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Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

Hänggi P, Telezhkin V, Kemp PJ, Schmugge M, Gassmann M, Goede JS, Speer O, Bogdanova A - Am. J. Physiol., Cell Physiol. (2015)

Bottom Line: Kinetics of this switch in NMDAR properties and abundance varied markedly from donor to donor.Despite this variability, NMDARs were essential for survival of EPCs in any subject tested.Our findings indicate that NMDARs have a dual role during erythropoiesis, supporting survival of polychromatic erythroblasts and contributing to the Ca(2+) homeostasis from the orthochromatic erythroblast stage to circulating red blood cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary Physiology, University of Zurich, Zurich, Switzerland; Division of Hematology University Hospital Zurich, Zurich, Switzerland; University Children's Hospital, Zurich, Switzerland;

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Donor dependence of Ca2+ influx, receptor properties, and GRIN2A expression during hematopoiesis. mRNA expression was normalized to GAPDH. A: correlation between GRIN2A mRNA expression and relative change in geometric mean fluorescence (GMF) in the stimulated EPCs. Correlation coefficient was calculated by the change in GMF triggered by the agonists and the relative mRNA expression from six donors. B: correlation of current density and relative GRIN2A mRNA expression (n = 4). C: correlation of deactivation time and relative GRIN2A mRNA expression from four donors. Data are means ± SD; linear (A and B) and exponential (C) functions were used for curve fitting.
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Figure 5: Donor dependence of Ca2+ influx, receptor properties, and GRIN2A expression during hematopoiesis. mRNA expression was normalized to GAPDH. A: correlation between GRIN2A mRNA expression and relative change in geometric mean fluorescence (GMF) in the stimulated EPCs. Correlation coefficient was calculated by the change in GMF triggered by the agonists and the relative mRNA expression from six donors. B: correlation of current density and relative GRIN2A mRNA expression (n = 4). C: correlation of deactivation time and relative GRIN2A mRNA expression from four donors. Data are means ± SD; linear (A and B) and exponential (C) functions were used for curve fitting.

Mentions: We have monitored interindividual variability in the relative mRNA expression of GRIN2A at day 8 and the relative change of intracellular Ca2+ concentration after NMDAR activation that was recorded as a shift in geometric mean fluorescence (GMF). GRIN2A expression of individual donors correlated with the amplitude of changes in the intracellular Ca2+ levels after NMDAR stimulation (Fig. 5A).


Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

Hänggi P, Telezhkin V, Kemp PJ, Schmugge M, Gassmann M, Goede JS, Speer O, Bogdanova A - Am. J. Physiol., Cell Physiol. (2015)

Donor dependence of Ca2+ influx, receptor properties, and GRIN2A expression during hematopoiesis. mRNA expression was normalized to GAPDH. A: correlation between GRIN2A mRNA expression and relative change in geometric mean fluorescence (GMF) in the stimulated EPCs. Correlation coefficient was calculated by the change in GMF triggered by the agonists and the relative mRNA expression from six donors. B: correlation of current density and relative GRIN2A mRNA expression (n = 4). C: correlation of deactivation time and relative GRIN2A mRNA expression from four donors. Data are means ± SD; linear (A and B) and exponential (C) functions were used for curve fitting.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4469746&req=5

Figure 5: Donor dependence of Ca2+ influx, receptor properties, and GRIN2A expression during hematopoiesis. mRNA expression was normalized to GAPDH. A: correlation between GRIN2A mRNA expression and relative change in geometric mean fluorescence (GMF) in the stimulated EPCs. Correlation coefficient was calculated by the change in GMF triggered by the agonists and the relative mRNA expression from six donors. B: correlation of current density and relative GRIN2A mRNA expression (n = 4). C: correlation of deactivation time and relative GRIN2A mRNA expression from four donors. Data are means ± SD; linear (A and B) and exponential (C) functions were used for curve fitting.
Mentions: We have monitored interindividual variability in the relative mRNA expression of GRIN2A at day 8 and the relative change of intracellular Ca2+ concentration after NMDAR activation that was recorded as a shift in geometric mean fluorescence (GMF). GRIN2A expression of individual donors correlated with the amplitude of changes in the intracellular Ca2+ levels after NMDAR stimulation (Fig. 5A).

Bottom Line: Kinetics of this switch in NMDAR properties and abundance varied markedly from donor to donor.Despite this variability, NMDARs were essential for survival of EPCs in any subject tested.Our findings indicate that NMDARs have a dual role during erythropoiesis, supporting survival of polychromatic erythroblasts and contributing to the Ca(2+) homeostasis from the orthochromatic erythroblast stage to circulating red blood cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary Physiology, University of Zurich, Zurich, Switzerland; Division of Hematology University Hospital Zurich, Zurich, Switzerland; University Children's Hospital, Zurich, Switzerland;

Show MeSH
Related in: MedlinePlus