Limits...
Expression Analyses Revealed Thymic Stromal Co-Transporter/Slc46A2 Is in Stem Cell Populations and Is a Putative Tumor Suppressor.

Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG - Mol. Cells (2015)

Bottom Line: By searching variations in the expression levels, TSCOT is positively associated with Grhl3 and Irf6.Surprisingly, we found TSCOT expression in the lung is diminished in lung cancers, suggesting TSCOT may be involved in the suppression of lung tumor development.Based on these results, a model for TEC differentiation from the stem cells was proposed in context of multiple epithelial organ formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Inha University, Incheon 402-720, Korea.

ABSTRACT
By combining conventional single cell analysis with flow cytometry and public database searches with bioinformatics tools, we extended the expression profiling of thymic stromal cotransporter (TSCOT), Slc46A2/Ly110, that was shown to be expressed in bipotent precursor and cortical thymic epithelial cells. Genome scale analysis verified TSCOT expression in thymic tissue- and cell type- specific fashion and is also expressed in some other epithelial tissues including skin and lung. Coexpression profiling with genes, Foxn1 and Hoxa3, revealed the role of TSCOT during the organogenesis. TSCOT expression was detected in all thymic epithelial cells (TECs), but not in the CD31(+) endothelial cell lineage in fetal thymus. In addition, ABC transporter-dependent side population and Sca-1(+) fetal TEC populations both contain TSCOT-expressing cells, indicating TEC stem cells express TSCOT. TSCOT expression was identified as early as in differentiating embryonic stem cells. TSCOT expression is not under the control of Foxn1 since TSCOT is present in the thymic rudiment of nude mice. By searching variations in the expression levels, TSCOT is positively associated with Grhl3 and Irf6. Cytokines such as IL1b, IL22 and IL24 are the potential regulators of the TSCOT expression. Surprisingly, we found TSCOT expression in the lung is diminished in lung cancers, suggesting TSCOT may be involved in the suppression of lung tumor development. Based on these results, a model for TEC differentiation from the stem cells was proposed in context of multiple epithelial organ formation.

No MeSH data available.


Related in: MedlinePlus

Tissue specific expression of TSCOT reveals Foxn1 independency. (A) RT-PCR analysis of adult nude tissue. (B) Gene expression profiles from embryonic day 17.5 IRF6 KO and wild type mouse skin (GSE5800). Right panel: Comparison of TSCOT expression from skin between IRF6 KO mice and wild-type (P value < 0.0001****). (C) Gene expression profiles from embryonic day 18 GRHL3 KO and wild type mouse skin (GSE7381). Right panel: Comparison of TSCOT expression from skin between GRHL3 KO mice and wild-type (P value: 0.0042**). High, Red; Middle, Black; Low, Green. (D) TSCOT expression changes in human epidermal keratinocytes after treatment of KGF and various cytokines (GSE7216). Significant changes are compared to untreated cells (P < 0.0001****, P: 0.0025**, P: 0.0217*). Y axis are arbiturary units of process data.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4469913&req=5

f5-molce-38-6-548: Tissue specific expression of TSCOT reveals Foxn1 independency. (A) RT-PCR analysis of adult nude tissue. (B) Gene expression profiles from embryonic day 17.5 IRF6 KO and wild type mouse skin (GSE5800). Right panel: Comparison of TSCOT expression from skin between IRF6 KO mice and wild-type (P value < 0.0001****). (C) Gene expression profiles from embryonic day 18 GRHL3 KO and wild type mouse skin (GSE7381). Right panel: Comparison of TSCOT expression from skin between GRHL3 KO mice and wild-type (P value: 0.0042**). High, Red; Middle, Black; Low, Green. (D) TSCOT expression changes in human epidermal keratinocytes after treatment of KGF and various cytokines (GSE7216). Significant changes are compared to untreated cells (P < 0.0001****, P: 0.0025**, P: 0.0217*). Y axis are arbiturary units of process data.

Mentions: Because FOXN1 was considered as a putative TEC key transcription factor, we searched for TSCOT expression in the remaining thymic rudiment of nude mouse. In RNA prepared from several tissues, TSCOT expression was found in the thymic rudiment of nude mice (Fig. 5A). RNA samples that were not treated with reverse transcriptase did not generate any bands (data not shown). This result is consistent with the conclusion derived from the gene profiling analyses.


