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A Novel Injectable Magnesium/Calcium Sulfate Hemihydrate Composite Cement for Bone Regeneration.

Zhang S, Yang K, Cui F, Jiang Y, E L, Xu B, Liu H - Biomed Res Int (2015)

Bottom Line: A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Center of Stomatology, China-Japan Friendship Hospital, Beijing 100029, China ; Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.

ABSTRACT

Objective: . A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.

Methods: Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated. Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated.

Results: Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property (p < 0.05). Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking (p < 0.05). Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs (p < 0.05). Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).

Conclusions: The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Quantitative analysis of BMD and BAF based on a dual energy X-ray absorptiometry system. (a) The local BMD after implantation for 4 and 12 weeks; (b) the BAF for 1 day, 4 weeks, and 12 weeks. ∗ represents significant differences between Mg/CSH composite specimens and pure CSH (p < 0.05). The bars on the graphs are standard deviations. BMD: bone mineral density; BAF: new bone area fraction; Mg: magnesium; CSH: calcium sulfate hemihydrate.
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fig9: Quantitative analysis of BMD and BAF based on a dual energy X-ray absorptiometry system. (a) The local BMD after implantation for 4 and 12 weeks; (b) the BAF for 1 day, 4 weeks, and 12 weeks. ∗ represents significant differences between Mg/CSH composite specimens and pure CSH (p < 0.05). The bars on the graphs are standard deviations. BMD: bone mineral density; BAF: new bone area fraction; Mg: magnesium; CSH: calcium sulfate hemihydrate.

Mentions: To quantify the calcification of repaired tibia, BMDs of all animals were measured on DXA 4 and 12 weeks after operation. The Mg/CSH grafts showed higher (p < 0.05) BMD values than CSH and control groups at both 4 and 12 weeks. However, there was no marked variation (p > 0.05) between 10% and 20% Mg/CSH grafts (Figure 9(a)). Meanwhile, BAF was applied to evaluate the newly formed bone after surgery for 1 d and 4 and 8 weeks. The BAFs of Mg/CSH composite graft were significantly higher (p < 0.05) than that of CSH at both 4 and 8 weeks (Figure 9(b)).


A Novel Injectable Magnesium/Calcium Sulfate Hemihydrate Composite Cement for Bone Regeneration.

Zhang S, Yang K, Cui F, Jiang Y, E L, Xu B, Liu H - Biomed Res Int (2015)

Quantitative analysis of BMD and BAF based on a dual energy X-ray absorptiometry system. (a) The local BMD after implantation for 4 and 12 weeks; (b) the BAF for 1 day, 4 weeks, and 12 weeks. ∗ represents significant differences between Mg/CSH composite specimens and pure CSH (p < 0.05). The bars on the graphs are standard deviations. BMD: bone mineral density; BAF: new bone area fraction; Mg: magnesium; CSH: calcium sulfate hemihydrate.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465663&req=5

fig9: Quantitative analysis of BMD and BAF based on a dual energy X-ray absorptiometry system. (a) The local BMD after implantation for 4 and 12 weeks; (b) the BAF for 1 day, 4 weeks, and 12 weeks. ∗ represents significant differences between Mg/CSH composite specimens and pure CSH (p < 0.05). The bars on the graphs are standard deviations. BMD: bone mineral density; BAF: new bone area fraction; Mg: magnesium; CSH: calcium sulfate hemihydrate.
Mentions: To quantify the calcification of repaired tibia, BMDs of all animals were measured on DXA 4 and 12 weeks after operation. The Mg/CSH grafts showed higher (p < 0.05) BMD values than CSH and control groups at both 4 and 12 weeks. However, there was no marked variation (p > 0.05) between 10% and 20% Mg/CSH grafts (Figure 9(a)). Meanwhile, BAF was applied to evaluate the newly formed bone after surgery for 1 d and 4 and 8 weeks. The BAFs of Mg/CSH composite graft were significantly higher (p < 0.05) than that of CSH at both 4 and 8 weeks (Figure 9(b)).

Bottom Line: A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Center of Stomatology, China-Japan Friendship Hospital, Beijing 100029, China ; Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.

ABSTRACT

Objective: . A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.

Methods: Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated. Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated.

Results: Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property (p < 0.05). Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking (p < 0.05). Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs (p < 0.05). Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).

Conclusions: The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus