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Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis.

Godoy CR, Levy D, Giampaoli V, Chamone DA, Bydlowski SP, Pereira J - Braz. J. Med. Biol. Res. (2015)

Bottom Line: There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05).In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145).In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.

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Flow cytometry analyses of endothelial cells in venous blood samplesfrom chronic myeloid leukemia (CML) patients in blast crisis.A1, A2 and A3,corresponding isotypic control. B1, endothelial precursorcells (EPCs) expressingCD45-/dim/CD146+/CD133+; andB2,CD45-/dim/CD146+/CD34+.B3, Characterization of activated mature circulatingendothelial cells expressingCD45-/dim/CD146+/CD62e+ and mCEC(CD45-/dim/CD146+/CD62e-).
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f02: Flow cytometry analyses of endothelial cells in venous blood samplesfrom chronic myeloid leukemia (CML) patients in blast crisis.A1, A2 and A3,corresponding isotypic control. B1, endothelial precursorcells (EPCs) expressingCD45-/dim/CD146+/CD133+; andB2,CD45-/dim/CD146+/CD34+.B3, Characterization of activated mature circulatingendothelial cells expressingCD45-/dim/CD146+/CD62e+ and mCEC(CD45-/dim/CD146+/CD62e-).

Mentions: We first selected all living cells in a side-scatter (SSC)/CD45 plot to excludeplatelets, dead cells, and debris. Then, we gated around a region corresponding toCD45- negative and low SSC in order to exclude hematopoietic cells(Figure 1) (14-16). Subsequently,we looked for CD146, CD34, CD62e, and CD133 expression in other 2D fluorescenceplots from R2. EPCs were considered asCD45-/dim/CD146+/CD133+/CD34+/CD62e-/+and mature (m)CECs asCD45-/dim/CD146+/CD62e+/-/CD133-/CD34-(Figure 2). The presence of the CD62eantigen characterized activated EPCs and mCECs. CECs were reported as a percentageof the total events after the exclusion of debris. To prevent bias, onlypercentage values were used in statistical analyses because the leukocyte countwas highly variable in each group. The medians were 6.2×109/L in thecontrol group, 21.2×109/L in the CP-CML group, 87.5×109/L inthe BC-CML group, and 6.1×109/L in the AP-CML group.


Circulating endothelial cells are increased in chronic myeloid leukemia blast crisis.

Godoy CR, Levy D, Giampaoli V, Chamone DA, Bydlowski SP, Pereira J - Braz. J. Med. Biol. Res. (2015)

Flow cytometry analyses of endothelial cells in venous blood samplesfrom chronic myeloid leukemia (CML) patients in blast crisis.A1, A2 and A3,corresponding isotypic control. B1, endothelial precursorcells (EPCs) expressingCD45-/dim/CD146+/CD133+; andB2,CD45-/dim/CD146+/CD34+.B3, Characterization of activated mature circulatingendothelial cells expressingCD45-/dim/CD146+/CD62e+ and mCEC(CD45-/dim/CD146+/CD62e-).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470309&req=5

f02: Flow cytometry analyses of endothelial cells in venous blood samplesfrom chronic myeloid leukemia (CML) patients in blast crisis.A1, A2 and A3,corresponding isotypic control. B1, endothelial precursorcells (EPCs) expressingCD45-/dim/CD146+/CD133+; andB2,CD45-/dim/CD146+/CD34+.B3, Characterization of activated mature circulatingendothelial cells expressingCD45-/dim/CD146+/CD62e+ and mCEC(CD45-/dim/CD146+/CD62e-).
Mentions: We first selected all living cells in a side-scatter (SSC)/CD45 plot to excludeplatelets, dead cells, and debris. Then, we gated around a region corresponding toCD45- negative and low SSC in order to exclude hematopoietic cells(Figure 1) (14-16). Subsequently,we looked for CD146, CD34, CD62e, and CD133 expression in other 2D fluorescenceplots from R2. EPCs were considered asCD45-/dim/CD146+/CD133+/CD34+/CD62e-/+and mature (m)CECs asCD45-/dim/CD146+/CD62e+/-/CD133-/CD34-(Figure 2). The presence of the CD62eantigen characterized activated EPCs and mCECs. CECs were reported as a percentageof the total events after the exclusion of debris. To prevent bias, onlypercentage values were used in statistical analyses because the leukocyte countwas highly variable in each group. The medians were 6.2×109/L in thecontrol group, 21.2×109/L in the CP-CML group, 87.5×109/L inthe BC-CML group, and 6.1×109/L in the AP-CML group.

Bottom Line: There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05).In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145).In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.

View Article: PubMed Central - PubMed

Affiliation: Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil.

ABSTRACT
We measured circulating endothelial precursor cells (EPCs), activated circulating endothelial cells (aCECs), and mature circulating endothelial cells (mCECs) using four-color multiparametric flow cytometry in the peripheral blood of 84 chronic myeloid leukemia (CML) patients and 65 healthy controls; and vascular endothelial growth factor (VEGF) by quantitative real-time PCR in 50 CML patients and 32 healthy controls. Because of an increase in mCECs, the median percentage of CECs in CML blast crisis (0.0146%) was significantly higher than in healthy subjects (0.0059%, P<0.01) and in the accelerated phase (0.0059%, P=0.01). There were no significant differences in the percentages of CECs in chronic- or active-phase patients and healthy subjects (P>0.05). In addition, VEGF gene expression was significantly higher in all phases of CML: 0.245 in blast crisis, 0.320 in the active phase, and 0.330 in chronic phase patients than it was in healthy subjects (0.145). In conclusion, CML in blast crisis had increased levels of CECs and VEGF gene expression, which may serve as markers of disease progression and may become targets for the management of CML.

Show MeSH
Related in: MedlinePlus