, spherical, and uniformly distributed on the scaffold. At 6 h after inoculation, they started to adhere to the walls of the scaffold material (Fig 8). At 12 h, a large number of cells adhered to the scaffold (Fig 9). After 24 h, the majority of cells had completed adhesion and cell volumes increased; a small number of cells showed growth projections. Two days after inoculation, the cells became spindle-shaped or triangular with notable projections, and junctions between the growth projections began to form. At 3 d after inoculation, the cells were tightly connected to each other, and the walls and pores of the scaffold material were completely covered with cells.

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus