Limits...
Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size.

Gronowicz G, Secor ER, Flynn JR, Jellison ER, Kuhn LT - Evid Based Complement Alternat Med (2015)

Bottom Line: No significant differences were found in body weight gain or tumor size.Metastasis was significantly reduced in the TT-treated mice compared to mock-treated mice.TT significantly reduced IL-1-a, MIG, IL-1b, and MIP-2 to control/vehicle levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA.

ABSTRACT
Evidence-based integrative medicine therapies have been introduced to promote wellness and offset side-effects from cancer treatment. Energy medicine is an integrative medicine technique using the human biofield to promote well-being. The biofield therapy chosen for study was Therapeutic Touch (TT). Breast cancer tumors were initiated in mice by injection of metastatic 66cl4 mammary carcinoma cells. The control group received only vehicle. TT or mock treatments were performed twice a week for 10 minutes. Two experienced TT practitioners alternated treatments. At 26 days, metastasis to popliteal lymph nodes was determined by clonogenic assay. Changes in immune function were measured by analysis of serum cytokines and by fluorescent activated cells sorting (FACS) of immune cells from the spleen and lymph nodes. No significant differences were found in body weight gain or tumor size. Metastasis was significantly reduced in the TT-treated mice compared to mock-treated mice. Cancer significantly elevated eleven cytokines. TT significantly reduced IL-1-a, MIG, IL-1b, and MIP-2 to control/vehicle levels. FACS demonstrated that TT significantly reduced specific splenic lymphocyte subsets and macrophages were significantly elevated with cancer. Human biofield therapy had no significant effect on primary tumor but produced significant effects on metastasis and immune responses in a mouse breast cancer model.

No MeSH data available.


Related in: MedlinePlus

Proliferation and apoptosis in the primary tumor. In (a) the normal morphology of the foot is seen in a PBS-treated mouse (PBS). Panels (b) and (c) demonstrate at successively higher magnifications the change in the foot injected with 66cl4 cells from a mouse breast carcinoma (CA group). Mostly undifferentiated tumor tissue is found. In panel (d) PCNA-stained cells are seen in the TT-treated tumor (arrows), and the graph (e) shows no significant differences in proliferation between CA and TT groups. Apoptosis was assessed by immunohistochemistry (arrows) of the TT-treated tumor (f) and quantified in the graph (g) showing no significant differences between mice from the CA and TT groups. Bar = 250 μm in (a) and (b). Bar = 50 μm in (c), (d), and (f).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4465772&req=5

fig1: Proliferation and apoptosis in the primary tumor. In (a) the normal morphology of the foot is seen in a PBS-treated mouse (PBS). Panels (b) and (c) demonstrate at successively higher magnifications the change in the foot injected with 66cl4 cells from a mouse breast carcinoma (CA group). Mostly undifferentiated tumor tissue is found. In panel (d) PCNA-stained cells are seen in the TT-treated tumor (arrows), and the graph (e) shows no significant differences in proliferation between CA and TT groups. Apoptosis was assessed by immunohistochemistry (arrows) of the TT-treated tumor (f) and quantified in the graph (g) showing no significant differences between mice from the CA and TT groups. Bar = 250 μm in (a) and (b). Bar = 50 μm in (c), (d), and (f).

Mentions: Figure 1(a) shows the histology of the normal mouse foot from a representative PBS-injected mouse. Foot pad tumor histology (Figures 1(b) and 1(c)) contain mostly undifferentiated tissue. PCNA immunocytochemistry demonstrated numerous proliferating cells (Figure 1(d)) in the tumor tissue, but there were no significant differences in PCNA-stained cell numbers between the TT- and the mock-treated tumor (CA) (Figure 1(e)). There were fewer apoptotic cells (Figure 1(f)) than proliferating cells; however, in a similar manner, no significant differences in apoptotic cell numbers were found between the TT and CA (Figure 1(g)).


Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size.

Gronowicz G, Secor ER, Flynn JR, Jellison ER, Kuhn LT - Evid Based Complement Alternat Med (2015)

Proliferation and apoptosis in the primary tumor. In (a) the normal morphology of the foot is seen in a PBS-treated mouse (PBS). Panels (b) and (c) demonstrate at successively higher magnifications the change in the foot injected with 66cl4 cells from a mouse breast carcinoma (CA group). Mostly undifferentiated tumor tissue is found. In panel (d) PCNA-stained cells are seen in the TT-treated tumor (arrows), and the graph (e) shows no significant differences in proliferation between CA and TT groups. Apoptosis was assessed by immunohistochemistry (arrows) of the TT-treated tumor (f) and quantified in the graph (g) showing no significant differences between mice from the CA and TT groups. Bar = 250 μm in (a) and (b). Bar = 50 μm in (c), (d), and (f).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465772&req=5

fig1: Proliferation and apoptosis in the primary tumor. In (a) the normal morphology of the foot is seen in a PBS-treated mouse (PBS). Panels (b) and (c) demonstrate at successively higher magnifications the change in the foot injected with 66cl4 cells from a mouse breast carcinoma (CA group). Mostly undifferentiated tumor tissue is found. In panel (d) PCNA-stained cells are seen in the TT-treated tumor (arrows), and the graph (e) shows no significant differences in proliferation between CA and TT groups. Apoptosis was assessed by immunohistochemistry (arrows) of the TT-treated tumor (f) and quantified in the graph (g) showing no significant differences between mice from the CA and TT groups. Bar = 250 μm in (a) and (b). Bar = 50 μm in (c), (d), and (f).
Mentions: Figure 1(a) shows the histology of the normal mouse foot from a representative PBS-injected mouse. Foot pad tumor histology (Figures 1(b) and 1(c)) contain mostly undifferentiated tissue. PCNA immunocytochemistry demonstrated numerous proliferating cells (Figure 1(d)) in the tumor tissue, but there were no significant differences in PCNA-stained cell numbers between the TT- and the mock-treated tumor (CA) (Figure 1(e)). There were fewer apoptotic cells (Figure 1(f)) than proliferating cells; however, in a similar manner, no significant differences in apoptotic cell numbers were found between the TT and CA (Figure 1(g)).

Bottom Line: No significant differences were found in body weight gain or tumor size.Metastasis was significantly reduced in the TT-treated mice compared to mock-treated mice.TT significantly reduced IL-1-a, MIG, IL-1b, and MIP-2 to control/vehicle levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, University of Connecticut Health Center, Farmington, CT 06030, USA.

ABSTRACT
Evidence-based integrative medicine therapies have been introduced to promote wellness and offset side-effects from cancer treatment. Energy medicine is an integrative medicine technique using the human biofield to promote well-being. The biofield therapy chosen for study was Therapeutic Touch (TT). Breast cancer tumors were initiated in mice by injection of metastatic 66cl4 mammary carcinoma cells. The control group received only vehicle. TT or mock treatments were performed twice a week for 10 minutes. Two experienced TT practitioners alternated treatments. At 26 days, metastasis to popliteal lymph nodes was determined by clonogenic assay. Changes in immune function were measured by analysis of serum cytokines and by fluorescent activated cells sorting (FACS) of immune cells from the spleen and lymph nodes. No significant differences were found in body weight gain or tumor size. Metastasis was significantly reduced in the TT-treated mice compared to mock-treated mice. Cancer significantly elevated eleven cytokines. TT significantly reduced IL-1-a, MIG, IL-1b, and MIP-2 to control/vehicle levels. FACS demonstrated that TT significantly reduced specific splenic lymphocyte subsets and macrophages were significantly elevated with cancer. Human biofield therapy had no significant effect on primary tumor but produced significant effects on metastasis and immune responses in a mouse breast cancer model.

No MeSH data available.


Related in: MedlinePlus