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CdSe/ZnS Quantum Dots-Labeled Mesenchymal Stem Cells for Targeted Fluorescence Imaging of Pancreas Tissues and Therapy of Type 1 Diabetic Rats.

Liu H, Tang W, Li C, Lv P, Wang Z, Liu Y, Zhang C, Bao Y, Chen H, Meng X, Song Y, Xia X, Pan F, Cui D, Shi Y - Nanoscale Res Lett (2015)

Bottom Line: However, the in vivo distribution and therapeutic effects of transplanted MSCs are not clarified well.Statistical differences existed between the blood glucose levels of the diabetic rat control group and MSC-injected diabetic rat group (p < 0.01), and the MSC-injected diabetic rat group displayed lower blood glucose levels.In conclusion, CdSe/ZnS-labeled MSCs can target in vivo pancreas tissues in diabetic rats, and significantly reduce the blood glucose levels in diabetic rats, and own potential application in therapy of diabetic patients in the near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology and Metabolism, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, People's Republic of China.

ABSTRACT
Mesenchymal stem cells (MSCs) have been used for therapy of type 1 diabetes mellitus. However, the in vivo distribution and therapeutic effects of transplanted MSCs are not clarified well. Herein, we reported that CdSe/ZnS quantum dots-labeled MSCs were prepared for targeted fluorescence imaging and therapy of pancreas tissues in rat models with type 1 diabetes. CdSe/ZnS quantum dots were synthesized, their biocompatibility was evaluated, and then, the appropriate concentration of quantum dots was selected to label MSCs. CdSe/ZnS quantum dots-labeled MSCs were injected into mouse models with type 1 diabetes via tail vessel and then were observed by using the Bruker In-Vivo F PRO system, and the blood glucose levels were monitored for 8 weeks. Results showed that prepared CdSe/ZnS quantum dots owned good biocompatibility. Significant differences existed in distribution of quantum dots-labeled MSCs between normal control rats and diabetic rats (p < 0.05). The ratios of the fluorescence intensity (RFI) analysis showed an accumulation rate of MSCs in the pancreas of rats in the diabetes group which was about 32 %, while that in the normal control group rats was about 18 %. The blood glucose levels were also monitored for 8 weeks after quantum dots-labeled MSC injection. Statistical differences existed between the blood glucose levels of the diabetic rat control group and MSC-injected diabetic rat group (p < 0.01), and the MSC-injected diabetic rat group displayed lower blood glucose levels. In conclusion, CdSe/ZnS-labeled MSCs can target in vivo pancreas tissues in diabetic rats, and significantly reduce the blood glucose levels in diabetic rats, and own potential application in therapy of diabetic patients in the near future.

No MeSH data available.


Related in: MedlinePlus

Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers. a During the onset of growth (P0-7th day), MSCs showed diverse morphologies including ovoid, bipolar, and large, flattened morphology. b In later passages (P4-4th day), most of these MSCs exhibited large, flattened, or fibroblast-like morphology
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Fig1: Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers. a During the onset of growth (P0-7th day), MSCs showed diverse morphologies including ovoid, bipolar, and large, flattened morphology. b In later passages (P4-4th day), most of these MSCs exhibited large, flattened, or fibroblast-like morphology

Mentions: Rat MSCs were isolated and cultured. MSCs attached to the culture flasks sparsely and displayed a fibroblast-like, spindle-shaped morphology during the initial days of incubation. After 3 ~ 4 days of incubation, proliferation started and the cells gradually grew into small colonies. One week later, colonies with different sizes increased in number. As growth continued, adjacent colonies interconnected with each other and a monolayer confluence was obtained after 12 ~ 15 days of incubation. In later passages, MSCs exhibited large, flattened, or fibroblast-like morphology (Fig. 1a, b).Fig. 1


CdSe/ZnS Quantum Dots-Labeled Mesenchymal Stem Cells for Targeted Fluorescence Imaging of Pancreas Tissues and Therapy of Type 1 Diabetic Rats.

Liu H, Tang W, Li C, Lv P, Wang Z, Liu Y, Zhang C, Bao Y, Chen H, Meng X, Song Y, Xia X, Pan F, Cui D, Shi Y - Nanoscale Res Lett (2015)

Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers. a During the onset of growth (P0-7th day), MSCs showed diverse morphologies including ovoid, bipolar, and large, flattened morphology. b In later passages (P4-4th day), most of these MSCs exhibited large, flattened, or fibroblast-like morphology
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4469594&req=5

Fig1: Phase-contrast photo micrographs of cultures showing MSC lines at various passage numbers. a During the onset of growth (P0-7th day), MSCs showed diverse morphologies including ovoid, bipolar, and large, flattened morphology. b In later passages (P4-4th day), most of these MSCs exhibited large, flattened, or fibroblast-like morphology
Mentions: Rat MSCs were isolated and cultured. MSCs attached to the culture flasks sparsely and displayed a fibroblast-like, spindle-shaped morphology during the initial days of incubation. After 3 ~ 4 days of incubation, proliferation started and the cells gradually grew into small colonies. One week later, colonies with different sizes increased in number. As growth continued, adjacent colonies interconnected with each other and a monolayer confluence was obtained after 12 ~ 15 days of incubation. In later passages, MSCs exhibited large, flattened, or fibroblast-like morphology (Fig. 1a, b).Fig. 1

Bottom Line: However, the in vivo distribution and therapeutic effects of transplanted MSCs are not clarified well.Statistical differences existed between the blood glucose levels of the diabetic rat control group and MSC-injected diabetic rat group (p < 0.01), and the MSC-injected diabetic rat group displayed lower blood glucose levels.In conclusion, CdSe/ZnS-labeled MSCs can target in vivo pancreas tissues in diabetic rats, and significantly reduce the blood glucose levels in diabetic rats, and own potential application in therapy of diabetic patients in the near future.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology and Metabolism, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, People's Republic of China.

ABSTRACT
Mesenchymal stem cells (MSCs) have been used for therapy of type 1 diabetes mellitus. However, the in vivo distribution and therapeutic effects of transplanted MSCs are not clarified well. Herein, we reported that CdSe/ZnS quantum dots-labeled MSCs were prepared for targeted fluorescence imaging and therapy of pancreas tissues in rat models with type 1 diabetes. CdSe/ZnS quantum dots were synthesized, their biocompatibility was evaluated, and then, the appropriate concentration of quantum dots was selected to label MSCs. CdSe/ZnS quantum dots-labeled MSCs were injected into mouse models with type 1 diabetes via tail vessel and then were observed by using the Bruker In-Vivo F PRO system, and the blood glucose levels were monitored for 8 weeks. Results showed that prepared CdSe/ZnS quantum dots owned good biocompatibility. Significant differences existed in distribution of quantum dots-labeled MSCs between normal control rats and diabetic rats (p < 0.05). The ratios of the fluorescence intensity (RFI) analysis showed an accumulation rate of MSCs in the pancreas of rats in the diabetes group which was about 32 %, while that in the normal control group rats was about 18 %. The blood glucose levels were also monitored for 8 weeks after quantum dots-labeled MSC injection. Statistical differences existed between the blood glucose levels of the diabetic rat control group and MSC-injected diabetic rat group (p < 0.01), and the MSC-injected diabetic rat group displayed lower blood glucose levels. In conclusion, CdSe/ZnS-labeled MSCs can target in vivo pancreas tissues in diabetic rats, and significantly reduce the blood glucose levels in diabetic rats, and own potential application in therapy of diabetic patients in the near future.

No MeSH data available.


Related in: MedlinePlus