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Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning.

Cui SQ, Wang Q, Zheng Y, Xiao B, Sun HW, Gu XL, Zhang YC, Fu CH, Dong PX, Wang XM - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA.In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels.In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

View Article: PubMed Central - PubMed

Affiliation: China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.

ABSTRACT
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

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Results of the Morris water maze test. A, Escape latency:time to reach the hidden platform. B, Escape distance: lengthof the swimming path taken to find the platform. C, Crosstimes: times the mice passed through the platform. D, Totalswimming distance in controlled time (1 min). E, Typicalescape route (the swimming path of mice) in the 3 groups. *P<0.05,**P<0.01 vs control group; #P<0.05,##P<0.01 vs model group (one-wayANOVA).
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f02: Results of the Morris water maze test. A, Escape latency:time to reach the hidden platform. B, Escape distance: lengthof the swimming path taken to find the platform. C, Crosstimes: times the mice passed through the platform. D, Totalswimming distance in controlled time (1 min). E, Typicalescape route (the swimming path of mice) in the 3 groups. *P<0.05,**P<0.01 vs control group; #P<0.05,##P<0.01 vs model group (one-wayANOVA).

Mentions: Puerarin improved the spatial learning memory disorder of mice with chronic alcoholpoisoning. In the spatial navigation test, the escape latency and escape distance ofthe model group were significantly longer than in the control group (P<0.05 andP<0.01, respectively; Figure 2A and B). Whentreated with puerarin, the escape latency on days 3, 4, and 5 and the escape distanceon days 3 and 5 days in the puerarin group were significantly reduced compared withthe control group (P<0.05 and P<0.01, respectively). In the spatial probetrial, the cross times and total swimming distance of the model group weresignificantly shorter than those in the control group, but in the puerarin group, thecross times and total swimming distance were similar to those in the control groupand significantly longer than those in the model group (Figure 2C and D, P<0.05). The escape routes might reflect theescape strategy of mice. As shown in Figure 2E,the escape route of the mice in the control group was short and direct, while in themodel group, the route was complex and wandering. In the puerarin group, the routewas better than that in the model group, but still worse than in the controlgroup.


Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning.

Cui SQ, Wang Q, Zheng Y, Xiao B, Sun HW, Gu XL, Zhang YC, Fu CH, Dong PX, Wang XM - Braz. J. Med. Biol. Res. (2015)

Results of the Morris water maze test. A, Escape latency:time to reach the hidden platform. B, Escape distance: lengthof the swimming path taken to find the platform. C, Crosstimes: times the mice passed through the platform. D, Totalswimming distance in controlled time (1 min). E, Typicalescape route (the swimming path of mice) in the 3 groups. *P<0.05,**P<0.01 vs control group; #P<0.05,##P<0.01 vs model group (one-wayANOVA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470310&req=5

f02: Results of the Morris water maze test. A, Escape latency:time to reach the hidden platform. B, Escape distance: lengthof the swimming path taken to find the platform. C, Crosstimes: times the mice passed through the platform. D, Totalswimming distance in controlled time (1 min). E, Typicalescape route (the swimming path of mice) in the 3 groups. *P<0.05,**P<0.01 vs control group; #P<0.05,##P<0.01 vs model group (one-wayANOVA).
Mentions: Puerarin improved the spatial learning memory disorder of mice with chronic alcoholpoisoning. In the spatial navigation test, the escape latency and escape distance ofthe model group were significantly longer than in the control group (P<0.05 andP<0.01, respectively; Figure 2A and B). Whentreated with puerarin, the escape latency on days 3, 4, and 5 and the escape distanceon days 3 and 5 days in the puerarin group were significantly reduced compared withthe control group (P<0.05 and P<0.01, respectively). In the spatial probetrial, the cross times and total swimming distance of the model group weresignificantly shorter than those in the control group, but in the puerarin group, thecross times and total swimming distance were similar to those in the control groupand significantly longer than those in the model group (Figure 2C and D, P<0.05). The escape routes might reflect theescape strategy of mice. As shown in Figure 2E,the escape route of the mice in the control group was short and direct, while in themodel group, the route was complex and wandering. In the puerarin group, the routewas better than that in the model group, but still worse than in the controlgroup.

Bottom Line: Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA.In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels.In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

View Article: PubMed Central - PubMed

Affiliation: China Shandong Provincial Engineering Laboratory of New Pharmaceutical Excipients, Sustained and Controlled Release Technology, College of Medicine and Nursing, Dezhou University, Dezhou, China.

ABSTRACT
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.

Show MeSH
Related in: MedlinePlus