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Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning.

Cui SQ, Wang Q, Zheng Y, Xiao B, Sun HW, Gu XL, Zhang YC, Fu CH, Dong PX, Wang XM - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA.In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels.In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

View Article: PubMed Central - PubMed

Affiliation: China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.

ABSTRACT
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

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Detection of glutamic acid (Glu) and gamma amino butyric acid (GABA) byhigh-performance liquid chromatography (HPLC).A,B, Glu content of the cortex andhippocampus. C,D, GABA content of the cortexand hippocampus. E,F, Glu/GABA ratio in thecortex and hippocampus. *P<0.05, **P<0.01 vs controlgroup; #P<0.05, ##P<0.01 vs modelgroup (one-way ANOVA).
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f05: Detection of glutamic acid (Glu) and gamma amino butyric acid (GABA) byhigh-performance liquid chromatography (HPLC).A,B, Glu content of the cortex andhippocampus. C,D, GABA content of the cortexand hippocampus. E,F, Glu/GABA ratio in thecortex and hippocampus. *P<0.05, **P<0.01 vs controlgroup; #P<0.05, ##P<0.01 vs modelgroup (one-way ANOVA).

Mentions: Compared with the control group, the Glu and GABA levels of the cortex andhippocampus were significantly reduced in the model group (Figure 5, P<0.05 and P<0.01, respectively). Puerarintreatment significantly reversed the reduction of GABA in both the cortex (P<0.05)and hippocampus (P<0.01), but reversed only the reduction of Glu in thehippocampus (P<0.05). The Glu/GABA ratio in the cortex was significantly higher inthe model group than in both the control group (P<0.05) and the puerarin group(P<0.01). Puerarin treatment significantly inhibited the increase of the Glu/GABAratio in the hippocampus compared with the model group (P<0.01).


Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning.

Cui SQ, Wang Q, Zheng Y, Xiao B, Sun HW, Gu XL, Zhang YC, Fu CH, Dong PX, Wang XM - Braz. J. Med. Biol. Res. (2015)

Detection of glutamic acid (Glu) and gamma amino butyric acid (GABA) byhigh-performance liquid chromatography (HPLC).A,B, Glu content of the cortex andhippocampus. C,D, GABA content of the cortexand hippocampus. E,F, Glu/GABA ratio in thecortex and hippocampus. *P<0.05, **P<0.01 vs controlgroup; #P<0.05, ##P<0.01 vs modelgroup (one-way ANOVA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470310&req=5

f05: Detection of glutamic acid (Glu) and gamma amino butyric acid (GABA) byhigh-performance liquid chromatography (HPLC).A,B, Glu content of the cortex andhippocampus. C,D, GABA content of the cortexand hippocampus. E,F, Glu/GABA ratio in thecortex and hippocampus. *P<0.05, **P<0.01 vs controlgroup; #P<0.05, ##P<0.01 vs modelgroup (one-way ANOVA).
Mentions: Compared with the control group, the Glu and GABA levels of the cortex andhippocampus were significantly reduced in the model group (Figure 5, P<0.05 and P<0.01, respectively). Puerarintreatment significantly reversed the reduction of GABA in both the cortex (P<0.05)and hippocampus (P<0.01), but reversed only the reduction of Glu in thehippocampus (P<0.05). The Glu/GABA ratio in the cortex was significantly higher inthe model group than in both the control group (P<0.05) and the puerarin group(P<0.01). Puerarin treatment significantly inhibited the increase of the Glu/GABAratio in the hippocampus compared with the model group (P<0.01).

Bottom Line: Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA.In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels.In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

View Article: PubMed Central - PubMed

Affiliation: China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.

ABSTRACT
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

Show MeSH
Related in: MedlinePlus