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OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes.

Xu K, Chen S, Li T, Ma X, Liang X, Ding X, Liu H, Luo L - BMC Plant Biol. (2015)

Bottom Line: Drought is a major abiotic stress factors that reduces agricultural productivity.OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants.The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Agrobiological Gene Center, Shanghai, 201106, China. kxu@sagc.org.cn.

ABSTRACT

Background: Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice drought resistance have not been characterized.

Results: In this study, a novel GRAS transcription factor gene named OsGRAS23, which is located in a drought-resistant QTL interval on chromosome 4 of rice, was isolated. The expression of OsGRAS23 was induced by drought, NaCl, and jasmonic acid treatments. The OsGRAS23-GFP fused protein was localized in the nucleus of tobacco epidermal cells. A trans-activation assay in yeast cells demonstrated that the OsGRAS23 protein possessed a strong transcriptional activation activity. OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants. Furthermore, microarray analysis showed that several anti-oxidation related genes were up-regulated in the OsGRAS23-overexpressing rice plants. The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

Conclusions: Our results demonstrate that OsGRAS23 encodes a stress-responsive GRAS transcription factor and positively modulates rice drought tolerance via the induction of a number of stress-responsive genes.

No MeSH data available.


Related in: MedlinePlus

Expression patterns of OsGRAS23. a Relative expression level of OsGRAS23 under hormone treatment including ABA (100 μM), GA (100 μM), and JA (100 μM). b Relative expression level of OsGRAS23 under stresses include PEG6000 (20 %), dehydration, and NaCl(100 mM). c Relative expression of OsGRAS23 in different tissues (root, stem, leaves, sheath and panicles) of rice plants under normal condition. All the expression levels of OsGARS-23 were examined by quantitative real-time PCR. The data represent the mean ± SE (n = 3). d OsGRAS23 promoter: GUS expression pattern in transgenic rice plants. GUS staining in the leaves, sheath (2), spike (3), stem (4), root (5), and root tip (6)
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Fig2: Expression patterns of OsGRAS23. a Relative expression level of OsGRAS23 under hormone treatment including ABA (100 μM), GA (100 μM), and JA (100 μM). b Relative expression level of OsGRAS23 under stresses include PEG6000 (20 %), dehydration, and NaCl(100 mM). c Relative expression of OsGRAS23 in different tissues (root, stem, leaves, sheath and panicles) of rice plants under normal condition. All the expression levels of OsGARS-23 were examined by quantitative real-time PCR. The data represent the mean ± SE (n = 3). d OsGRAS23 promoter: GUS expression pattern in transgenic rice plants. GUS staining in the leaves, sheath (2), spike (3), stem (4), root (5), and root tip (6)

Mentions: To investigate and predict the function of OsGRAS23, its expression patterns under various stresses and phytohormone treatments were investigated by real-time quantitative RT-PCR. As shown in Fig. 2, the expression of OsGRAS23 was significantly induced by polyethylene glycol (PEG), dehydration, salt, GA, and JA treatment in the rice leaves.Fig. 2


OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes.

Xu K, Chen S, Li T, Ma X, Liang X, Ding X, Liu H, Luo L - BMC Plant Biol. (2015)

Expression patterns of OsGRAS23. a Relative expression level of OsGRAS23 under hormone treatment including ABA (100 μM), GA (100 μM), and JA (100 μM). b Relative expression level of OsGRAS23 under stresses include PEG6000 (20 %), dehydration, and NaCl(100 mM). c Relative expression of OsGRAS23 in different tissues (root, stem, leaves, sheath and panicles) of rice plants under normal condition. All the expression levels of OsGARS-23 were examined by quantitative real-time PCR. The data represent the mean ± SE (n = 3). d OsGRAS23 promoter: GUS expression pattern in transgenic rice plants. GUS staining in the leaves, sheath (2), spike (3), stem (4), root (5), and root tip (6)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4465154&req=5

Fig2: Expression patterns of OsGRAS23. a Relative expression level of OsGRAS23 under hormone treatment including ABA (100 μM), GA (100 μM), and JA (100 μM). b Relative expression level of OsGRAS23 under stresses include PEG6000 (20 %), dehydration, and NaCl(100 mM). c Relative expression of OsGRAS23 in different tissues (root, stem, leaves, sheath and panicles) of rice plants under normal condition. All the expression levels of OsGARS-23 were examined by quantitative real-time PCR. The data represent the mean ± SE (n = 3). d OsGRAS23 promoter: GUS expression pattern in transgenic rice plants. GUS staining in the leaves, sheath (2), spike (3), stem (4), root (5), and root tip (6)
Mentions: To investigate and predict the function of OsGRAS23, its expression patterns under various stresses and phytohormone treatments were investigated by real-time quantitative RT-PCR. As shown in Fig. 2, the expression of OsGRAS23 was significantly induced by polyethylene glycol (PEG), dehydration, salt, GA, and JA treatment in the rice leaves.Fig. 2

Bottom Line: Drought is a major abiotic stress factors that reduces agricultural productivity.OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants.The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Agrobiological Gene Center, Shanghai, 201106, China. kxu@sagc.org.cn.

ABSTRACT

Background: Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice drought resistance have not been characterized.

Results: In this study, a novel GRAS transcription factor gene named OsGRAS23, which is located in a drought-resistant QTL interval on chromosome 4 of rice, was isolated. The expression of OsGRAS23 was induced by drought, NaCl, and jasmonic acid treatments. The OsGRAS23-GFP fused protein was localized in the nucleus of tobacco epidermal cells. A trans-activation assay in yeast cells demonstrated that the OsGRAS23 protein possessed a strong transcriptional activation activity. OsGRAS23-overexpressing rice plants showed improved drought resistance and oxidative stress tolerance as well as less H2O2 accumulation compared with the wild-type plants. Furthermore, microarray analysis showed that several anti-oxidation related genes were up-regulated in the OsGRAS23-overexpressing rice plants. The yeast one hybrid test indicated that OsGRAS23 could bind to the promoters of its potential target genes.

Conclusions: Our results demonstrate that OsGRAS23 encodes a stress-responsive GRAS transcription factor and positively modulates rice drought tolerance via the induction of a number of stress-responsive genes.

No MeSH data available.


Related in: MedlinePlus