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Long-term, regular remote ischemic preconditioning improves endothelial function in patients with coronary heart disease.

Liang Y, Li YP, He F, Liu XQ, Zhang JY - Braz. J. Med. Biol. Res. (2015)

Bottom Line: The purpose of this study was to assess the beneficial effects of long-term regular RIPre on human arteries.RIPre activated STAT-3 and increased CD34(+) endothelial progenitor cell counts found in arteries.Long-term, regular RIPre improved endothelial function in patients with CHD, possibly due to STAT-3 activation, and this may have led to an increase in endothelial progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

ABSTRACT
Remote ischemic preconditioning (RIPre) can prevent myocardial injury. The purpose of this study was to assess the beneficial effects of long-term regular RIPre on human arteries. Forty patients scheduled for coronary artery bypass graft (CABG) surgery were assigned randomly to a RIPre group (n=20) or coronary heart disease (CHD) group (n=20). Twenty patients scheduled for mastectomy were enrolled as a control group. RIPre was achieved by occluding arterial blood flow 5 min with a mercury sphygmomanometer followed by a 5-min reperfusion period, and this was repeated 4 times. The RIPre procedure was repeated 3 times a day for 20 days. In all patients, arterial fragments discarded during surgery were collected to evaluate endothelial function by flow-mediated dilation (FMD), CD34(+) monocyte count, and endothelial nitric oxide synthase (eNOS expression). Phosphorylation levels of STAT-3 and Akt were also assayed to explore the underlying mechanisms. Compared with the CHD group, long-term regular RIPre significantly improved FMD after 20 days (8.5±2.4 vs 4.9±4.2%, P<0.05) and significantly reduced troponin after CABG surgery (0.72±0.31 and 1.64±0.19, P<0.05). RIPre activated STAT-3 and increased CD34(+) endothelial progenitor cell counts found in arteries. Long-term, regular RIPre improved endothelial function in patients with CHD, possibly due to STAT-3 activation, and this may have led to an increase in endothelial progenitor cells.

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A, Representative flow cytometry measurements ofCD34+ monocytes in blood before and after treatment in theA, control group; B, remote ischemicpreconditioning (RIPre) group; and C, coronary heart disease(CHD) group. D, CD34+ monocytes in blood before andafter treatment. Data are reported as means±SE. *P<0.05 vscontrol group; #P<0.05 vs RIPre group(Student-Newman-Keuls test).
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f05: A, Representative flow cytometry measurements ofCD34+ monocytes in blood before and after treatment in theA, control group; B, remote ischemicpreconditioning (RIPre) group; and C, coronary heart disease(CHD) group. D, CD34+ monocytes in blood before andafter treatment. Data are reported as means±SE. *P<0.05 vscontrol group; #P<0.05 vs RIPre group(Student-Newman-Keuls test).

Mentions: The arterial CD34+ cells of patients in the RIPre group exhibitedincreased immunofluorescence compared with those of patients in the CHD group. Thearterial CD34+ cells of patients in the control and CHD groups had thehighest and lowest immunofluorescence, respectively, and the immunofluorescenceobserved in the RIPre group was significantly higher than that in the CHD group(n=20, P<0.05; Figure 4). The number ofCD34+ cells was significantly higher in the control group than inpatients in the CHD or RIPre group. At the beginning of the study, the absolutenumber of CD34+ cells was significantly different (2.35±0.72 cells/µL,1.48±0.49 cells/µL and 1.56±0.37 cells/µL, P<0.05) in control group patients thanin patients in the CHD and RIPre groups. On the other hand, no significant differencewas noted in the number of CD34+ cells in the RIPre and CHD groups. On day20, the numbers of CD34+ cells in the RIPre and CHD groups weresignificantly different (1.79±0.31 cells/µL vs 1.34±0.20 cells/µL,P<0.05; Figure 5). Because the increase inendothelial progenitor cells (EPCs) and improvement in endothelial function may becorrelated, we carried out a regression analysis that revealed a slight butsignificantly positive correlation between FMD and the number of CD34+cells (r=0.46, P<0.05).


