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Tumor-related gene expression levels in pulmonary pleomorphic carcinoma.

Oyaizu T, Matsumura Y, Kobayashi S, Sado T, Ishihama H, Chida M - J Cardiothorac Surg (2015)

Bottom Line: Pulmonary pleomorphic carcinoma (PPC) is a rare type of non-small-cell lung cancer that belongs to the family of sarcomatoid carcinomas and is associated with poor prognosis.The gene expression levels of thymidylate synthase were significantly higher in PPC and SCC as compared to the AC specimens (p < 0.001).Furthermore, the expression level of excision repair cross-complementation group 1 mRNA in PPC specimens was similar to that reported in NSCLC, while the level of vascular endothelial growth factor (VEGF) expression was higher as compared to that reported for colorectal cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Thoracic Surgery, Dokkyo Medical University, Mibu, 321-0293, Japan. t-ouaizu@dokkyomed.ac.jp.

ABSTRACT

Background: Pulmonary pleomorphic carcinoma (PPC) is a rare type of non-small-cell lung cancer that belongs to the family of sarcomatoid carcinomas and is associated with poor prognosis. We investigated the expressions of tumor-related genes in resected PPC specimens.

Methods: Specimens resected from patients with PPC from July 2006 through April 2012 were investigated. Tumor segments were collected from the specimens by micro-dissection to extract mRNA, then RT-PCR was performed according to Dannenberg's tumor profile method for semi-quantitation of tumor-related gene mRNA. To compare with other types of lung cancer, data from stage-matched adenocarcinoma (AC) and squamous cell carcinoma (SCC) cases in our database were also examined.

Results: The gene expression levels of thymidylate synthase were significantly higher in PPC and SCC as compared to the AC specimens (p < 0.001). The levels of dihydropyrimidine dehydrogenase and thymidine phosphorylase mRNA in PPC showed a similar tendency to those in SCC, in contrast to AC. Furthermore, the expression level of excision repair cross-complementation group 1 mRNA in PPC specimens was similar to that reported in NSCLC, while the level of vascular endothelial growth factor (VEGF) expression was higher as compared to that reported for colorectal cancer.

Conclusions: Although gene expression of tumor cannot be directly correlated to its sensitivity for anti-cancer drugs, it is likely that PPC tumors are not sensitive to anti-metabolic drugs. Anti-VEGF therapy may be a candidate for PPC, while cisplatin also remains a viable option.

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Comparison of relative gene expression of thymidylate synthase in cancer specimens. PPC, pulmonary pleomorphic carcinoma; AC, adenocarcinoma; SCC, squamous cell carcinoma
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Fig1: Comparison of relative gene expression of thymidylate synthase in cancer specimens. PPC, pulmonary pleomorphic carcinoma; AC, adenocarcinoma; SCC, squamous cell carcinoma

Mentions: Gene expression levels of the nucleotide-metabolism-related enzymes TS, DPD, and TP in PPC were compared to those in AC and SCC specimens. The relative gene expression value of TS was 5.65 ± 3.46 in PPC, 1.59 ± 0.86 in AC, and 4.68 ± 3.71 in SCC (Fig. 1). That value was significantly higher in PPC and SCC specimens as compared to AC (p < 0.001). In contrast, the relative gene expression value for DPD was 2.49 ± 1.70, 2.20 ± 1.18, and 1.59 ± 1.34, respectively (Fig. 2), which was greater in PPC and AC than in SCC, with borderline significance (p = 0.0649). That of TP in the specimens was 14.81 ± 14.99, 9.17 ± 6.51, and 14.61 ± 11.06, respectively (Fig. 3), with no significant difference between AC and SCC, while that of AC was significantly lower as compared to SCC (p < 0.05).Fig. 1


Tumor-related gene expression levels in pulmonary pleomorphic carcinoma.

Oyaizu T, Matsumura Y, Kobayashi S, Sado T, Ishihama H, Chida M - J Cardiothorac Surg (2015)

Comparison of relative gene expression of thymidylate synthase in cancer specimens. PPC, pulmonary pleomorphic carcinoma; AC, adenocarcinoma; SCC, squamous cell carcinoma
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4465152&req=5

Fig1: Comparison of relative gene expression of thymidylate synthase in cancer specimens. PPC, pulmonary pleomorphic carcinoma; AC, adenocarcinoma; SCC, squamous cell carcinoma
Mentions: Gene expression levels of the nucleotide-metabolism-related enzymes TS, DPD, and TP in PPC were compared to those in AC and SCC specimens. The relative gene expression value of TS was 5.65 ± 3.46 in PPC, 1.59 ± 0.86 in AC, and 4.68 ± 3.71 in SCC (Fig. 1). That value was significantly higher in PPC and SCC specimens as compared to AC (p < 0.001). In contrast, the relative gene expression value for DPD was 2.49 ± 1.70, 2.20 ± 1.18, and 1.59 ± 1.34, respectively (Fig. 2), which was greater in PPC and AC than in SCC, with borderline significance (p = 0.0649). That of TP in the specimens was 14.81 ± 14.99, 9.17 ± 6.51, and 14.61 ± 11.06, respectively (Fig. 3), with no significant difference between AC and SCC, while that of AC was significantly lower as compared to SCC (p < 0.05).Fig. 1

Bottom Line: Pulmonary pleomorphic carcinoma (PPC) is a rare type of non-small-cell lung cancer that belongs to the family of sarcomatoid carcinomas and is associated with poor prognosis.The gene expression levels of thymidylate synthase were significantly higher in PPC and SCC as compared to the AC specimens (p < 0.001).Furthermore, the expression level of excision repair cross-complementation group 1 mRNA in PPC specimens was similar to that reported in NSCLC, while the level of vascular endothelial growth factor (VEGF) expression was higher as compared to that reported for colorectal cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of General Thoracic Surgery, Dokkyo Medical University, Mibu, 321-0293, Japan. t-ouaizu@dokkyomed.ac.jp.

ABSTRACT

Background: Pulmonary pleomorphic carcinoma (PPC) is a rare type of non-small-cell lung cancer that belongs to the family of sarcomatoid carcinomas and is associated with poor prognosis. We investigated the expressions of tumor-related genes in resected PPC specimens.

Methods: Specimens resected from patients with PPC from July 2006 through April 2012 were investigated. Tumor segments were collected from the specimens by micro-dissection to extract mRNA, then RT-PCR was performed according to Dannenberg's tumor profile method for semi-quantitation of tumor-related gene mRNA. To compare with other types of lung cancer, data from stage-matched adenocarcinoma (AC) and squamous cell carcinoma (SCC) cases in our database were also examined.

Results: The gene expression levels of thymidylate synthase were significantly higher in PPC and SCC as compared to the AC specimens (p < 0.001). The levels of dihydropyrimidine dehydrogenase and thymidine phosphorylase mRNA in PPC showed a similar tendency to those in SCC, in contrast to AC. Furthermore, the expression level of excision repair cross-complementation group 1 mRNA in PPC specimens was similar to that reported in NSCLC, while the level of vascular endothelial growth factor (VEGF) expression was higher as compared to that reported for colorectal cancer.

Conclusions: Although gene expression of tumor cannot be directly correlated to its sensitivity for anti-cancer drugs, it is likely that PPC tumors are not sensitive to anti-metabolic drugs. Anti-VEGF therapy may be a candidate for PPC, while cisplatin also remains a viable option.

Show MeSH
Related in: MedlinePlus