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Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase.

Li Y, Chen LJ, Jiang F, Yang Y, Wang XX, Zhang Z, Li Z, Li L - Braz. J. Med. Biol. Res. (2015)

Bottom Line: However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved.We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs).In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

ABSTRACT
Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H2O2-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage.

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Caffeic acid (CaA)-induced improvement of cell viability and protectionagainst DNA damage: involvement of reactive oxygen species (ROS) and extracellularsignal-regulated kinase (ERK) signaling.
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f07: Caffeic acid (CaA)-induced improvement of cell viability and protectionagainst DNA damage: involvement of reactive oxygen species (ROS) and extracellularsignal-regulated kinase (ERK) signaling.

Mentions: A low concentration of CaA increased the viability of human liver L-02 cells, attenuatedthe H2O2-associated reduction of cell viability, and decreased theoccurrence of H2O2-induced DSBs. Compared with cells exposed toH2O2, CaA-treated cells generated lower levels of ROS thatinduced ERK signaling pathway. Inhibition of ERK signaling blocked the CaA-inducedimprovement of cell viability and protection against DNA damage caused byH2O2 treatment (Figure7).


Caffeic acid improves cell viability and protects against DNA damage: involvement of reactive oxygen species and extracellular signal-regulated kinase.

Li Y, Chen LJ, Jiang F, Yang Y, Wang XX, Zhang Z, Li Z, Li L - Braz. J. Med. Biol. Res. (2015)

Caffeic acid (CaA)-induced improvement of cell viability and protectionagainst DNA damage: involvement of reactive oxygen species (ROS) and extracellularsignal-regulated kinase (ERK) signaling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470308&req=5

f07: Caffeic acid (CaA)-induced improvement of cell viability and protectionagainst DNA damage: involvement of reactive oxygen species (ROS) and extracellularsignal-regulated kinase (ERK) signaling.
Mentions: A low concentration of CaA increased the viability of human liver L-02 cells, attenuatedthe H2O2-associated reduction of cell viability, and decreased theoccurrence of H2O2-induced DSBs. Compared with cells exposed toH2O2, CaA-treated cells generated lower levels of ROS thatinduced ERK signaling pathway. Inhibition of ERK signaling blocked the CaA-inducedimprovement of cell viability and protection against DNA damage caused byH2O2 treatment (Figure7).

Bottom Line: However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved.We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs).In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Nanjing Medical University, Nanjing, China.

ABSTRACT
Hormesis is an adaptive response to a variety of oxidative stresses that renders cells resistant to harmful doses of stressing agents. Caffeic acid (CaA) is an important antioxidant that has protective effects against DNA damage caused by reactive oxygen species (ROS). However, whether CaA-induced protection is a hormetic effect remains unknown, as is the molecular mechanism that is involved. We found that a low concentration (10 μM) of CaA increased human liver L-02 cell viability, attenuated hydrogen peroxide (H2O2)-mediated decreases in cell viability, and decreased the extent of H2O2-induced DNA double-strand breaks (DSBs). In L-02 cells exposed to H2O2, CaA treatment reduced ROS levels, which might have played a protective role. CaA also activated the extracellular signal-regulated kinase (ERK) signal pathway in a time-dependent manner. Inhibition of ERK by its inhibitor U0126 or by its specific small interfering RNA (siRNA) blocked the CaA-induced improvement in cell viability and the protective effects against H2O2-mediated DNA damage. This study adds to the understanding of the antioxidant effects of CaA by identifying a novel molecular mechanism of enhanced cell viability and protection against DNA damage.

Show MeSH
Related in: MedlinePlus