Limits...
Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation.

Wang L, Zhu Y, Hu W, Zhang X, Cai C, Guo W - PLoS ONE (2015)

Bottom Line: Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1.Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant.Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
Analysis of mutants and gene expression patterns provides a powerful approach for investigating genes involved in key stages of plant fiber development. In this study, lintless-fuzzless XinWX and linted-fuzzless XinFLM with a single genetic locus difference for lint were used to identify differentially expressed genes. Scanning electron microscopy showed fiber initiation in XinFLM at 0 days post anthesis (DPA). Fiber transcriptional profiling of the lines at three initiation developmental stages (-1, 0, 1 DPA) was performed using an oligonucleotide microarray. Loop comparisons of the differentially expressed genes within and between the lines was carried out, and functional classification and enrichment analysis showed that gene expression patterns during fiber initiation were heavily associated with hormone metabolism, transcription factor regulation, lipid transport, and asparagine biosynthetic processes, as previously reported. Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1. Real time-quantitative PCR (RT-qPCR) analysis in different fiber mutant lines revealed that AOCs were up-regulated higher at -1 DPA in lintless-fuzzless than that in linted-fuzzless and linted-fuzzed materials, and transcription of the AOCs was increased under jasmonic acid (JA) treatment. Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant. Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation. Parallel up-regulation of AOCs expression may be important for normal fiber initiation development, while overproduction of AOCs might disrupt normal fiber development.

No MeSH data available.


Related in: MedlinePlus

Differentially expressed genes involved in JA biosynthesis and signal transduction.Mixtures of ovules and fibers at -1, 0, and 1 DPA are shown in x-axis. Error bars indicate ± SD. Significant differences at the 0.05 and 0.01 probability levels are represented by * and **, respectively. The expression level was derived from triplicate experiments. Three biological replicates were used for calculation.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4469610&req=5

pone.0129854.g008: Differentially expressed genes involved in JA biosynthesis and signal transduction.Mixtures of ovules and fibers at -1, 0, and 1 DPA are shown in x-axis. Error bars indicate ± SD. Significant differences at the 0.05 and 0.01 probability levels are represented by * and **, respectively. The expression level was derived from triplicate experiments. Three biological replicates were used for calculation.

Mentions: To determine whether JA biosynthesis was co-regulated during fiber initiation, seven genes (AOS, 28K_069_H08 and 28K_156_D09; LOX, 28K_275_B02 and 28K_150_B07; OPR, 28K_299_E12; GH3, 28K_086_B03 and 28K_236_A10), which were differentially expressed between XinWX and XinFLM in the microarrays and their homologs reported previously were involved in JA biosynthesis in other plants [41], were subjected to RT-qPCR analysis (Fig 8). Other than 28K_086_B03 which showed significant expression differences at 1 DPA, the other six DEGs showed significantly higher expression in XinWX than in XinFLM between -1 and 1 DPA, especially at -1 DPA. Of these, two ESTs (28K_086_B03 and 28K_236_A10), encoding GH3 proteins, are known to be important for jasmonate signaling [35, 36]. GH3 genes are induced by auxin and conjugate JA with amino acids. GH3.11 conjugates JA with isoleucine, and the resultant jasmonoyl-L-isoleucine (JA-Ile) is a crucial signalling molecule for the major jasmonate signaling pathway involving COI1 [42]. GH3.3, GH3.5, and GH3.6 also conjugate JA to various amino acids and by doing so regulate JA homeostasis in Arabidopsis adventitious root initiation [43]. These results and the expression profiles of the AOCs suggested that the JA biosynthesis pathway is up-regulated during fiber initiation in the fuzzless-lintless mutant, who may result in overproduction of JA in XinWX compared to XinFLM.


Comparative Transcriptomics Reveals Jasmonic Acid-Associated Metabolism Related to Cotton Fiber Initiation.

