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Comparative Analysis of Apicoplast-Targeted Protein Extension Lengths in Apicomplexan Parasites.

Seliverstov AV, Zverkov OA, Istomina SN, Pirogov SA, Kitsis PS - Biomed Res Int (2015)

Bottom Line: More specifically, we provide a comparison of extension lengths of orthologous apicoplast-targeted proteins in apicomplexan parasites.We focus on results obtained for the model species T. gondii, Neospora caninum, and Plasmodium falciparum.Extensions in P. falciparum less than 87 residues in size are longer than the corresponding extensions in N. caninum and, reversely, are shorter if they exceed 88 residues.

View Article: PubMed Central - PubMed

Affiliation: Institute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute), Bolshoy Karetny Pereulok 19, Moscow 127994, Russia.

ABSTRACT
In general, the mechanism of protein translocation through the apicoplast membrane requires a specific extension of a functionally important region of the apicoplast-targeted proteins. The corresponding signal peptides were detected in many apicomplexans but not in the majority of apicoplast-targeted proteins in Toxoplasma gondii. In T. gondii signal peptides are either much diverged or their extension region is processed, which in either case makes the situation different from other studied apicomplexans. We propose a statistic method to compare extensions of the functionally important regions of apicoplast-targeted proteins. More specifically, we provide a comparison of extension lengths of orthologous apicoplast-targeted proteins in apicomplexan parasites. We focus on results obtained for the model species T. gondii, Neospora caninum, and Plasmodium falciparum. With our method, cross species comparisons demonstrate that, in average, apicoplast-targeted protein extensions in T. gondii are 1.5-fold longer than in N. caninum and 2-fold longer than in P. falciparum. Extensions in P. falciparum less than 87 residues in size are longer than the corresponding extensions in N. caninum and, reversely, are shorter if they exceed 88 residues.

No MeSH data available.


Related in: MedlinePlus

Plot of protein length extensions in T. gondii ME49 versus P. falciparum 3D7 relative to their orthologs in Synechocystis sp. PCC 6803. Differences in lengths between sporozoan and cyanobacterial orthologous proteins are plotted as follows: T-S on the x-axis, P-S on the y-axis.
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fig2: Plot of protein length extensions in T. gondii ME49 versus P. falciparum 3D7 relative to their orthologs in Synechocystis sp. PCC 6803. Differences in lengths between sporozoan and cyanobacterial orthologous proteins are plotted as follows: T-S on the x-axis, P-S on the y-axis.

Mentions: Protein extensions in T. gondii are on average 2-fold longer compared to the corresponding extensions in P. falciparum, with high confidence (Figure 2).


Comparative Analysis of Apicoplast-Targeted Protein Extension Lengths in Apicomplexan Parasites.

Seliverstov AV, Zverkov OA, Istomina SN, Pirogov SA, Kitsis PS - Biomed Res Int (2015)

Plot of protein length extensions in T. gondii ME49 versus P. falciparum 3D7 relative to their orthologs in Synechocystis sp. PCC 6803. Differences in lengths between sporozoan and cyanobacterial orthologous proteins are plotted as follows: T-S on the x-axis, P-S on the y-axis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465681&req=5

fig2: Plot of protein length extensions in T. gondii ME49 versus P. falciparum 3D7 relative to their orthologs in Synechocystis sp. PCC 6803. Differences in lengths between sporozoan and cyanobacterial orthologous proteins are plotted as follows: T-S on the x-axis, P-S on the y-axis.
Mentions: Protein extensions in T. gondii are on average 2-fold longer compared to the corresponding extensions in P. falciparum, with high confidence (Figure 2).

Bottom Line: More specifically, we provide a comparison of extension lengths of orthologous apicoplast-targeted proteins in apicomplexan parasites.We focus on results obtained for the model species T. gondii, Neospora caninum, and Plasmodium falciparum.Extensions in P. falciparum less than 87 residues in size are longer than the corresponding extensions in N. caninum and, reversely, are shorter if they exceed 88 residues.

View Article: PubMed Central - PubMed

Affiliation: Institute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute), Bolshoy Karetny Pereulok 19, Moscow 127994, Russia.

ABSTRACT
In general, the mechanism of protein translocation through the apicoplast membrane requires a specific extension of a functionally important region of the apicoplast-targeted proteins. The corresponding signal peptides were detected in many apicomplexans but not in the majority of apicoplast-targeted proteins in Toxoplasma gondii. In T. gondii signal peptides are either much diverged or their extension region is processed, which in either case makes the situation different from other studied apicomplexans. We propose a statistic method to compare extensions of the functionally important regions of apicoplast-targeted proteins. More specifically, we provide a comparison of extension lengths of orthologous apicoplast-targeted proteins in apicomplexan parasites. We focus on results obtained for the model species T. gondii, Neospora caninum, and Plasmodium falciparum. With our method, cross species comparisons demonstrate that, in average, apicoplast-targeted protein extensions in T. gondii are 1.5-fold longer than in N. caninum and 2-fold longer than in P. falciparum. Extensions in P. falciparum less than 87 residues in size are longer than the corresponding extensions in N. caninum and, reversely, are shorter if they exceed 88 residues.

No MeSH data available.


Related in: MedlinePlus