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Preoperative Prediction of Ki-67 Labeling Index By Three-dimensional CT Image Parameters for Differential Diagnosis Of Ground-Glass Opacity (GGO).

Peng M, Peng F, Zhang C, Wang Q, Li Z, Hu H, Liu S, Xu B, Zhu W, Han Y, Lin Q - PLoS ONE (2015)

Bottom Line: Diameter, TV, MAX, AVG and STD increased along with PIA, MIA and IAC significantly and consecutively.Diameter, TV, MAX, AVG and STD could discriminate pathologic categories of GGO nodules significantly.Ki-67 LI of early lung adenocarcinoma presenting GGO can be predicted by radiologic parameters based on 3D CT for differential diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital Affiliated to The Shanghai Jiao Tong University School Of Medicine, Shanghai, China.

ABSTRACT
The aim of this study was to predict Ki-67 labeling index (LI) preoperatively by three-dimensional (3D) CT image parameters for pathologic assessment of GGO nodules. Diameter, total volume (TV), the maximum CT number (MAX), average CT number (AVG) and standard deviation of CT number within the whole GGO nodule (STD) were measured by 3D CT workstation. By detection of immunohistochemistry and Image Software Pro Plus 6.0, different Ki-67 LI were measured and statistically analyzed among preinvasive adenocarcinoma (PIA), minimally invasive adenocarcinoma (MIA) and invasive adenocarcinoma (IAC). Receiver operating characteristic (ROC) curve, Spearman correlation analysis and multiple linear regression analysis with cross-validation were performed to further research a quantitative correlation between Ki-67 labeling index and radiological parameters. Diameter, TV, MAX, AVG and STD increased along with PIA, MIA and IAC significantly and consecutively. In the multiple linear regression model by a stepwise way, we obtained an equation: prediction of Ki-67 LI=0.022*STD+0.001* TV+2.137 (R=0.595, R's square=0.354, p<0.001), which can predict Ki-67 LI as a proliferative marker preoperatively. Diameter, TV, MAX, AVG and STD could discriminate pathologic categories of GGO nodules significantly. Ki-67 LI of early lung adenocarcinoma presenting GGO can be predicted by radiologic parameters based on 3D CT for differential diagnosis.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of ground-glass opacity (GGO) (hematoxylin-eosin stain, original magnification, ×400).(A) A case of atypical adenomatous hyperplasia (AAH) with a well-defined boundary and pulmonary mesenchyme. (B) A case of adenocarcinoma in situ (AIS) with growth restricted to preexisting alveolar walls. No foci of invasion or scarring are seen. (C) A case of minimally invasive adenocarcinoma (MIA) consisting primarily of lepidic growth with a small area of acinar invasion.
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pone.0129206.g002: Photomicrographs of ground-glass opacity (GGO) (hematoxylin-eosin stain, original magnification, ×400).(A) A case of atypical adenomatous hyperplasia (AAH) with a well-defined boundary and pulmonary mesenchyme. (B) A case of adenocarcinoma in situ (AIS) with growth restricted to preexisting alveolar walls. No foci of invasion or scarring are seen. (C) A case of minimally invasive adenocarcinoma (MIA) consisting primarily of lepidic growth with a small area of acinar invasion.

Mentions: The surgically resected specimens were routinely fixed in 10% formalin, and processed into paraffin blocks for pathologic examination over the entire volumes of GGO nodules. Tissue sections were cut at 4μm thickness, including the largest cut surface of the tumor, and stained with both hematoxylin and eosin (H&E). Pathologic diagnoses were made by two experienced lung pathologists. According to the new IASLC/ATS/ERS classification,[6] all of GGO nodules were diagnosed as AAH, AIS, MIA and IAC. (Fig 2)


Preoperative Prediction of Ki-67 Labeling Index By Three-dimensional CT Image Parameters for Differential Diagnosis Of Ground-Glass Opacity (GGO).

Peng M, Peng F, Zhang C, Wang Q, Li Z, Hu H, Liu S, Xu B, Zhu W, Han Y, Lin Q - PLoS ONE (2015)

Photomicrographs of ground-glass opacity (GGO) (hematoxylin-eosin stain, original magnification, ×400).(A) A case of atypical adenomatous hyperplasia (AAH) with a well-defined boundary and pulmonary mesenchyme. (B) A case of adenocarcinoma in situ (AIS) with growth restricted to preexisting alveolar walls. No foci of invasion or scarring are seen. (C) A case of minimally invasive adenocarcinoma (MIA) consisting primarily of lepidic growth with a small area of acinar invasion.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465676&req=5

pone.0129206.g002: Photomicrographs of ground-glass opacity (GGO) (hematoxylin-eosin stain, original magnification, ×400).(A) A case of atypical adenomatous hyperplasia (AAH) with a well-defined boundary and pulmonary mesenchyme. (B) A case of adenocarcinoma in situ (AIS) with growth restricted to preexisting alveolar walls. No foci of invasion or scarring are seen. (C) A case of minimally invasive adenocarcinoma (MIA) consisting primarily of lepidic growth with a small area of acinar invasion.
Mentions: The surgically resected specimens were routinely fixed in 10% formalin, and processed into paraffin blocks for pathologic examination over the entire volumes of GGO nodules. Tissue sections were cut at 4μm thickness, including the largest cut surface of the tumor, and stained with both hematoxylin and eosin (H&E). Pathologic diagnoses were made by two experienced lung pathologists. According to the new IASLC/ATS/ERS classification,[6] all of GGO nodules were diagnosed as AAH, AIS, MIA and IAC. (Fig 2)

Bottom Line: Diameter, TV, MAX, AVG and STD increased along with PIA, MIA and IAC significantly and consecutively.Diameter, TV, MAX, AVG and STD could discriminate pathologic categories of GGO nodules significantly.Ki-67 LI of early lung adenocarcinoma presenting GGO can be predicted by radiologic parameters based on 3D CT for differential diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, Shanghai First People's Hospital Affiliated to The Shanghai Jiao Tong University School Of Medicine, Shanghai, China.

ABSTRACT
The aim of this study was to predict Ki-67 labeling index (LI) preoperatively by three-dimensional (3D) CT image parameters for pathologic assessment of GGO nodules. Diameter, total volume (TV), the maximum CT number (MAX), average CT number (AVG) and standard deviation of CT number within the whole GGO nodule (STD) were measured by 3D CT workstation. By detection of immunohistochemistry and Image Software Pro Plus 6.0, different Ki-67 LI were measured and statistically analyzed among preinvasive adenocarcinoma (PIA), minimally invasive adenocarcinoma (MIA) and invasive adenocarcinoma (IAC). Receiver operating characteristic (ROC) curve, Spearman correlation analysis and multiple linear regression analysis with cross-validation were performed to further research a quantitative correlation between Ki-67 labeling index and radiological parameters. Diameter, TV, MAX, AVG and STD increased along with PIA, MIA and IAC significantly and consecutively. In the multiple linear regression model by a stepwise way, we obtained an equation: prediction of Ki-67 LI=0.022*STD+0.001* TV+2.137 (R=0.595, R's square=0.354, p<0.001), which can predict Ki-67 LI as a proliferative marker preoperatively. Diameter, TV, MAX, AVG and STD could discriminate pathologic categories of GGO nodules significantly. Ki-67 LI of early lung adenocarcinoma presenting GGO can be predicted by radiologic parameters based on 3D CT for differential diagnosis.

No MeSH data available.


Related in: MedlinePlus