Limits...
Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Change in the pH of the lysate and the degradation rate in the 40% SF-60% CS group.(A) pH fluctuated in a small range of 7.42~7.62; (B)The scaffold lost only 18.25% of its original weight after 64 days.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4470591&req=5

pone.0128658.g007: Change in the pH of the lysate and the degradation rate in the 40% SF-60% CS group.(A) pH fluctuated in a small range of 7.42~7.62; (B)The scaffold lost only 18.25% of its original weight after 64 days.

Mentions: During the degradation process that lasted for two months, the pH fluctuated in a small range 7.42–7.62 (Fig 7A). In the first 12 days, the pH slowly increased, possibly due to the gradual degradation of the alkaline CS into the water. This continued until the pH reached 7.53. It then decreased perhaps because of degradation of acidic amino acids in SF. Afterwards, the pH value again slightly increased. The mass percentage of CS was higher than that of SF, and hence the degradation of the alkaline CS resulted in the slight pH increase.


Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Change in the pH of the lysate and the degradation rate in the 40% SF-60% CS group.(A) pH fluctuated in a small range of 7.42~7.62; (B)The scaffold lost only 18.25% of its original weight after 64 days.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470591&req=5

pone.0128658.g007: Change in the pH of the lysate and the degradation rate in the 40% SF-60% CS group.(A) pH fluctuated in a small range of 7.42~7.62; (B)The scaffold lost only 18.25% of its original weight after 64 days.
Mentions: During the degradation process that lasted for two months, the pH fluctuated in a small range 7.42–7.62 (Fig 7A). In the first 12 days, the pH slowly increased, possibly due to the gradual degradation of the alkaline CS into the water. This continued until the pH reached 7.53. It then decreased perhaps because of degradation of acidic amino acids in SF. Afterwards, the pH value again slightly increased. The mass percentage of CS was higher than that of SF, and hence the degradation of the alkaline CS resulted in the slight pH increase.

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus