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Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

SEM images of SF/CS scaffold materials.(A) In the 20% SF-80% CS group, a porous network structure appeared. The thickness of the pore walls was uniform, yet the shape and size of the pores were not very regular, and the connectivity between the pores was poor. The mean pore diameter was 100±20.56 μm. (B) In the 40% SF -60% CS group, the pore size was uniform, and the pore walls were a single layer with a smooth surface and uniform thickness. The connectivity between the pores was good, and the pores themselves were round and relatively uniform with a mean pore size of 150±28.56 μm. (C) In the 60% SF-40% CS group, an irregular laminar curled structure appeared. The connectivity between the pores was relatively good. The mean pore size was 210±23.71 μm. (D) In the 80% SF-20% CS group, the pore size was large and the wall of each pore was not smooth, but composed of multiple irregular laminar structures. The curling was more notable than the 60% SF-40% CS group, and the pore shapes were not very uniform. The mean pore size was 300±23.43 μm.
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pone.0128658.g003: SEM images of SF/CS scaffold materials.(A) In the 20% SF-80% CS group, a porous network structure appeared. The thickness of the pore walls was uniform, yet the shape and size of the pores were not very regular, and the connectivity between the pores was poor. The mean pore diameter was 100±20.56 μm. (B) In the 40% SF -60% CS group, the pore size was uniform, and the pore walls were a single layer with a smooth surface and uniform thickness. The connectivity between the pores was good, and the pores themselves were round and relatively uniform with a mean pore size of 150±28.56 μm. (C) In the 60% SF-40% CS group, an irregular laminar curled structure appeared. The connectivity between the pores was relatively good. The mean pore size was 210±23.71 μm. (D) In the 80% SF-20% CS group, the pore size was large and the wall of each pore was not smooth, but composed of multiple irregular laminar structures. The curling was more notable than the 60% SF-40% CS group, and the pore shapes were not very uniform. The mean pore size was 300±23.43 μm.

Mentions: Examination of the internal structure of composite materials with different SF/CS mass ratios under EM (Fig 3) revealed a porous network structure with pore sizes ranging from 100 to 350 μm and clear connection between pores. The scaffold morphology of the 40% SF-60% CS group was the best among all groups. It had a uniform pore size, single-layer pore walls, smooth surface, uniform thickness, and good connectivity between the pores. The pores are round with diameters of 150±28.56 μm.


Characterization of Silk Fibroin/Chitosan 3D Porous Scaffold and In Vitro Cytology.

Zeng S, Liu L, Shi Y, Qiu J, Fang W, Rong M, Guo Z, Gao W - PLoS ONE (2015)

SEM images of SF/CS scaffold materials.(A) In the 20% SF-80% CS group, a porous network structure appeared. The thickness of the pore walls was uniform, yet the shape and size of the pores were not very regular, and the connectivity between the pores was poor. The mean pore diameter was 100±20.56 μm. (B) In the 40% SF -60% CS group, the pore size was uniform, and the pore walls were a single layer with a smooth surface and uniform thickness. The connectivity between the pores was good, and the pores themselves were round and relatively uniform with a mean pore size of 150±28.56 μm. (C) In the 60% SF-40% CS group, an irregular laminar curled structure appeared. The connectivity between the pores was relatively good. The mean pore size was 210±23.71 μm. (D) In the 80% SF-20% CS group, the pore size was large and the wall of each pore was not smooth, but composed of multiple irregular laminar structures. The curling was more notable than the 60% SF-40% CS group, and the pore shapes were not very uniform. The mean pore size was 300±23.43 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470591&req=5

pone.0128658.g003: SEM images of SF/CS scaffold materials.(A) In the 20% SF-80% CS group, a porous network structure appeared. The thickness of the pore walls was uniform, yet the shape and size of the pores were not very regular, and the connectivity between the pores was poor. The mean pore diameter was 100±20.56 μm. (B) In the 40% SF -60% CS group, the pore size was uniform, and the pore walls were a single layer with a smooth surface and uniform thickness. The connectivity between the pores was good, and the pores themselves were round and relatively uniform with a mean pore size of 150±28.56 μm. (C) In the 60% SF-40% CS group, an irregular laminar curled structure appeared. The connectivity between the pores was relatively good. The mean pore size was 210±23.71 μm. (D) In the 80% SF-20% CS group, the pore size was large and the wall of each pore was not smooth, but composed of multiple irregular laminar structures. The curling was more notable than the 60% SF-40% CS group, and the pore shapes were not very uniform. The mean pore size was 300±23.43 μm.
Mentions: Examination of the internal structure of composite materials with different SF/CS mass ratios under EM (Fig 3) revealed a porous network structure with pore sizes ranging from 100 to 350 μm and clear connection between pores. The scaffold morphology of the 40% SF-60% CS group was the best among all groups. It had a uniform pore size, single-layer pore walls, smooth surface, uniform thickness, and good connectivity between the pores. The pores are round with diameters of 150±28.56 μm.

Bottom Line: The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects.This favors the early adhesion, growth and proliferation of MG-63 cells.In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Guangdong Provincial Stomatological Hospital Affiliated to Southern Medical University, Guangzhou, 510280, Guangdong, P.R. China.

ABSTRACT
Bone tissue engineering is a powerful tool to treat bone defects caused by trauma, infection, tumors and other factors. Both silk fibroin (SF) and chitosan (CS) are non-toxic and have good biocompatibility, but are poor biological scaffolds when used alone. In this study, the microscopic structure and related properties of SF/CS composite scaffolds with different component ratios were examined. The scaffold material most suitable for osteoblast growth was determined, and these results offer an experimental basis for the future reconstruction of bone defects. First, via freeze-drying and chemical crosslinking methods, SF/CS composites with different component ratios were prepared and their structure was characterized. Changes in the internal structure of the SF and CS mixture were observed, confirming that the mutual modification between the two components was complete and stable. The internal structure of the composite material was porous and three-dimensional with a porosity above 90%. We next studied the pore size, swelling ratio, water absorption ratio, degradation and in vitro cell proliferation. For the 40% SF-60% CS group, the pore size of the scaffold was suitable for the growth of osteoblasts, and the rate of degradation was steady. This favors the early adhesion, growth and proliferation of MG-63 cells. In addition to good biocompatibility and satisfactory cell affinity, this material promotes the secretion of extracellular matrix materials by osteoblasts. Thus, 40% SF-60% CS is a good material for bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus