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Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells.

Bolaños-Carrillo MA, Ventura-Gallegos JL, Saldivar-Jiménez AD, Zentella-Dehesa A, Martínez-Vázquez M - Evid Based Complement Alternat Med (2015)

Bottom Line: Methods.Conclusions.Our results showed that the mechanism of cytotoxicity displayed by peniocerol and macdougallin is related to cell cycle arrest and apoptosis in both cell lines.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidad Nacional Autónoma de México, 04510 México, DF, Mexico.

ABSTRACT
Objective. To explore the effect of peniocerol and macdougallin on HCT-15 and MCF-7 cells proliferation, cell cycle, apoptosis, and PARP cleavage. Methods. HCT-15 and MCF-7 cells were treated with various concentrations of peniocerol and macdougallin (10-80 μM) during 24 or 48 h. Crystal Violet Assay was used to evaluate the inhibition effect. Cell cycle regulation was examined by a propidium iodide method. Cell apoptosis was detected through both Annexin-V FLUOS/PI double-labeled cytometry assays and Western blot was applied to assess PARP cleavage. Results. Peniocerol and macdougallin induced growth inhibition and apoptosis in vitro in a time- and dose-dependent manner. Moreover, peniocerol and macdougallin induced arrest of cell cycle-dependent manner and increased the proportion of cells in G0/G1 phase. PARP cleavage in HCT-15 and MCF-7 cells was induced by treatment with peniocerol and macdougallin after 36 hours. Conclusions. Our results showed that the mechanism of cytotoxicity displayed by peniocerol and macdougallin is related to cell cycle arrest and apoptosis in both cell lines. This is a significant observation because it helps to understand the way some oxysterols isolated from Myrtillocactus geometrizans develop their biological activities against cancer cells.

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Related in: MedlinePlus

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Mentions: In recent years, several studies performed in vitro have shown that the cytotoxic effects of several sterols are mediated by apoptosis [16, 17]. In agreement with these observations, the Annexin V/Propidium Iodide double-staining results presented here showed that cell death induced by 3 and 4 (Figure 5) is also due to apoptosis (Tables 2 and 3).


Effect of Sterols Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells.

Bolaños-Carrillo MA, Ventura-Gallegos JL, Saldivar-Jiménez AD, Zentella-Dehesa A, Martínez-Vázquez M - Evid Based Complement Alternat Med (2015)

© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465765&req=5

Mentions: In recent years, several studies performed in vitro have shown that the cytotoxic effects of several sterols are mediated by apoptosis [16, 17]. In agreement with these observations, the Annexin V/Propidium Iodide double-staining results presented here showed that cell death induced by 3 and 4 (Figure 5) is also due to apoptosis (Tables 2 and 3).

Bottom Line: Methods.Conclusions.Our results showed that the mechanism of cytotoxicity displayed by peniocerol and macdougallin is related to cell cycle arrest and apoptosis in both cell lines.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Química, Universidad Nacional Autónoma de México, 04510 México, DF, Mexico.

ABSTRACT
Objective. To explore the effect of peniocerol and macdougallin on HCT-15 and MCF-7 cells proliferation, cell cycle, apoptosis, and PARP cleavage. Methods. HCT-15 and MCF-7 cells were treated with various concentrations of peniocerol and macdougallin (10-80 μM) during 24 or 48 h. Crystal Violet Assay was used to evaluate the inhibition effect. Cell cycle regulation was examined by a propidium iodide method. Cell apoptosis was detected through both Annexin-V FLUOS/PI double-labeled cytometry assays and Western blot was applied to assess PARP cleavage. Results. Peniocerol and macdougallin induced growth inhibition and apoptosis in vitro in a time- and dose-dependent manner. Moreover, peniocerol and macdougallin induced arrest of cell cycle-dependent manner and increased the proportion of cells in G0/G1 phase. PARP cleavage in HCT-15 and MCF-7 cells was induced by treatment with peniocerol and macdougallin after 36 hours. Conclusions. Our results showed that the mechanism of cytotoxicity displayed by peniocerol and macdougallin is related to cell cycle arrest and apoptosis in both cell lines. This is a significant observation because it helps to understand the way some oxysterols isolated from Myrtillocactus geometrizans develop their biological activities against cancer cells.

No MeSH data available.


Related in: MedlinePlus