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Effects of Atrazine on the Development of Neural System of Zebrafish, Danio rerio.

Wang H, Mu S, Zhang F, Wang H, Liu H, Zhang H, Kang X - Biomed Res Int (2015)

Bottom Line: This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened.Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired.These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.

ABSTRACT
By comparative analysis of histomorphology and AChE activity, the changes of physiological and biochemical parameters were determined in zebrafish embryos and larvae dealt with atrazine (ATR) at different concentrations (0.0001, 0.001, 0.01, 0.1, and 1 mg/L). This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened. Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired. Conversely, the AChE activity in zebrafish embryos which was treated with ATR was upregulated. A parallel phenomenon showed that embryonic primary sensory neurons (Rohon-Beard cells), principally expressing AChE in embryos, survived the physiological apoptosis. These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.

No MeSH data available.


Related in: MedlinePlus

ATR bidirectionally regulated AChE activity of embryos and larvae exposed to ATR at early embryonic stages. This figure was a trend chart made by the data in Table 1. Before hatching (36 and 48 hpf), the high concentration of ATR could increase the activity of AChE; to the contrary, the activity of AChE was inhibited by the high concentration of ATR after incubation. Figure in the ATR concentration gradient (mg/L): C0, 0 (DMSO); C1, 0.0001; C2, 0.001; C3, 0.01; C4, 0.1; and C5, 1. 30 zebrafish embryos were used for each experimental group (24 hpf, 36 hpf, 48 hpf, and 72 hpf).
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fig4: ATR bidirectionally regulated AChE activity of embryos and larvae exposed to ATR at early embryonic stages. This figure was a trend chart made by the data in Table 1. Before hatching (36 and 48 hpf), the high concentration of ATR could increase the activity of AChE; to the contrary, the activity of AChE was inhibited by the high concentration of ATR after incubation. Figure in the ATR concentration gradient (mg/L): C0, 0 (DMSO); C1, 0.0001; C2, 0.001; C3, 0.01; C4, 0.1; and C5, 1. 30 zebrafish embryos were used for each experimental group (24 hpf, 36 hpf, 48 hpf, and 72 hpf).

Mentions: Relationship between AChE activity and the concentration of the ATR was mapped according to the results of measurement (Figure 4). The AChE activity showed a rising trend with the increasing concentration of ATR before hatching. At 24 hpf, the activity of enzyme in test groups and control group remained in basal level. There was no obvious difference between them. At 36 hpf the activity of enzyme in all groups dealt with ATR was higher than the control's, which increased significantly. The curve of activity of enzyme at 48 hpf showed a slightly rising trend; the activity rose with the increase of concentration of ATR. After hatching (72 hpf), the curve of AChE activity showed downward trend with the increase of concentration of ATR; the inhibition to all the groups treated with ATR was significant; enzyme activity had the greatest reduction with 1 mg ATR/L (25%).


Effects of Atrazine on the Development of Neural System of Zebrafish, Danio rerio.

Wang H, Mu S, Zhang F, Wang H, Liu H, Zhang H, Kang X - Biomed Res Int (2015)

ATR bidirectionally regulated AChE activity of embryos and larvae exposed to ATR at early embryonic stages. This figure was a trend chart made by the data in Table 1. Before hatching (36 and 48 hpf), the high concentration of ATR could increase the activity of AChE; to the contrary, the activity of AChE was inhibited by the high concentration of ATR after incubation. Figure in the ATR concentration gradient (mg/L): C0, 0 (DMSO); C1, 0.0001; C2, 0.001; C3, 0.01; C4, 0.1; and C5, 1. 30 zebrafish embryos were used for each experimental group (24 hpf, 36 hpf, 48 hpf, and 72 hpf).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4465686&req=5

fig4: ATR bidirectionally regulated AChE activity of embryos and larvae exposed to ATR at early embryonic stages. This figure was a trend chart made by the data in Table 1. Before hatching (36 and 48 hpf), the high concentration of ATR could increase the activity of AChE; to the contrary, the activity of AChE was inhibited by the high concentration of ATR after incubation. Figure in the ATR concentration gradient (mg/L): C0, 0 (DMSO); C1, 0.0001; C2, 0.001; C3, 0.01; C4, 0.1; and C5, 1. 30 zebrafish embryos were used for each experimental group (24 hpf, 36 hpf, 48 hpf, and 72 hpf).
Mentions: Relationship between AChE activity and the concentration of the ATR was mapped according to the results of measurement (Figure 4). The AChE activity showed a rising trend with the increasing concentration of ATR before hatching. At 24 hpf, the activity of enzyme in test groups and control group remained in basal level. There was no obvious difference between them. At 36 hpf the activity of enzyme in all groups dealt with ATR was higher than the control's, which increased significantly. The curve of activity of enzyme at 48 hpf showed a slightly rising trend; the activity rose with the increase of concentration of ATR. After hatching (72 hpf), the curve of AChE activity showed downward trend with the increase of concentration of ATR; the inhibition to all the groups treated with ATR was significant; enzyme activity had the greatest reduction with 1 mg ATR/L (25%).

Bottom Line: This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened.Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired.These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Hebei University, Wusi East Road 180, Baoding 071002, China.

ABSTRACT
By comparative analysis of histomorphology and AChE activity, the changes of physiological and biochemical parameters were determined in zebrafish embryos and larvae dealt with atrazine (ATR) at different concentrations (0.0001, 0.001, 0.01, 0.1, and 1 mg/L). This study showed that the development of the sarcomere and the arrangement of white muscle myofibers were affected by ATR significantly and the length of sarcomere shortened. Further analysis of the results showed that the AChE activity in juvenile fish which was treated with ATR was downregulated, which can indicate that the innervation efficiency to the muscle was impaired. Conversely, the AChE activity in zebrafish embryos which was treated with ATR was upregulated. A parallel phenomenon showed that embryonic primary sensory neurons (Rohon-Beard cells), principally expressing AChE in embryos, survived the physiological apoptosis. These phenomena demonstrated that the motor integration ability of the zebrafish was damaged by ATR which can disturb the development of sensory neurons and sarcomere and the innervations of muscle.

No MeSH data available.


Related in: MedlinePlus