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Temporal and Spatial Variation in the Abundance of Total and Pathogenic Vibrio parahaemolyticus in Shellfish in China.

Han H, Li F, Yan W, Guo Y, Li N, Liu X, Zhu J, Xu J, Chen Y, Li X, Lv H, Zhang Y, Cai T, Chen Y - PLoS ONE (2015)

Bottom Line: The aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets.The log-transformed abundance of V. parahaemolyticus was significantly correlated with both water temperature and air temperature but not water salinity.These results provide baseline contamination data of V. parahaemolyticus in shellfish in China, which can be applied to local risk assessments to prioritize risk control to key sectors and evaluate the effectiveness of future control measures.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China; Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing, China.

ABSTRACT
We investigated the abundance of total and pathogenic Vibrio parahaemolyticus in shellfish sampled from four provinces in China during May 2013 and March 2014 using the most probable number-polymerase chain reaction (MPN-PCR) method. Total V. parahaemolyticus was detected in 67.7% of 496 samples. A total of 38.1% and 10.1% of samples exceeded 1,000 MPN g(-1) and 10,000 MPN g(-1), respectively. V. parahaemolyticus densities followed a seasonal and geographical trend, with Guangxi and Sichuan shellfish possessing total V. parahaemolyticus levels that were 100-fold higher than those of the Liaoning and Shandong regions. Moreover, the levels of V. parahaemolyticus were at least 10-fold higher in the summer and autumn than in the cooler seasons. Pathogenic V. parahaemolyticus levels were generally lower than total V. parahaemolyticus levels by several log units and tended to be high in samples contaminated with high total V. parahaemolyticus levels. The aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets. The catering markets showed the lowest levels of total V. parahaemolyticus, but 20.0% of samples exceeded 1,000 MPN g(-1). The levels of both total and pathogenic V. parahaemolyticus in oysters were higher than in clams. The log-transformed abundance of V. parahaemolyticus was significantly correlated with both water temperature and air temperature but not water salinity. These results provide baseline contamination data of V. parahaemolyticus in shellfish in China, which can be applied to local risk assessments to prioritize risk control to key sectors and evaluate the effectiveness of future control measures.

No MeSH data available.


Related in: MedlinePlus

Seasonal trends in V. parahaemolyticus densities in shellfish collected from the three sections.The X-axis represents the level of V. parahaemolyticus in the four seasons. Different symbols indicate samples from retail markets, aqua farms, and catering markets. Total and pathogenic V. parahaemolyticus densities were determined by MPN-PCR for tlh (A), tdh (B), trh (C), and ORF8 (D). The X- and Y-axes intersect at reciprocal values of -0.82, which equals the log10 of 0.15 MPN g-1.
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pone.0130302.g001: Seasonal trends in V. parahaemolyticus densities in shellfish collected from the three sections.The X-axis represents the level of V. parahaemolyticus in the four seasons. Different symbols indicate samples from retail markets, aqua farms, and catering markets. Total and pathogenic V. parahaemolyticus densities were determined by MPN-PCR for tlh (A), tdh (B), trh (C), and ORF8 (D). The X- and Y-axes intersect at reciprocal values of -0.82, which equals the log10 of 0.15 MPN g-1.

Mentions: Fig 1A illustrates the mean total V. parahaemolyticus levels in shellfish collected from three market categories in different seasons. Overall, V. parahaemolyticus contamination was found in all three market categories in all seasons (Fig 1A). Aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets (Table 1). Although catering markets had the lowest total V. parahaemolyticus densities, 20.0% of the samples exceeded 1,000 MPN g-1 (Table 1). Higher levels of V. parahaemolyticus in catering markets were found during the summer and winter, and the lowest values were found in autumn (Fig 1A). In the aqua farms and retail markets, the highest total V. parahaemolyticus levels were observed in the autumn than in the summer (Fig 1A).


Temporal and Spatial Variation in the Abundance of Total and Pathogenic Vibrio parahaemolyticus in Shellfish in China.

