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A Novel Injectable Magnesium/Calcium Sulfate Hemihydrate Composite Cement for Bone Regeneration.

Zhang S, Yang K, Cui F, Jiang Y, E L, Xu B, Liu H - Biomed Res Int (2015)

Bottom Line: A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Center of Stomatology, China-Japan Friendship Hospital, Beijing 100029, China ; Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.

ABSTRACT

Objective: . A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.

Methods: Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated. Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated.

Results: Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property (p < 0.05). Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking (p < 0.05). Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs (p < 0.05). Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).

Conclusions: The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Degradation ratios of CSH and Mg/CSH composite specimens immersed for 2, 4, 7, 10, 14, and 21 days. (a) Simulated body fluid; (b) pH values of the environment. ∗ indicates that degradation ratios of the Mg/CSH composite specimens were significantly different from those of CSH (p < 0.05). The bars on the graphs are standard deviations. Mg: magnesium; CSH: calcium sulfate hemihydrate.
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fig5: Degradation ratios of CSH and Mg/CSH composite specimens immersed for 2, 4, 7, 10, 14, and 21 days. (a) Simulated body fluid; (b) pH values of the environment. ∗ indicates that degradation ratios of the Mg/CSH composite specimens were significantly different from those of CSH (p < 0.05). The bars on the graphs are standard deviations. Mg: magnesium; CSH: calcium sulfate hemihydrate.

Mentions: The degradation ratios of the samples were characterized by the weight loss ratios after soaking in SBF for various time periods. It can be seen that there were no significant differences (p > 0.05) in the degradation ratios among all specimens from 2 to 14 days, while the degradation ratio of 20% Mg/CSH composite was significantly higher (p < 0.05) than that of other constructs after 21 days of soaking (Figure 5(a)). During immersion in SBF, the samples led to an acid environment, causing a decrease in the pH values of SBF. However, no remarkable difference (p > 0.05) was observed among the three kinds of construct at selected time points (Figure 5(b)).


A Novel Injectable Magnesium/Calcium Sulfate Hemihydrate Composite Cement for Bone Regeneration.

Zhang S, Yang K, Cui F, Jiang Y, E L, Xu B, Liu H - Biomed Res Int (2015)

Degradation ratios of CSH and Mg/CSH composite specimens immersed for 2, 4, 7, 10, 14, and 21 days. (a) Simulated body fluid; (b) pH values of the environment. ∗ indicates that degradation ratios of the Mg/CSH composite specimens were significantly different from those of CSH (p < 0.05). The bars on the graphs are standard deviations. Mg: magnesium; CSH: calcium sulfate hemihydrate.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465663&req=5

fig5: Degradation ratios of CSH and Mg/CSH composite specimens immersed for 2, 4, 7, 10, 14, and 21 days. (a) Simulated body fluid; (b) pH values of the environment. ∗ indicates that degradation ratios of the Mg/CSH composite specimens were significantly different from those of CSH (p < 0.05). The bars on the graphs are standard deviations. Mg: magnesium; CSH: calcium sulfate hemihydrate.
Mentions: The degradation ratios of the samples were characterized by the weight loss ratios after soaking in SBF for various time periods. It can be seen that there were no significant differences (p > 0.05) in the degradation ratios among all specimens from 2 to 14 days, while the degradation ratio of 20% Mg/CSH composite was significantly higher (p < 0.05) than that of other constructs after 21 days of soaking (Figure 5(a)). During immersion in SBF, the samples led to an acid environment, causing a decrease in the pH values of SBF. However, no remarkable difference (p > 0.05) was observed among the three kinds of construct at selected time points (Figure 5(b)).

Bottom Line: A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: Center of Stomatology, China-Japan Friendship Hospital, Beijing 100029, China ; Institute of Stomatology, General Hospital of Chinese PLA, Beijing 100853, China.

ABSTRACT

Objective: . A novel injectable magnesium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties was reported here.

Methods: Composition, setting time, injectability, compressive strength, and bioactivity in simulated body fluid (SBF) of the Mg/CSH composite were evaluated. Furthermore, the cellular responses of canine bone marrow stromal cells (cBMSCs) and bone formation capacity after the implantation of Mg/CSH in tibia defects of canine were investigated.

Results: Mg/CSH possessed a prolonged setting time and markedly improved injectability and mechanical property (p < 0.05). Mg/CSH samples showed better degradability than CSH in SBF after 21 days of soaking (p < 0.05). Moreover, the degrees of cell attachment, proliferation, and capability of osteogenic differentiation on the Mg/CSH specimens were higher than those on CSH, without significant cytotoxicity and with the increased proliferation index, ALP activity, and expression levels of integrin β1 and Coll I in cBMSCs (p < 0.05). Mg/CSH enhanced the efficiency of new bone formation at the tibia defect area, including the significantly elevated bone mineral density, bone area fraction, and Coll I expression level (p < 0.05).

Conclusions: The results implied that this new injectable bone scaffold exhibited promising prospects for bone repair and had a great potential in bone tissue engineering.

No MeSH data available.


Related in: MedlinePlus