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miR-1322 Binding Sites in Paralogous and Orthologous Genes.

Niyazova R, Berillo O, Atambayeva S, Pyrkova A, Alybayeva A, Ivashchenko A - Biomed Res Int (2015)

Bottom Line: From two to 28 binding sites have arranged localization with the start position through three nucleotides of each following binding site.The nucleotide sequences of these sites in CDSs encode oligopeptides with the same and/or different amino acid sequences.These sites encode a polyglutamine oligopeptide ranging from six to 47 amino acids in length.

View Article: PubMed Central - PubMed

Affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.

ABSTRACT
We searched for 2,563 microRNA (miRNA) binding sites in 17,494 mRNA sequences of human genes. miR-1322 has more than 2,000 binding sites in 1,058 genes with ΔG/ΔG m ratio of 85% and more. miR-1322 has 1,889 binding sites in CDSs, 215 binding sites in 5' UTRs, and 160 binding sites in 3' UTRs. From two to 28 binding sites have arranged localization with the start position through three nucleotides of each following binding site. The nucleotide sequences of these sites in CDSs encode oligopeptides with the same and/or different amino acid sequences. We found that 33% of the target genes encoded transcription factors. miR-1322 has arranged binding sites in the CDSs of orthologous MAMLD1, MAML2, and MAML3 genes. These sites encode a polyglutamine oligopeptide ranging from six to 47 amino acids in length. The properties of miR-1322 binding sites in orthologous and paralogous target genes are discussed.

No MeSH data available.


Conserved amino acid sequences containing polyglutamine in orthologous MAML3. Note: the number “9” indicates the number of glutamine residues in a site position of Fca protein (a).
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fig5: Conserved amino acid sequences containing polyglutamine in orthologous MAML3. Note: the number “9” indicates the number of glutamine residues in a site position of Fca protein (a).

Mentions: miR-1322 binding sites in orthologous MAML mRNAs are highly conserved. Orthologous MAML proteins have conserved amino acid sequences containing polyglutamine (Figures 3–5). Orthologous miRNAs are not identified in most animals except Pan troglodytes (chimpanzee) and Pongo pygmaeus (orangutan); however, some other miRNAs are identical or very similar to the corresponding human miRNAs. Therefore, human miRNAs were used for the subsequent identification of conserved binding sites. Oligonucleotides containing CAG repeats represent the miR-1322 binding site of the mRNA that encoded a long polyglutamine sequence in the corresponding protein. Oligonucleotides encoding polyglutamine are located in the conserved protein domain.


miR-1322 Binding Sites in Paralogous and Orthologous Genes.

Niyazova R, Berillo O, Atambayeva S, Pyrkova A, Alybayeva A, Ivashchenko A - Biomed Res Int (2015)

Conserved amino acid sequences containing polyglutamine in orthologous MAML3. Note: the number “9” indicates the number of glutamine residues in a site position of Fca protein (a).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465656&req=5

fig5: Conserved amino acid sequences containing polyglutamine in orthologous MAML3. Note: the number “9” indicates the number of glutamine residues in a site position of Fca protein (a).
Mentions: miR-1322 binding sites in orthologous MAML mRNAs are highly conserved. Orthologous MAML proteins have conserved amino acid sequences containing polyglutamine (Figures 3–5). Orthologous miRNAs are not identified in most animals except Pan troglodytes (chimpanzee) and Pongo pygmaeus (orangutan); however, some other miRNAs are identical or very similar to the corresponding human miRNAs. Therefore, human miRNAs were used for the subsequent identification of conserved binding sites. Oligonucleotides containing CAG repeats represent the miR-1322 binding site of the mRNA that encoded a long polyglutamine sequence in the corresponding protein. Oligonucleotides encoding polyglutamine are located in the conserved protein domain.

Bottom Line: From two to 28 binding sites have arranged localization with the start position through three nucleotides of each following binding site.The nucleotide sequences of these sites in CDSs encode oligopeptides with the same and/or different amino acid sequences.These sites encode a polyglutamine oligopeptide ranging from six to 47 amino acids in length.

View Article: PubMed Central - PubMed

Affiliation: National Nanotechnology Laboratory, Al-Farabi Kazakh National University, 71 Al-Farabi, Almaty 050038, Kazakhstan.

ABSTRACT
We searched for 2,563 microRNA (miRNA) binding sites in 17,494 mRNA sequences of human genes. miR-1322 has more than 2,000 binding sites in 1,058 genes with ΔG/ΔG m ratio of 85% and more. miR-1322 has 1,889 binding sites in CDSs, 215 binding sites in 5' UTRs, and 160 binding sites in 3' UTRs. From two to 28 binding sites have arranged localization with the start position through three nucleotides of each following binding site. The nucleotide sequences of these sites in CDSs encode oligopeptides with the same and/or different amino acid sequences. We found that 33% of the target genes encoded transcription factors. miR-1322 has arranged binding sites in the CDSs of orthologous MAMLD1, MAML2, and MAML3 genes. These sites encode a polyglutamine oligopeptide ranging from six to 47 amino acids in length. The properties of miR-1322 binding sites in orthologous and paralogous target genes are discussed.

No MeSH data available.