Han H, Li F, Yan W, Guo Y, Li N, Liu X, Zhu J, Xu J, Chen Y, Li X, Lv H, Zhang Y, Cai T, Chen Y - PLoS ONE (2015)

Seasonal trends in V. parahaemolyticus densities in shellfish collected from the three sections.The X-axis represents the level of V. parahaemolyticus in the four seasons. Different symbols indicate samples from retail markets, aqua farms, and catering markets. Total and pathogenic V. parahaemolyticus densities were determined by MPN-PCR for tlh (A), tdh (B), trh (C), and ORF8 (D). The X- and Y-axes intersect at reciprocal values of -0.82, which equals the log10 of 0.15 MPN g-1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465338&req=5

pone.0130302.g001: Seasonal trends in V. parahaemolyticus densities in shellfish collected from the three sections.The X-axis represents the level of V. parahaemolyticus in the four seasons. Different symbols indicate samples from retail markets, aqua farms, and catering markets. Total and pathogenic V. parahaemolyticus densities were determined by MPN-PCR for tlh (A), tdh (B), trh (C), and ORF8 (D). The X- and Y-axes intersect at reciprocal values of -0.82, which equals the log10 of 0.15 MPN g-1.
Mentions: Fig 1A illustrates the mean total V. parahaemolyticus levels in shellfish collected from three market categories in different seasons. Overall, V. parahaemolyticus contamination was found in all three market categories in all seasons (Fig 1A). Aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets (Table 1). Although catering markets had the lowest total V. parahaemolyticus densities, 20.0% of the samples exceeded 1,000 MPN g-1 (Table 1). Higher levels of V. parahaemolyticus in catering markets were found during the summer and winter, and the lowest values were found in autumn (Fig 1A). In the aqua farms and retail markets, the highest total V. parahaemolyticus levels were observed in the autumn than in the summer (Fig 1A).

Bottom Line: The aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets.The log-transformed abundance of V. parahaemolyticus was significantly correlated with both water temperature and air temperature but not water salinity.These results provide baseline contamination data of V. parahaemolyticus in shellfish in China, which can be applied to local risk assessments to prioritize risk control to key sectors and evaluate the effectiveness of future control measures.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China; Key Laboratory of Food Safety Risk Assessment of Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing, China.

ABSTRACT
We investigated the abundance of total and pathogenic Vibrio parahaemolyticus in shellfish sampled from four provinces in China during May 2013 and March 2014 using the most probable number-polymerase chain reaction (MPN-PCR) method. Total V. parahaemolyticus was detected in 67.7% of 496 samples. A total of 38.1% and 10.1% of samples exceeded 1,000 MPN g(-1) and 10,000 MPN g(-1), respectively. V. parahaemolyticus densities followed a seasonal and geographical trend, with Guangxi and Sichuan shellfish possessing total V. parahaemolyticus levels that were 100-fold higher than those of the Liaoning and Shandong regions. Moreover, the levels of V. parahaemolyticus were at least 10-fold higher in the summer and autumn than in the cooler seasons. Pathogenic V. parahaemolyticus levels were generally lower than total V. parahaemolyticus levels by several log units and tended to be high in samples contaminated with high total V. parahaemolyticus levels. The aqua farms had a lower prevalence but higher abundance of total V. parahaemolyticus compared to retail markets. The catering markets showed the lowest levels of total V. parahaemolyticus, but 20.0% of samples exceeded 1,000 MPN g(-1). The levels of both total and pathogenic V. parahaemolyticus in oysters were higher than in clams. The log-transformed abundance of V. parahaemolyticus was significantly correlated with both water temperature and air temperature but not water salinity. These results provide baseline contamination data of V. parahaemolyticus in shellfish in China, which can be applied to local risk assessments to prioritize risk control to key sectors and evaluate the effectiveness of future control measures.

No MeSH data available.


Related in: MedlinePlus