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Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferation.

Huang Z, Li Y, Niu L, Xiao Y, Pu X, Zheng H, Qian M - Biomed Eng Online (2015)

Bottom Line: The expressions of MCP-1 and CCR2 in the arterial tissues were detected using reverse transcription polymerase chain reaction (RT-PCR) and analyzed by semi-quantitative method.The MCP-1 reached to the peak on the first day, but decreased gradually later on.Expressions of CCR2 mRNA began to increase on the first day and reached to the peak on the 7th day, but then started to decrease gradually until 28th day when we can still detect it.

View Article: PubMed Central - PubMed

Affiliation: Emergency Department, Peking University Shenzhen Hospital, Shenzhen, 518036, China. dochzg@163.com.

ABSTRACT

Objective: The dynamic expressions of monocyte chemo attractant protein-1 (MCP-1) and CC chamomile receptor 2 (CCR2) after balloon injury and their effects in intimal proliferation were discussed. In this study, the expression of MCP-1 and its receptor during the intimal proliferation in rat artery after balloon injury were studied.

Methods: Using the model of balloon injury of rats' arteries, the changes of intimal proliferation were observed with optical microscopy and the expressions of MCP-1 and CCR2 at different times were examined with the methods of RT-PCR and immunohistochemistry. The expressions of MCP-1 and CCR2 in the arterial tissues were detected using reverse transcription polymerase chain reaction (RT-PCR) and analyzed by semi-quantitative method.

Results: The expressions of MCP-1 and CCR2 mRNA began to gradually increase after balloon injury. The MCP-1 reached to the peak on the first day, but decreased gradually later on. Expressions of CCR2 mRNA began to increase on the first day and reached to the peak on the 7th day, but then started to decrease gradually until 28th day when we can still detect it. The expressions of MCP-1 proteins began to increase gradually after balloon injury and were obviously detected in the VSMC on the 4th and 7th day, until 14th day when we can still detect it clearly in the proliferating intima.

Conclusion: The dynamic expressions of MCP-1, MCP-1 proteins and CCR2 mRNA after balloon injury were shown to play an important role in intimal proliferation.

No MeSH data available.


Related in: MedlinePlus

Detection results of immunohistochemistry of MCP-1 proteins in the vessel walls. A: negative expressions of MCP-1 proteins in sham group (Immunohistochemical SP method, DAB color, × 400); (B): positive expressions of MCP-1 proteins in cytoplasm of vascular smooth muscle cells near the internal elastic plates after balloon injury for 4 days (Immunohistochemical SP method, DAB color, × 400); (C): positive expressions of MCP-1 proteins in cytoplasm of smooth muscle cells in media after balloon injury for 7 days (Immunohistochemical SP method, DAB color, × 400); (D): positive expressions of MCP-1 proteins in neointimal after balloon injury for 14 days (Immunohistochemical SP method, DAB color, × 400); (E): positive expressions of MCP-1 proteins in neointimal after balloon injury for 28 days (Immunohistochemical SP method, DAB color, × 400).
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Fig4: Detection results of immunohistochemistry of MCP-1 proteins in the vessel walls. A: negative expressions of MCP-1 proteins in sham group (Immunohistochemical SP method, DAB color, × 400); (B): positive expressions of MCP-1 proteins in cytoplasm of vascular smooth muscle cells near the internal elastic plates after balloon injury for 4 days (Immunohistochemical SP method, DAB color, × 400); (C): positive expressions of MCP-1 proteins in cytoplasm of smooth muscle cells in media after balloon injury for 7 days (Immunohistochemical SP method, DAB color, × 400); (D): positive expressions of MCP-1 proteins in neointimal after balloon injury for 14 days (Immunohistochemical SP method, DAB color, × 400); (E): positive expressions of MCP-1 proteins in neointimal after balloon injury for 28 days (Immunohistochemical SP method, DAB color, × 400).

Mentions: The results of immunohistochemistry of MCP-1 proteins in the vessel walls were shown in Figure 4. There was no expression of MCP-1 proteins in sham group (Figure 4A). After balloon injury, expressions of MCP-1 proteins gradually increased and the expressions in cytoplasm of vascular smooth muscle cells near the internal elastic plates increased on the 4th day (Figure 4B). On the 7th day, large amounts of expressions of MCP-1 proteins could be detected in cytoplasm of smooth muscle cells in media (Figure 4D). On the 14th day, expressions in neointimal reached to the peak. on the 28th day, large amounts of expressions could be detected in neointimal.Figure 4


Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferation.