Expression Analyses Revealed Thymic Stromal Co-Transporter/Slc46A2 Is in Stem Cell Populations and Is a Putative Tumor Suppressor.

Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG - Mol. Cells (2015)

Tissue specific expression of TSCOT reveals Foxn1 independency. (A) RT-PCR analysis of adult nude tissue. (B) Gene expression profiles from embryonic day 17.5 IRF6 KO and wild type mouse skin (GSE5800). Right panel: Comparison of TSCOT expression from skin between IRF6 KO mice and wild-type (P value < 0.0001****). (C) Gene expression profiles from embryonic day 18 GRHL3 KO and wild type mouse skin (GSE7381). Right panel: Comparison of TSCOT expression from skin between GRHL3 KO mice and wild-type (P value: 0.0042**). High, Red; Middle, Black; Low, Green. (D) TSCOT expression changes in human epidermal keratinocytes after treatment of KGF and various cytokines (GSE7216). Significant changes are compared to untreated cells (P < 0.0001****, P: 0.0025**, P: 0.0217*). Y axis are arbiturary units of process data.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4469913&req=5

f5-molce-38-6-548: Tissue specific expression of TSCOT reveals Foxn1 independency. (A) RT-PCR analysis of adult nude tissue. (B) Gene expression profiles from embryonic day 17.5 IRF6 KO and wild type mouse skin (GSE5800). Right panel: Comparison of TSCOT expression from skin between IRF6 KO mice and wild-type (P value < 0.0001****). (C) Gene expression profiles from embryonic day 18 GRHL3 KO and wild type mouse skin (GSE7381). Right panel: Comparison of TSCOT expression from skin between GRHL3 KO mice and wild-type (P value: 0.0042**). High, Red; Middle, Black; Low, Green. (D) TSCOT expression changes in human epidermal keratinocytes after treatment of KGF and various cytokines (GSE7216). Significant changes are compared to untreated cells (P < 0.0001****, P: 0.0025**, P: 0.0217*). Y axis are arbiturary units of process data.
Mentions: Because FOXN1 was considered as a putative TEC key transcription factor, we searched for TSCOT expression in the remaining thymic rudiment of nude mouse. In RNA prepared from several tissues, TSCOT expression was found in the thymic rudiment of nude mice (Fig. 5A). RNA samples that were not treated with reverse transcriptase did not generate any bands (data not shown). This result is consistent with the conclusion derived from the gene profiling analyses.

Bottom Line: By searching variations in the expression levels, TSCOT is positively associated with Grhl3 and Irf6.Surprisingly, we found TSCOT expression in the lung is diminished in lung cancers, suggesting TSCOT may be involved in the suppression of lung tumor development.Based on these results, a model for TEC differentiation from the stem cells was proposed in context of multiple epithelial organ formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Inha University, Incheon 402-720, Korea.

ABSTRACT
By combining conventional single cell analysis with flow cytometry and public database searches with bioinformatics tools, we extended the expression profiling of thymic stromal cotransporter (TSCOT), Slc46A2/Ly110, that was shown to be expressed in bipotent precursor and cortical thymic epithelial cells. Genome scale analysis verified TSCOT expression in thymic tissue- and cell type- specific fashion and is also expressed in some other epithelial tissues including skin and lung. Coexpression profiling with genes, Foxn1 and Hoxa3, revealed the role of TSCOT during the organogenesis. TSCOT expression was detected in all thymic epithelial cells (TECs), but not in the CD31(+) endothelial cell lineage in fetal thymus. In addition, ABC transporter-dependent side population and Sca-1(+) fetal TEC populations both contain TSCOT-expressing cells, indicating TEC stem cells express TSCOT. TSCOT expression was identified as early as in differentiating embryonic stem cells. TSCOT expression is not under the control of Foxn1 since TSCOT is present in the thymic rudiment of nude mice. By searching variations in the expression levels, TSCOT is positively associated with Grhl3 and Irf6. Cytokines such as IL1b, IL22 and IL24 are the potential regulators of the TSCOT expression. Surprisingly, we found TSCOT expression in the lung is diminished in lung cancers, suggesting TSCOT may be involved in the suppression of lung tumor development. Based on these results, a model for TEC differentiation from the stem cells was proposed in context of multiple epithelial organ formation.

No MeSH data available.


Related in: MedlinePlus