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Changes in Sperm Motility and Capacitation Induce Chromosomal Aberration of the Bovine Embryo following Intracytoplasmic Sperm Injection.

Kato Y, Nagao Y - PLoS ONE (2015)

Bottom Line: Secondly, we treated sperm with carbonyl cyanide m-chlorophenyl hydrazine (CCCP), a chemical known to uncouple cell respiration within the mitochondria, and investigated the effect of this treatment upon blastocyst formation and chromosomal integrity at the blastocyst stage.In conclusion, these findings suggest that capacitated sperm exhibiting activated motility induced chromosomal aberration during development to the blastocyst stage following ICSI.The injection of sperm exhibiting normal motility, or activated sperm in which mitochondrial activity had been reduced, improved the quality of ICSI-derived embryos.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan; University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan.

ABSTRACT
Intracytoplasmic sperm injection (ICSI) has become the method of choice to treat human male infertility. One of the outstanding problems associated with this technique is our current lack of knowledge concerning the effect of sperm capacitation and motility upon the subsequent development of oocytes following ICSI. In the present study, we first examined the capacitation state of sperm exhibiting normal motility, along with sperm that had been activated, and examined the effect of reactive oxygen species (ROS) produced by these sperm types upon embryogenesis following bovine in vitro fertilization (IVF) and ICSI. Data showed that activated sperm reduced the chromosomal integrity of IVF/ICSI embryos at the blastocyst stage, while capacitated sperm produced ROS in capacitation media. Secondly, we treated sperm with carbonyl cyanide m-chlorophenyl hydrazine (CCCP), a chemical known to uncouple cell respiration within the mitochondria, and investigated the effect of this treatment upon blastocyst formation and chromosomal integrity at the blastocyst stage. Activated sperm in which the mitochondria had been treated with CCCP reduced levels of chromosomal aberration at the blastocyst stage following ICSI, by reducing mitochondrial activity in activated sperm. In conclusion, these findings suggest that capacitated sperm exhibiting activated motility induced chromosomal aberration during development to the blastocyst stage following ICSI. The injection of sperm exhibiting normal motility, or activated sperm in which mitochondrial activity had been reduced, improved the quality of ICSI-derived embryos. Therefore, the selection of sperm exhibiting progressive motility may not always be better for early embryo development and fetal growth following human ICSI, and that the use of a bovine model may contribute to a deeper understanding of sperm selection for human ICSI embryo development.

No MeSH data available.


Related in: MedlinePlus

Sperm motility patterns of bovine spermatozoa cultured in non-capacitation media and capacitation media.Top left; normal motile sperm, right; sperm exhibiting activated motility, bottom; hyper-activated motility.
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pone.0129285.g001: Sperm motility patterns of bovine spermatozoa cultured in non-capacitation media and capacitation media.Top left; normal motile sperm, right; sperm exhibiting activated motility, bottom; hyper-activated motility.

Mentions: Sperm motility was categorized according to motility patterns as previously described [15, 26]. Over 100 sperm were examined in PBS containing 10% polyvinylpyrrolidone (PVP) solution in order to reduce and consider sperm motility on an interference contrast microscope. The ratios (%) of normal motility, activated, and hyper-activated motility sperm were determined as follows. Normal motile sperm exhibited a motile tail (Fig 1, Top left). Following the activation of motility, sperm head and flagella beat in an almost symmetrical fashion, propelling the sperm in almost linear trajectories [27–29] (Fig 1, Top right). Hyper-activated motility was characterized by asymmetrical beating of the flagellum resulting in a circular or vigorous ‘‘figure of 8” trajectory [27–29] (Fig 1, below). The proportion (%) of motile sperm refers to the number of motile sperm divided by the number of total sperm, including all motile and immotile sperm in each media, respectively. The proportion (%) of activated sperm refers to the number of such sperm divided by the number of motile sperm in each media, respectively. Finally, the proportion (%) of hyper-activated motility sperm refers to the number of such sperm divided by the number of motile sperm in each media, respectively.


