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Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model.

Li NQ, Yang J, Cui L, Ma N, Zhang L, Hao LR - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR).Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis.This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

ABSTRACT
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.

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Western blot analysis of abnormal cytokine expression in mice afterunilateral ureteral obstruction (UUO). TGFβ: transforming growth factor beta;HGF: hepatocyte growth-promoting factor; BMP7: bone morphogenetic protein-7;CTGF: connective tissue growth factor; GAPDH: glyceraldehyde 3-phosphatedehydrogenase.
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f03: Western blot analysis of abnormal cytokine expression in mice afterunilateral ureteral obstruction (UUO). TGFβ: transforming growth factor beta;HGF: hepatocyte growth-promoting factor; BMP7: bone morphogenetic protein-7;CTGF: connective tissue growth factor; GAPDH: glyceraldehyde 3-phosphatedehydrogenase.

Mentions: Protein expression of Tgfβ and Ctgf, both promotersof renal fibrosis, significantly increased 3 days and 1 and 2 weeks after surgerycompared to the control group (P<0.01), while protein expression ofBmp7, an inhibitor of renal fibrosis, significantly decreased at3 days and 1 and 2 weeks after surgery compared to the control group (P<0.01).Expression of Hgf, an inhibitor of renal fibrosis, significantlyincreased 1 and 2 weeks after surgery compared to the control group (P<0.01).Expression of Igf2, the host gene for miR-483, significantlyincreased 3 days and 1 and 2 weeks after surgery compared to the control group(P<0.01; Figure 3).


Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model.

Li NQ, Yang J, Cui L, Ma N, Zhang L, Hao LR - Braz. J. Med. Biol. Res. (2015)

Western blot analysis of abnormal cytokine expression in mice afterunilateral ureteral obstruction (UUO). TGFβ: transforming growth factor beta;HGF: hepatocyte growth-promoting factor; BMP7: bone morphogenetic protein-7;CTGF: connective tissue growth factor; GAPDH: glyceraldehyde 3-phosphatedehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470306&req=5

f03: Western blot analysis of abnormal cytokine expression in mice afterunilateral ureteral obstruction (UUO). TGFβ: transforming growth factor beta;HGF: hepatocyte growth-promoting factor; BMP7: bone morphogenetic protein-7;CTGF: connective tissue growth factor; GAPDH: glyceraldehyde 3-phosphatedehydrogenase.
Mentions: Protein expression of Tgfβ and Ctgf, both promotersof renal fibrosis, significantly increased 3 days and 1 and 2 weeks after surgerycompared to the control group (P<0.01), while protein expression ofBmp7, an inhibitor of renal fibrosis, significantly decreased at3 days and 1 and 2 weeks after surgery compared to the control group (P<0.01).Expression of Hgf, an inhibitor of renal fibrosis, significantlyincreased 1 and 2 weeks after surgery compared to the control group (P<0.01).Expression of Igf2, the host gene for miR-483, significantlyincreased 3 days and 1 and 2 weeks after surgery compared to the control group(P<0.01; Figure 3).

Bottom Line: Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR).Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis.This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

ABSTRACT
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.

Show MeSH
Related in: MedlinePlus