Long-term, regular remote ischemic preconditioning improves endothelial function in patients with coronary heart disease.

Liang Y, Li YP, He F, Liu XQ, Zhang JY - Braz. J. Med. Biol. Res. (2015)

A, Representative flow cytometry measurements ofCD34+ monocytes in blood before and after treatment in theA, control group; B, remote ischemicpreconditioning (RIPre) group; and C, coronary heart disease(CHD) group. D, CD34+ monocytes in blood before andafter treatment. Data are reported as means±SE. *P<0.05 vscontrol group; #P<0.05 vs RIPre group(Student-Newman-Keuls test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470317&req=5

f05: A, Representative flow cytometry measurements ofCD34+ monocytes in blood before and after treatment in theA, control group; B, remote ischemicpreconditioning (RIPre) group; and C, coronary heart disease(CHD) group. D, CD34+ monocytes in blood before andafter treatment. Data are reported as means±SE. *P<0.05 vscontrol group; #P<0.05 vs RIPre group(Student-Newman-Keuls test).
Mentions: The arterial CD34+ cells of patients in the RIPre group exhibitedincreased immunofluorescence compared with those of patients in the CHD group. Thearterial CD34+ cells of patients in the control and CHD groups had thehighest and lowest immunofluorescence, respectively, and the immunofluorescenceobserved in the RIPre group was significantly higher than that in the CHD group(n=20, P<0.05; Figure 4). The number ofCD34+ cells was significantly higher in the control group than inpatients in the CHD or RIPre group. At the beginning of the study, the absolutenumber of CD34+ cells was significantly different (2.35±0.72 cells/µL,1.48±0.49 cells/µL and 1.56±0.37 cells/µL, P<0.05) in control group patients thanin patients in the CHD and RIPre groups. On the other hand, no significant differencewas noted in the number of CD34+ cells in the RIPre and CHD groups. On day20, the numbers of CD34+ cells in the RIPre and CHD groups weresignificantly different (1.79±0.31 cells/µL vs 1.34±0.20 cells/µL,P<0.05; Figure 5). Because the increase inendothelial progenitor cells (EPCs) and improvement in endothelial function may becorrelated, we carried out a regression analysis that revealed a slight butsignificantly positive correlation between FMD and the number of CD34+cells (r=0.46, P<0.05).

Bottom Line: The purpose of this study was to assess the beneficial effects of long-term regular RIPre on human arteries.RIPre activated STAT-3 and increased CD34(+) endothelial progenitor cell counts found in arteries.Long-term, regular RIPre improved endothelial function in patients with CHD, possibly due to STAT-3 activation, and this may have led to an increase in endothelial progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

ABSTRACT
Remote ischemic preconditioning (RIPre) can prevent myocardial injury. The purpose of this study was to assess the beneficial effects of long-term regular RIPre on human arteries. Forty patients scheduled for coronary artery bypass graft (CABG) surgery were assigned randomly to a RIPre group (n=20) or coronary heart disease (CHD) group (n=20). Twenty patients scheduled for mastectomy were enrolled as a control group. RIPre was achieved by occluding arterial blood flow 5 min with a mercury sphygmomanometer followed by a 5-min reperfusion period, and this was repeated 4 times. The RIPre procedure was repeated 3 times a day for 20 days. In all patients, arterial fragments discarded during surgery were collected to evaluate endothelial function by flow-mediated dilation (FMD), CD34(+) monocyte count, and endothelial nitric oxide synthase (eNOS expression). Phosphorylation levels of STAT-3 and Akt were also assayed to explore the underlying mechanisms. Compared with the CHD group, long-term regular RIPre significantly improved FMD after 20 days (8.5±2.4 vs 4.9±4.2%, P<0.05) and significantly reduced troponin after CABG surgery (0.72±0.31 and 1.64±0.19, P<0.05). RIPre activated STAT-3 and increased CD34(+) endothelial progenitor cell counts found in arteries. Long-term, regular RIPre improved endothelial function in patients with CHD, possibly due to STAT-3 activation, and this may have led to an increase in endothelial progenitor cells.

Show MeSH
Related in: MedlinePlus