Changes in Sperm Motility and Capacitation Induce Chromosomal Aberration of the Bovine Embryo following Intracytoplasmic Sperm Injection.

Kato Y, Nagao Y - PLoS ONE (2015)

Sperm motility patterns of bovine spermatozoa cultured in non-capacitation media and capacitation media.Top left; normal motile sperm, right; sperm exhibiting activated motility, bottom; hyper-activated motility.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465702&req=5

pone.0129285.g001: Sperm motility patterns of bovine spermatozoa cultured in non-capacitation media and capacitation media.Top left; normal motile sperm, right; sperm exhibiting activated motility, bottom; hyper-activated motility.
Mentions: Sperm motility was categorized according to motility patterns as previously described [15, 26]. Over 100 sperm were examined in PBS containing 10% polyvinylpyrrolidone (PVP) solution in order to reduce and consider sperm motility on an interference contrast microscope. The ratios (%) of normal motility, activated, and hyper-activated motility sperm were determined as follows. Normal motile sperm exhibited a motile tail (Fig 1, Top left). Following the activation of motility, sperm head and flagella beat in an almost symmetrical fashion, propelling the sperm in almost linear trajectories [27–29] (Fig 1, Top right). Hyper-activated motility was characterized by asymmetrical beating of the flagellum resulting in a circular or vigorous ‘‘figure of 8” trajectory [27–29] (Fig 1, below). The proportion (%) of motile sperm refers to the number of motile sperm divided by the number of total sperm, including all motile and immotile sperm in each media, respectively. The proportion (%) of activated sperm refers to the number of such sperm divided by the number of motile sperm in each media, respectively. Finally, the proportion (%) of hyper-activated motility sperm refers to the number of such sperm divided by the number of motile sperm in each media, respectively.

Bottom Line: Secondly, we treated sperm with carbonyl cyanide m-chlorophenyl hydrazine (CCCP), a chemical known to uncouple cell respiration within the mitochondria, and investigated the effect of this treatment upon blastocyst formation and chromosomal integrity at the blastocyst stage.In conclusion, these findings suggest that capacitated sperm exhibiting activated motility induced chromosomal aberration during development to the blastocyst stage following ICSI.The injection of sperm exhibiting normal motility, or activated sperm in which mitochondrial activity had been reduced, improved the quality of ICSI-derived embryos.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan; University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan.

ABSTRACT
Intracytoplasmic sperm injection (ICSI) has become the method of choice to treat human male infertility. One of the outstanding problems associated with this technique is our current lack of knowledge concerning the effect of sperm capacitation and motility upon the subsequent development of oocytes following ICSI. In the present study, we first examined the capacitation state of sperm exhibiting normal motility, along with sperm that had been activated, and examined the effect of reactive oxygen species (ROS) produced by these sperm types upon embryogenesis following bovine in vitro fertilization (IVF) and ICSI. Data showed that activated sperm reduced the chromosomal integrity of IVF/ICSI embryos at the blastocyst stage, while capacitated sperm produced ROS in capacitation media. Secondly, we treated sperm with carbonyl cyanide m-chlorophenyl hydrazine (CCCP), a chemical known to uncouple cell respiration within the mitochondria, and investigated the effect of this treatment upon blastocyst formation and chromosomal integrity at the blastocyst stage. Activated sperm in which the mitochondria had been treated with CCCP reduced levels of chromosomal aberration at the blastocyst stage following ICSI, by reducing mitochondrial activity in activated sperm. In conclusion, these findings suggest that capacitated sperm exhibiting activated motility induced chromosomal aberration during development to the blastocyst stage following ICSI. The injection of sperm exhibiting normal motility, or activated sperm in which mitochondrial activity had been reduced, improved the quality of ICSI-derived embryos. Therefore, the selection of sperm exhibiting progressive motility may not always be better for early embryo development and fetal growth following human ICSI, and that the use of a bovine model may contribute to a deeper understanding of sperm selection for human ICSI embryo development.

No MeSH data available.


Related in: MedlinePlus