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Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.

Meczekalski B, Nawrot R, Nowak W, Czyzyk A, Kedzia H, Gozdzicka-Jozefiak A - J. Endocrinol. Invest. (2015)

Bottom Line: In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens.In one case, we found a mutation of the ZP4 gene.No correlations were found between the ZP4 expression level and biochemical or clinical indices.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Endocrinology, Poznan University of Medical Sciences, Polna 33, 60-535, Poznan, Poland. blazejmeczekalski@yahoo.com.

ABSTRACT

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder of unknown pathology, involving reproductive and metabolic abnormalities. Oocyte-specific genes are a group of genes expressed exclusively in ovarian tissue; therefore, they can play an important role in ovarian pathologies such as PCOS. The zona pellucida 4 (ZP4) gene encodes glycoprotein which is a part of the extracellular matrix of oocyte.

Materials and methods: We analyzed 87 patients with PCOS, which were divided into four groups depending on their phenotype. In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens. The ovarian tissue samples were used to perform a real-time polymerase chain reaction and immunohistochemical staining using anti-ZP4 monoclonal antibodies. The ZP4 gene was sequenced from peripheral lymphocytes.

Results: The expression of ZP4 was present in early antral follicles and was stronger in mature follicles. The subgroup of patients with eumenorrhea and without hyperandrogenism presented the highest expression of ZP4 in ovarian tissue. In one case, we found a mutation of the ZP4 gene. No correlations were found between the ZP4 expression level and biochemical or clinical indices.

Conclusions: Data from this and animal studies suggest a possible relationship between androgens and ZP4 expression. ZP4 expression is highest among patients with PCOS and a regular cycle, and this is a consequence of the presence of mature follicles in this group. In some patients with PCOS and infertility, ZP4 mutation can be found.

No MeSH data available.


Related in: MedlinePlus

Mean ZP4 expression level in studied groups (vertical‚ÄĒnumber of copies)
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Related In: Results  -  Collection


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Fig1: Mean ZP4 expression level in studied groups (vertical‚ÄĒnumber of copies)

Mentions: In the OM¬†+¬†HA¬†+¬†US group, the mean Zp4 expression level was estimated as 373.212¬†¬Ī¬†341.75 number of copies, whereas in the HA¬†+¬†US group, it was 852.0¬†¬Ī¬†742.0, in the OM¬†+¬†US group, it was 636.7¬†¬Ī¬†516.51 and in the OM¬†+¬†HA group, it was 124.1¬†¬Ī¬†137.8. Considering all the groups with polycystic ovaries (I¬†+¬†II¬†+¬†III) together, the mean expression level in the RT-PCR was 620.6¬†¬Ī¬†3780.5 numbers of copies (Fig.¬†1). The statistical analysis revealed that the differences between all the groups were significant p¬†<¬†0.0001 (ANOVA test). Post hoc analyses revealed that the difference was significant between groups OM¬†+¬†HA¬†+¬†US and HA¬†+¬†US; HA¬†+¬†US and OM¬†+¬†HA; and OM¬†+¬†US and OM¬†+¬†HA.Fig.¬†1


Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.

Meczekalski B, Nawrot R, Nowak W, Czyzyk A, Kedzia H, Gozdzicka-Jozefiak A - J. Endocrinol. Invest. (2015)

Mean ZP4 expression level in studied groups (vertical‚ÄĒnumber of copies)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465666&req=5

Fig1: Mean ZP4 expression level in studied groups (vertical‚ÄĒnumber of copies)
Mentions: In the OM¬†+¬†HA¬†+¬†US group, the mean Zp4 expression level was estimated as 373.212¬†¬Ī¬†341.75 number of copies, whereas in the HA¬†+¬†US group, it was 852.0¬†¬Ī¬†742.0, in the OM¬†+¬†US group, it was 636.7¬†¬Ī¬†516.51 and in the OM¬†+¬†HA group, it was 124.1¬†¬Ī¬†137.8. Considering all the groups with polycystic ovaries (I¬†+¬†II¬†+¬†III) together, the mean expression level in the RT-PCR was 620.6¬†¬Ī¬†3780.5 numbers of copies (Fig.¬†1). The statistical analysis revealed that the differences between all the groups were significant p¬†<¬†0.0001 (ANOVA test). Post hoc analyses revealed that the difference was significant between groups OM¬†+¬†HA¬†+¬†US and HA¬†+¬†US; HA¬†+¬†US and OM¬†+¬†HA; and OM¬†+¬†US and OM¬†+¬†HA.Fig.¬†1

Bottom Line: In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens.In one case, we found a mutation of the ZP4 gene.No correlations were found between the ZP4 expression level and biochemical or clinical indices.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Endocrinology, Poznan University of Medical Sciences, Polna 33, 60-535, Poznan, Poland. blazejmeczekalski@yahoo.com.

ABSTRACT

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder of unknown pathology, involving reproductive and metabolic abnormalities. Oocyte-specific genes are a group of genes expressed exclusively in ovarian tissue; therefore, they can play an important role in ovarian pathologies such as PCOS. The zona pellucida 4 (ZP4) gene encodes glycoprotein which is a part of the extracellular matrix of oocyte.

Materials and methods: We analyzed 87 patients with PCOS, which were divided into four groups depending on their phenotype. In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens. The ovarian tissue samples were used to perform a real-time polymerase chain reaction and immunohistochemical staining using anti-ZP4 monoclonal antibodies. The ZP4 gene was sequenced from peripheral lymphocytes.

Results: The expression of ZP4 was present in early antral follicles and was stronger in mature follicles. The subgroup of patients with eumenorrhea and without hyperandrogenism presented the highest expression of ZP4 in ovarian tissue. In one case, we found a mutation of the ZP4 gene. No correlations were found between the ZP4 expression level and biochemical or clinical indices.

Conclusions: Data from this and animal studies suggest a possible relationship between androgens and ZP4 expression. ZP4 expression is highest among patients with PCOS and a regular cycle, and this is a consequence of the presence of mature follicles in this group. In some patients with PCOS and infertility, ZP4 mutation can be found.

No MeSH data available.


Related in: MedlinePlus