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OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes.

Xu K, Chen S, Li T, Ma X, Liang X, Ding X, Liu H, Luo L - BMC Plant Biol. (2015)

Bottom Line: Drought is a major abiotic stress factors that reduces agricultural productivity.OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants.The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Agrobiological Gene Center, Shanghai, 201106, China. kxu@sagc.org.cn.

ABSTRACT

Background: Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice drought resistance have not been characterized.

Results: In this study, a novel GRAS transcription factor gene named OsGRAS23, which is located in a drought-resistant QTL interval on chromosome 4 of rice, was isolated. The expression of OsGRAS23 was induced by drought, NaCl, and jasmonic acid treatments. The OsGRAS23-GFP fused protein was localized in the nucleus of tobacco epidermal cells. A trans-activation assay in yeast cells demonstrated that the OsGRAS23 protein possessed a strong transcriptional activation activity. OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants. Furthermore, microarray analysis showed that several anti-oxidation related genes were up-regulated in the OsGRAS23-overexpressing rice plants. The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

Conclusions: Our results demonstrate that OsGRAS23 encodes a stress-responsive GRAS transcription factor and positively modulates rice drought tolerance via the induction of a number of stress-responsive genes.

No MeSH data available.


Related in: MedlinePlus

Drought resistance assay of OsGRAS23-overexpressing transgenic rice. a Three-week-old transgenic rice plants and WT plants grown in the 96-well plates and cultivated with culture solution, and exposed to dehydration stress for one day before being transferred to the culture solution. b Survival rate of WT and transgenic lines after dehydration stress. The data represent the mean ± SD (n = 3),*P ≤ 0.05. c, d Seed setting rate and yield of transgenic rice plants under drought stress at the reproductive stage. The data represent the mean ± SD (n = 8),*P ≤ 0.05, ** P ≤ 0.01, t-test
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Fig4: Drought resistance assay of OsGRAS23-overexpressing transgenic rice. a Three-week-old transgenic rice plants and WT plants grown in the 96-well plates and cultivated with culture solution, and exposed to dehydration stress for one day before being transferred to the culture solution. b Survival rate of WT and transgenic lines after dehydration stress. The data represent the mean ± SD (n = 3),*P ≤ 0.05. c, d Seed setting rate and yield of transgenic rice plants under drought stress at the reproductive stage. The data represent the mean ± SD (n = 8),*P ≤ 0.05, ** P ≤ 0.01, t-test

Mentions: Under the dehydration stress condition, OsGRAS23-overexpressing rice plants showed a lower degree of wilting and better recovery compared with WT plants (Fig. 4A), and 37-45 % of OsGRAS23-overexpressing plants recovered, which was significantly more than that of the WT plants (Fig. 4B). When grown in a paddy field under drought stress during the later tillering stage, the seed setting rate and yield of OsGRAS23-overexpressing plants (OE1 and OE20 lines) were significantly higher than those of the WT plants (Fig. 4C and D). These results indicated that overexpression of OsGRAS23 could improve the drought resistance of the transgenic rice plants.Fig. 4


OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes.

Xu K, Chen S, Li T, Ma X, Liang X, Ding X, Liu H, Luo L - BMC Plant Biol. (2015)

Drought resistance assay of OsGRAS23-overexpressing transgenic rice. a Three-week-old transgenic rice plants and WT plants grown in the 96-well plates and cultivated with culture solution, and exposed to dehydration stress for one day before being transferred to the culture solution. b Survival rate of WT and transgenic lines after dehydration stress. The data represent the mean ± SD (n = 3),*P ≤ 0.05. c, d Seed setting rate and yield of transgenic rice plants under drought stress at the reproductive stage. The data represent the mean ± SD (n = 8),*P ≤ 0.05, ** P ≤ 0.01, t-test
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4465154&req=5

Fig4: Drought resistance assay of OsGRAS23-overexpressing transgenic rice. a Three-week-old transgenic rice plants and WT plants grown in the 96-well plates and cultivated with culture solution, and exposed to dehydration stress for one day before being transferred to the culture solution. b Survival rate of WT and transgenic lines after dehydration stress. The data represent the mean ± SD (n = 3),*P ≤ 0.05. c, d Seed setting rate and yield of transgenic rice plants under drought stress at the reproductive stage. The data represent the mean ± SD (n = 8),*P ≤ 0.05, ** P ≤ 0.01, t-test
Mentions: Under the dehydration stress condition, OsGRAS23-overexpressing rice plants showed a lower degree of wilting and better recovery compared with WT plants (Fig. 4A), and 37-45 % of OsGRAS23-overexpressing plants recovered, which was significantly more than that of the WT plants (Fig. 4B). When grown in a paddy field under drought stress during the later tillering stage, the seed setting rate and yield of OsGRAS23-overexpressing plants (OE1 and OE20 lines) were significantly higher than those of the WT plants (Fig. 4C and D). These results indicated that overexpression of OsGRAS23 could improve the drought resistance of the transgenic rice plants.Fig. 4

Bottom Line: Drought is a major abiotic stress factors that reduces agricultural productivity.OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants.The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Agrobiological Gene Center, Shanghai, 201106, China. kxu@sagc.org.cn.

ABSTRACT

Background: Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice drought resistance have not been characterized.

Results: In this study, a novel GRAS transcription factor gene named OsGRAS23, which is located in a drought-resistant QTL interval on chromosome 4 of rice, was isolated. The expression of OsGRAS23 was induced by drought, NaCl, and jasmonic acid treatments. The OsGRAS23-GFP fused protein was localized in the nucleus of tobacco epidermal cells. A trans-activation assay in yeast cells demonstrated that the OsGRAS23 protein possessed a strong transcriptional activation activity. OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants. Furthermore, microarray analysis showed that several anti-oxidation related genes were up-regulated in the OsGRAS23-overexpressing rice plants. The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

Conclusions: Our results demonstrate that OsGRAS23 encodes a stress-responsive GRAS transcription factor and positively modulates rice drought tolerance via the induction of a number of stress-responsive genes.

No MeSH data available.


Related in: MedlinePlus