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A New Synthetic Amphiploid (AADDAA) between Gossypium hirsutum and G. arboreum Lays the Foundation for Transferring Resistances to Verticillium and Drought.

Chen Y, Wang Y, Zhao T, Yang J, Feng S, Nazeer W, Zhang T, Zhou B - PLoS ONE (2015)

Bottom Line: Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two parents for transferring favorable genes from G. arboreum into G. hirsutum.Preliminary assessments of resistance at seedling stage indicate that the synthetic amphiploid showed highly resistant to Verticillium and drought.The synthetic amphiploid between G. hirsutum × G. arboreum would lay the foundation for developing G. arboreum-introgressed lines with the uniform genetic background of G. hirsutum acc TM-1, which would greatly enhance and simplify the mining, isolation, characterization, cloning and use of G. arboreum-specific desirable genes in future cotton breeding programs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, People's Republic of China.

ABSTRACT
Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. However, it is quite difficult to transfer favorable traits into Upland cotton through conventional methods due to the cross-incompatibility of G. hirsutum (2n = 52, AADD) and G. arboreum. Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two parents for transferring favorable genes from G. arboreum into G. hirsutum. Our results indicate that MSB2K supplemented with 0.5 mg l(-1) kinetin and 250 mg(-1) casein hydrolysate is an efficient initial medium for rescuing early (3 d after pollination) hybrid embryos. Eight putative hybrids were successfully obtained, which were further verified and characterized by cytology, molecular markers and morphological analysis. The putative hybrids were subsequently treated with different concentrations of colchicine solution to double their chromosomes. The results demonstrate that four putative hybrid plants were successfully chromosome-doubled by treatment with 0.1% colchicine for 24 h and become amphiploid, which were confirmed by cytological observation, self-fertilization and backcrossing. Preliminary assessments of resistance at seedling stage indicate that the synthetic amphiploid showed highly resistant to Verticillium and drought. The synthetic amphiploid between G. hirsutum × G. arboreum would lay the foundation for developing G. arboreum-introgressed lines with the uniform genetic background of G. hirsutum acc TM-1, which would greatly enhance and simplify the mining, isolation, characterization, cloning and use of G. arboreum-specific desirable genes in future cotton breeding programs.

No MeSH data available.


Related in: MedlinePlus

Rate of disease index during 35 days infected by Verticillium dahliae isolate V991.The experiments were repeated in three replicates. The error bars were calculated based on three biological replicates using standard deviation.
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pone.0128981.g007: Rate of disease index during 35 days infected by Verticillium dahliae isolate V991.The experiments were repeated in three replicates. The error bars were calculated based on three biological replicates using standard deviation.

Mentions: Verticillium wilt (VW) is a destructive disease for cotton, which results into huge loss of lint yield but also deterioration of fiber quality worldwide. Here, we test the resistance against Verticillium dahlia of two parents and S1 by the strong defoliating isolate V991. G. hirsutum var. Junmian1 is highly sensitive to VW as control. Verticillium wilt disease index was investigated from the beginning of the 11th day of onset after inoculation to 35th days, the survey once every three days. The results indicate that disease incidence and severity, and its onset of VW were significant different (Figs 7 and 8). Junmian 1 and TM-1 were severe and earlier onset of VW than Shixiya 1, while S1 was between TM-1 and Shixiya 1. At around 35 days after inoculation, distinct disease symptoms were apparent on all plants inoculated with V991. The disease index (DI) was 100% for Junmian1 and 77.2% for TM-1, but Shixiya1 showed very low DI, only 11.1%. And S1 was between TM-1 and Shixiya 1, about 36.7% of DI. The relative resistance index demonstrated that Junmian 1 and TM-1 are susceptible (S) to VW, S1 is tolerant (R) and Shixiya1 is resistant (HR) (Table 9), implying that Shixiya 1 possesses the potential for improving Upland cotton resistance to VW.


A New Synthetic Amphiploid (AADDAA) between Gossypium hirsutum and G. arboreum Lays the Foundation for Transferring Resistances to Verticillium and Drought.

Chen Y, Wang Y, Zhao T, Yang J, Feng S, Nazeer W, Zhang T, Zhou B - PLoS ONE (2015)

Rate of disease index during 35 days infected by Verticillium dahliae isolate V991.The experiments were repeated in three replicates. The error bars were calculated based on three biological replicates using standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4465178&req=5

pone.0128981.g007: Rate of disease index during 35 days infected by Verticillium dahliae isolate V991.The experiments were repeated in three replicates. The error bars were calculated based on three biological replicates using standard deviation.
Mentions: Verticillium wilt (VW) is a destructive disease for cotton, which results into huge loss of lint yield but also deterioration of fiber quality worldwide. Here, we test the resistance against Verticillium dahlia of two parents and S1 by the strong defoliating isolate V991. G. hirsutum var. Junmian1 is highly sensitive to VW as control. Verticillium wilt disease index was investigated from the beginning of the 11th day of onset after inoculation to 35th days, the survey once every three days. The results indicate that disease incidence and severity, and its onset of VW were significant different (Figs 7 and 8). Junmian 1 and TM-1 were severe and earlier onset of VW than Shixiya 1, while S1 was between TM-1 and Shixiya 1. At around 35 days after inoculation, distinct disease symptoms were apparent on all plants inoculated with V991. The disease index (DI) was 100% for Junmian1 and 77.2% for TM-1, but Shixiya1 showed very low DI, only 11.1%. And S1 was between TM-1 and Shixiya 1, about 36.7% of DI. The relative resistance index demonstrated that Junmian 1 and TM-1 are susceptible (S) to VW, S1 is tolerant (R) and Shixiya1 is resistant (HR) (Table 9), implying that Shixiya 1 possesses the potential for improving Upland cotton resistance to VW.

Bottom Line: Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two parents for transferring favorable genes from G. arboreum into G. hirsutum.Preliminary assessments of resistance at seedling stage indicate that the synthetic amphiploid showed highly resistant to Verticillium and drought.The synthetic amphiploid between G. hirsutum × G. arboreum would lay the foundation for developing G. arboreum-introgressed lines with the uniform genetic background of G. hirsutum acc TM-1, which would greatly enhance and simplify the mining, isolation, characterization, cloning and use of G. arboreum-specific desirable genes in future cotton breeding programs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, People's Republic of China.

ABSTRACT
Gossypium arboreum, a cultivated cotton species (2n = 26, AA) native to Asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. However, it is quite difficult to transfer favorable traits into Upland cotton through conventional methods due to the cross-incompatibility of G. hirsutum (2n = 52, AADD) and G. arboreum. Here, we improved an embryo rescue technique to overcome the cross-incompatibility between these two parents for transferring favorable genes from G. arboreum into G. hirsutum. Our results indicate that MSB2K supplemented with 0.5 mg l(-1) kinetin and 250 mg(-1) casein hydrolysate is an efficient initial medium for rescuing early (3 d after pollination) hybrid embryos. Eight putative hybrids were successfully obtained, which were further verified and characterized by cytology, molecular markers and morphological analysis. The putative hybrids were subsequently treated with different concentrations of colchicine solution to double their chromosomes. The results demonstrate that four putative hybrid plants were successfully chromosome-doubled by treatment with 0.1% colchicine for 24 h and become amphiploid, which were confirmed by cytological observation, self-fertilization and backcrossing. Preliminary assessments of resistance at seedling stage indicate that the synthetic amphiploid showed highly resistant to Verticillium and drought. The synthetic amphiploid between G. hirsutum × G. arboreum would lay the foundation for developing G. arboreum-introgressed lines with the uniform genetic background of G. hirsutum acc TM-1, which would greatly enhance and simplify the mining, isolation, characterization, cloning and use of G. arboreum-specific desirable genes in future cotton breeding programs.

No MeSH data available.


Related in: MedlinePlus