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Molecular Systematics of the Phoxinin Genus Pteronotropis (Otophysi: Cypriniformes).

Mayden RL, Allen JS - Biomed Res Int (2015)

Bottom Line: The habitats most frequently associated with these species include heavily vegetated backwater bayous to small sluggish or flowing tannin-stained streams.In no analysis was Pteronotropis, as currently recognized, recovered as monophyletic without the inclusion of the currently recognized Notropis harperi, herein referred to as Pteronotropis.Two major clades are supported: one inclusive of P. hubbsi, P. welaka, and P. harperi and the second inclusive of P. signipinnis, P. grandipinnis, P. hypselopterus plus P. merlini sister to P. euryzonus, and P. metallicus plus P. stonei.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Saint Louis University, 3507 Laclede Avenue, St. Louis, MO 63103, USA.

ABSTRACT
The genus Pteronotropis is widely distributed along the gulf slope of eastern North America from Louisiana to Florida and rivers in South Carolina along the Atlantic slope. Pteronotropis have very distinctive, flamboyant coloration. The habitats most frequently associated with these species include heavily vegetated backwater bayous to small sluggish or flowing tannin-stained streams. Although Pteronotropis is recognized as a valid genus, no phylogenetic analysis of all the species has corroborated its monophyly. In recent years, four additional species have been either described or elevated from synonymy: P. merlini, P. grandipinnis, P. stonei, and P. metallicus, with the wide-ranging P. hypselopterus complex. To examine relationships within this genus and test its monophyly, phylogenetic analyses were conducted using two nuclear genes, recombination activating gene 1, RAG1, and the first intron of S7 ribosomal protein gene in both maximum parsimony and Bayesian analyses. In no analysis was Pteronotropis, as currently recognized, recovered as monophyletic without the inclusion of the currently recognized Notropis harperi, herein referred to as Pteronotropis. Two major clades are supported: one inclusive of P. hubbsi, P. welaka, and P. harperi and the second inclusive of P. signipinnis, P. grandipinnis, P. hypselopterus plus P. merlini sister to P. euryzonus, and P. metallicus plus P. stonei.

No MeSH data available.


Inferred species relationships of species of Pteronotropis based on maximum parsimony and Bayesian analyses of combined Rag1 + S7 (a) and Rag1 + S7 (b), respectively. Nodal values indicate posterior probabilities.
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fig3: Inferred species relationships of species of Pteronotropis based on maximum parsimony and Bayesian analyses of combined Rag1 + S7 (a) and Rag1 + S7 (b), respectively. Nodal values indicate posterior probabilities.

Mentions: As the ILD test was nonsignificant for heterogeneity between RAG1 and S7, the gene sequences were analyzed both individually and as a concatenated data set. MP analysis of the aligned 1001 bp of S7 (aligned sequence lengths ranged from 839 to 919 bp) yielded 245 bp parsimony informative sites (12.9%). Analyses of these data resulted in 90 equally parsimonious trees (Figure 1; length = 697, CI = 0.803, and RI = 0.875). The more conservative RAG1 sequences included 1521 bp with 151 bp sites (9.9%) being parsimony informative. MP analyses of RAG1 resulted in 46,668 equally parsimonious trees (Figure 1; length = 371 steps, CI = 0.658, and RI = 0.866). Individual BA analyses for each gene resulted in some variations in sister-group relationships but all were consistent and supported the monophyly of Pteronotropis (Figure 2). Both MPA and BA of the combined S7 + RAG1 data recovered identical topologies (Figure 3).


Molecular Systematics of the Phoxinin Genus Pteronotropis (Otophysi: Cypriniformes).

Mayden RL, Allen JS - Biomed Res Int (2015)

Inferred species relationships of species of Pteronotropis based on maximum parsimony and Bayesian analyses of combined Rag1 + S7 (a) and Rag1 + S7 (b), respectively. Nodal values indicate posterior probabilities.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4465653&req=5

fig3: Inferred species relationships of species of Pteronotropis based on maximum parsimony and Bayesian analyses of combined Rag1 + S7 (a) and Rag1 + S7 (b), respectively. Nodal values indicate posterior probabilities.
Mentions: As the ILD test was nonsignificant for heterogeneity between RAG1 and S7, the gene sequences were analyzed both individually and as a concatenated data set. MP analysis of the aligned 1001 bp of S7 (aligned sequence lengths ranged from 839 to 919 bp) yielded 245 bp parsimony informative sites (12.9%). Analyses of these data resulted in 90 equally parsimonious trees (Figure 1; length = 697, CI = 0.803, and RI = 0.875). The more conservative RAG1 sequences included 1521 bp with 151 bp sites (9.9%) being parsimony informative. MP analyses of RAG1 resulted in 46,668 equally parsimonious trees (Figure 1; length = 371 steps, CI = 0.658, and RI = 0.866). Individual BA analyses for each gene resulted in some variations in sister-group relationships but all were consistent and supported the monophyly of Pteronotropis (Figure 2). Both MPA and BA of the combined S7 + RAG1 data recovered identical topologies (Figure 3).

Bottom Line: The habitats most frequently associated with these species include heavily vegetated backwater bayous to small sluggish or flowing tannin-stained streams.In no analysis was Pteronotropis, as currently recognized, recovered as monophyletic without the inclusion of the currently recognized Notropis harperi, herein referred to as Pteronotropis.Two major clades are supported: one inclusive of P. hubbsi, P. welaka, and P. harperi and the second inclusive of P. signipinnis, P. grandipinnis, P. hypselopterus plus P. merlini sister to P. euryzonus, and P. metallicus plus P. stonei.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Saint Louis University, 3507 Laclede Avenue, St. Louis, MO 63103, USA.

ABSTRACT
The genus Pteronotropis is widely distributed along the gulf slope of eastern North America from Louisiana to Florida and rivers in South Carolina along the Atlantic slope. Pteronotropis have very distinctive, flamboyant coloration. The habitats most frequently associated with these species include heavily vegetated backwater bayous to small sluggish or flowing tannin-stained streams. Although Pteronotropis is recognized as a valid genus, no phylogenetic analysis of all the species has corroborated its monophyly. In recent years, four additional species have been either described or elevated from synonymy: P. merlini, P. grandipinnis, P. stonei, and P. metallicus, with the wide-ranging P. hypselopterus complex. To examine relationships within this genus and test its monophyly, phylogenetic analyses were conducted using two nuclear genes, recombination activating gene 1, RAG1, and the first intron of S7 ribosomal protein gene in both maximum parsimony and Bayesian analyses. In no analysis was Pteronotropis, as currently recognized, recovered as monophyletic without the inclusion of the currently recognized Notropis harperi, herein referred to as Pteronotropis. Two major clades are supported: one inclusive of P. hubbsi, P. welaka, and P. harperi and the second inclusive of P. signipinnis, P. grandipinnis, P. hypselopterus plus P. merlini sister to P. euryzonus, and P. metallicus plus P. stonei.

No MeSH data available.