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Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model.

Li NQ, Yang J, Cui L, Ma N, Zhang L, Hao LR - Braz. J. Med. Biol. Res. (2015)

Bottom Line: Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR).Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis.This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

ABSTRACT
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.

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Related in: MedlinePlus

Expression of and relationships among miR-483, miR-483*, and the mRNAs forBmp7, Igf2, Tgfβ,Hgf and Ctgf. Real-time PCR was performedon the samples to evaluate the expression of miR-483, miR-483*, and severalcytokine mRNAs, including Bmp7, Igf2,Tgfβ, Hgf and Ctgf. d:days, w: week. *P<0.05, compared to the control (Dunnett's multiplecomparison test).
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f02: Expression of and relationships among miR-483, miR-483*, and the mRNAs forBmp7, Igf2, Tgfβ,Hgf and Ctgf. Real-time PCR was performedon the samples to evaluate the expression of miR-483, miR-483*, and severalcytokine mRNAs, including Bmp7, Igf2,Tgfβ, Hgf and Ctgf. d:days, w: week. *P<0.05, compared to the control (Dunnett's multiplecomparison test).

Mentions: Real-time PCR was performed on the samples to evaluate the expression of miR-483,miR-483* and several cytokine mRNAs, including Bmp7,Igf2, Tgfβ, Hgf, andCtgf. In addition, expression relationships among the abovemiRNAs and mRNAs were investigated in kidney tissue with varying extents ofinterstitial fibrosis. The expression of miR-483 and miR-483* was significantlyhigher at 1 and 2 weeks after surgery compared to the control group (P<0.05), butwas not significantly lower 3 days after surgery (P>0.05; Figure 2A). Igf2 and Hgf mRNAexpression increased significantly 1 and 2 weeks after surgery compared to thecontrol group (P<0.05), but was not significantly increased 3 days after surgery(P>0.05). Tgfβ and Ctgf mRNA expressionincreased significantly 3 days and 1 and 2 weeks after surgery compared to thecontrol group (P<0.05), while Bmp7 mRNA expression decreased(P<0.05; Figure 2B). Using Pearsoncorrelation analysis, miR-483 expression positively correlated withIgf2, Tgfβ, Hgf, andCtgf expression (r=0.555, 0.573, 0.841, 0.76;Igf2 P<0.05, others P<0.01) and negatively correlated withBmp7 expression (r=-0.72, P<0.01; Figure 2C). In addition, miR-483* expression positivelycorrelated with Igf2, Tgfβ, Hgf,and Ctgf expression (r=0.805, 0.623, 0.792, 0.874; P<0.01) andnegatively correlated with Bmp7 expression (r=-0.741, P<0.01;Figure 2D).


Expression of intronic miRNAs and their host gene Igf2 in a murine unilateral ureteral obstruction model.

Li NQ, Yang J, Cui L, Ma N, Zhang L, Hao LR - Braz. J. Med. Biol. Res. (2015)

Expression of and relationships among miR-483, miR-483*, and the mRNAs forBmp7, Igf2, Tgfβ,Hgf and Ctgf. Real-time PCR was performedon the samples to evaluate the expression of miR-483, miR-483*, and severalcytokine mRNAs, including Bmp7, Igf2,Tgfβ, Hgf and Ctgf. d:days, w: week. *P<0.05, compared to the control (Dunnett's multiplecomparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470306&req=5

f02: Expression of and relationships among miR-483, miR-483*, and the mRNAs forBmp7, Igf2, Tgfβ,Hgf and Ctgf. Real-time PCR was performedon the samples to evaluate the expression of miR-483, miR-483*, and severalcytokine mRNAs, including Bmp7, Igf2,Tgfβ, Hgf and Ctgf. d:days, w: week. *P<0.05, compared to the control (Dunnett's multiplecomparison test).
Mentions: Real-time PCR was performed on the samples to evaluate the expression of miR-483,miR-483* and several cytokine mRNAs, including Bmp7,Igf2, Tgfβ, Hgf, andCtgf. In addition, expression relationships among the abovemiRNAs and mRNAs were investigated in kidney tissue with varying extents ofinterstitial fibrosis. The expression of miR-483 and miR-483* was significantlyhigher at 1 and 2 weeks after surgery compared to the control group (P<0.05), butwas not significantly lower 3 days after surgery (P>0.05; Figure 2A). Igf2 and Hgf mRNAexpression increased significantly 1 and 2 weeks after surgery compared to thecontrol group (P<0.05), but was not significantly increased 3 days after surgery(P>0.05). Tgfβ and Ctgf mRNA expressionincreased significantly 3 days and 1 and 2 weeks after surgery compared to thecontrol group (P<0.05), while Bmp7 mRNA expression decreased(P<0.05; Figure 2B). Using Pearsoncorrelation analysis, miR-483 expression positively correlated withIgf2, Tgfβ, Hgf, andCtgf expression (r=0.555, 0.573, 0.841, 0.76;Igf2 P<0.05, others P<0.01) and negatively correlated withBmp7 expression (r=-0.72, P<0.01; Figure 2C). In addition, miR-483* expression positivelycorrelated with Igf2, Tgfβ, Hgf,and Ctgf expression (r=0.805, 0.623, 0.792, 0.874; P<0.01) andnegatively correlated with Bmp7 expression (r=-0.741, P<0.01;Figure 2D).

Bottom Line: Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR).Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis.This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model.

View Article: PubMed Central - PubMed

Affiliation: Nephrology Department, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

ABSTRACT
The objective of this study was to determine the expression of miR-483 and miR-483* and the relationship among them, their host gene (Igf2), and other cytokines in a murine model of renal fibrosis. The extent of renal fibrosis was visualized using Masson staining, and fibrosis was scored 3 days and 1 and 2 weeks after unilateral ureteral obstruction (UUO). Expression of miR-483, miR-483* and various cytokine mRNAs was detected by real-time polymerase chain reaction (PCR). Expression of miR-483 and miR-483* was significantly upregulated in the UUO model, particularly miR-483 expression was the greatest 2 weeks after surgery. Additionally, miR-483 and miR-483* expression negatively correlated with Bmp7 expression and positively correlated with Igf2, Tgfβ, Hgf, and Ctgf expression, as determined by Pearson's correlation analysis. Hgf expression significantly increased at 1 and 2 weeks after the surgery compared to the control group. This study showed that miR-483 and miR-483* expression was upregulated in a murine UUO model. These data suggest that miR-483 and miR-483* play a role in renal fibrosis and that miR-483* may interact with miR-483 in renal fibrosis. Thus, these miRNAs may play a role in the pathogenesis of renal fibrosis and coexpression of their host gene Igf2.

Show MeSH
Related in: MedlinePlus