Huang Z, Li Y, Niu L, Xiao Y, Pu X, Zheng H, Qian M - Biomed Eng Online (2015)

Detection results of immunohistochemistry of MCP-1 proteins in the vessel walls. A: negative expressions of MCP-1 proteins in sham group (Immunohistochemical SP method, DAB color, × 400); (B): positive expressions of MCP-1 proteins in cytoplasm of vascular smooth muscle cells near the internal elastic plates after balloon injury for 4 days (Immunohistochemical SP method, DAB color, × 400); (C): positive expressions of MCP-1 proteins in cytoplasm of smooth muscle cells in media after balloon injury for 7 days (Immunohistochemical SP method, DAB color, × 400); (D): positive expressions of MCP-1 proteins in neointimal after balloon injury for 14 days (Immunohistochemical SP method, DAB color, × 400); (E): positive expressions of MCP-1 proteins in neointimal after balloon injury for 28 days (Immunohistochemical SP method, DAB color, × 400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4469411&req=5

Fig4: Detection results of immunohistochemistry of MCP-1 proteins in the vessel walls. A: negative expressions of MCP-1 proteins in sham group (Immunohistochemical SP method, DAB color, × 400); (B): positive expressions of MCP-1 proteins in cytoplasm of vascular smooth muscle cells near the internal elastic plates after balloon injury for 4 days (Immunohistochemical SP method, DAB color, × 400); (C): positive expressions of MCP-1 proteins in cytoplasm of smooth muscle cells in media after balloon injury for 7 days (Immunohistochemical SP method, DAB color, × 400); (D): positive expressions of MCP-1 proteins in neointimal after balloon injury for 14 days (Immunohistochemical SP method, DAB color, × 400); (E): positive expressions of MCP-1 proteins in neointimal after balloon injury for 28 days (Immunohistochemical SP method, DAB color, × 400).
Mentions: The results of immunohistochemistry of MCP-1 proteins in the vessel walls were shown in Figure 4. There was no expression of MCP-1 proteins in sham group (Figure 4A). After balloon injury, expressions of MCP-1 proteins gradually increased and the expressions in cytoplasm of vascular smooth muscle cells near the internal elastic plates increased on the 4th day (Figure 4B). On the 7th day, large amounts of expressions of MCP-1 proteins could be detected in cytoplasm of smooth muscle cells in media (Figure 4D). On the 14th day, expressions in neointimal reached to the peak. on the 28th day, large amounts of expressions could be detected in neointimal.Figure 4

Bottom Line: The expressions of MCP-1 and CCR2 in the arterial tissues were detected using reverse transcription polymerase chain reaction (RT-PCR) and analyzed by semi-quantitative method.The MCP-1 reached to the peak on the first day, but decreased gradually later on.Expressions of CCR2 mRNA began to increase on the first day and reached to the peak on the 7th day, but then started to decrease gradually until 28th day when we can still detect it.

View Article: PubMed Central - PubMed

Affiliation: Emergency Department, Peking University Shenzhen Hospital, Shenzhen, 518036, China. dochzg@163.com.

ABSTRACT

Objective: The dynamic expressions of monocyte chemo attractant protein-1 (MCP-1) and CC chamomile receptor 2 (CCR2) after balloon injury and their effects in intimal proliferation were discussed. In this study, the expression of MCP-1 and its receptor during the intimal proliferation in rat artery after balloon injury were studied.

Methods: Using the model of balloon injury of rats' arteries, the changes of intimal proliferation were observed with optical microscopy and the expressions of MCP-1 and CCR2 at different times were examined with the methods of RT-PCR and immunohistochemistry. The expressions of MCP-1 and CCR2 in the arterial tissues were detected using reverse transcription polymerase chain reaction (RT-PCR) and analyzed by semi-quantitative method.

Results: The expressions of MCP-1 and CCR2 mRNA began to gradually increase after balloon injury. The MCP-1 reached to the peak on the first day, but decreased gradually later on. Expressions of CCR2 mRNA began to increase on the first day and reached to the peak on the 7th day, but then started to decrease gradually until 28th day when we can still detect it. The expressions of MCP-1 proteins began to increase gradually after balloon injury and were obviously detected in the VSMC on the 4th and 7th day, until 14th day when we can still detect it clearly in the proliferating intima.

Conclusion: The dynamic expressions of MCP-1, MCP-1 proteins and CCR2 mRNA after balloon injury were shown to play an important role in intimal proliferation.

No MeSH data available.


Related in: MedlinePlus