Wang L, Zhu Y, Hu W, Zhang X, Cai C, Guo W - PLoS ONE (2015)

Differentially expressed genes involved in JA biosynthesis and signal transduction.Mixtures of ovules and fibers at -1, 0, and 1 DPA are shown in x-axis. Error bars indicate ± SD. Significant differences at the 0.05 and 0.01 probability levels are represented by * and **, respectively. The expression level was derived from triplicate experiments. Three biological replicates were used for calculation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4469610&req=5

pone.0129854.g008: Differentially expressed genes involved in JA biosynthesis and signal transduction.Mixtures of ovules and fibers at -1, 0, and 1 DPA are shown in x-axis. Error bars indicate ± SD. Significant differences at the 0.05 and 0.01 probability levels are represented by * and **, respectively. The expression level was derived from triplicate experiments. Three biological replicates were used for calculation.
Mentions: To determine whether JA biosynthesis was co-regulated during fiber initiation, seven genes (AOS, 28K_069_H08 and 28K_156_D09; LOX, 28K_275_B02 and 28K_150_B07; OPR, 28K_299_E12; GH3, 28K_086_B03 and 28K_236_A10), which were differentially expressed between XinWX and XinFLM in the microarrays and their homologs reported previously were involved in JA biosynthesis in other plants [41], were subjected to RT-qPCR analysis (Fig 8). Other than 28K_086_B03 which showed significant expression differences at 1 DPA, the other six DEGs showed significantly higher expression in XinWX than in XinFLM between -1 and 1 DPA, especially at -1 DPA. Of these, two ESTs (28K_086_B03 and 28K_236_A10), encoding GH3 proteins, are known to be important for jasmonate signaling [35, 36]. GH3 genes are induced by auxin and conjugate JA with amino acids. GH3.11 conjugates JA with isoleucine, and the resultant jasmonoyl-L-isoleucine (JA-Ile) is a crucial signalling molecule for the major jasmonate signaling pathway involving COI1 [42]. GH3.3, GH3.5, and GH3.6 also conjugate JA to various amino acids and by doing so regulate JA homeostasis in Arabidopsis adventitious root initiation [43]. These results and the expression profiles of the AOCs suggested that the JA biosynthesis pathway is up-regulated during fiber initiation in the fuzzless-lintless mutant, who may result in overproduction of JA in XinWX compared to XinFLM.

Bottom Line: Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1.Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant.Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing, Jiangsu, China.

ABSTRACT
Analysis of mutants and gene expression patterns provides a powerful approach for investigating genes involved in key stages of plant fiber development. In this study, lintless-fuzzless XinWX and linted-fuzzless XinFLM with a single genetic locus difference for lint were used to identify differentially expressed genes. Scanning electron microscopy showed fiber initiation in XinFLM at 0 days post anthesis (DPA). Fiber transcriptional profiling of the lines at three initiation developmental stages (-1, 0, 1 DPA) was performed using an oligonucleotide microarray. Loop comparisons of the differentially expressed genes within and between the lines was carried out, and functional classification and enrichment analysis showed that gene expression patterns during fiber initiation were heavily associated with hormone metabolism, transcription factor regulation, lipid transport, and asparagine biosynthetic processes, as previously reported. Further, four members of the allene-oxide cyclase (AOC) family that function in jasmonate biosynthesis were parallel up-regulation in fiber initiation, especially at -1 DPA, compared to other tissues and organs in linted-fuzzed TM-1. Real time-quantitative PCR (RT-qPCR) analysis in different fiber mutant lines revealed that AOCs were up-regulated higher at -1 DPA in lintless-fuzzless than that in linted-fuzzless and linted-fuzzed materials, and transcription of the AOCs was increased under jasmonic acid (JA) treatment. Expression analysis of JA biosynthesis-associated genes between XinWX and XinFLM showed that they were up-regulated during fiber initiation in the fuzzless-lintless mutant. Taken together, jasmonic acid-associated metabolism was related to cotton fiber initiation. Parallel up-regulation of AOCs expression may be important for normal fiber initiation development, while overproduction of AOCs might disrupt normal fiber development.

No MeSH data available.


Related in: MedlinePlus