Limits...
Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh.

Deng M, Gu Y, Liu Z, Qi Y, Ma GE, Kang N - Stem Cells Int (2015)

Bottom Line: Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34- when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells.Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs.This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype.

View Article: PubMed Central - PubMed

Affiliation: Liposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China.

ABSTRACT
Adipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D plastic. Cell adhesion, viability, and distribution of hADSCs on PGA/PLA mesh were observed by CM-Dil labeling, live/dead staining, and SEM examination while endothelial differentiation was evaluated by flow cytometry, Ac-LDL/UEA-1 uptake assay, immunofluorescence stainings, and gene expression analysis of endothelial related markers. Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34- when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells. Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs. This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype. We believed this study would be the initial step towards the generation of prevascularized tissue engineered constructs.

No MeSH data available.


Related in: MedlinePlus

Characterization of PGA/PLA mesh and hADSCs-PGA/PLA complex. Cylinder-shaped PGA/PLA mesh (a). SEM examination of PGA/PLA polymer (scale bars: 200 μm, (b)). Mechanical property of PGA fiber alone and PGA/PLA complex (c). CM-Dil labeled (stained red) hADSCs-PGA/PLA complex 3 days after seeding (scale bars: 200 μm, (d)). SEM examination of hADSCs-PGA/PLA complex with an initial cell seeding density of 10 × 106/mL cultured 14 days after seeding (scale bars: 500 μm, (e)). Live (stained green) and dead staining (stained red) of hADSCs-PGA/PLA complex 21 days after seeding visualized by laser confocal microscopy (scale bars: 100 μm, (f)).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4464689&req=5

fig2: Characterization of PGA/PLA mesh and hADSCs-PGA/PLA complex. Cylinder-shaped PGA/PLA mesh (a). SEM examination of PGA/PLA polymer (scale bars: 200 μm, (b)). Mechanical property of PGA fiber alone and PGA/PLA complex (c). CM-Dil labeled (stained red) hADSCs-PGA/PLA complex 3 days after seeding (scale bars: 200 μm, (d)). SEM examination of hADSCs-PGA/PLA complex with an initial cell seeding density of 10 × 106/mL cultured 14 days after seeding (scale bars: 500 μm, (e)). Live (stained green) and dead staining (stained red) of hADSCs-PGA/PLA complex 21 days after seeding visualized by laser confocal microscopy (scale bars: 100 μm, (f)).

Mentions: PGA/PLA scaffold was fabricated into a cylinder shape of 5 mm in diameter and 0.8 mm in thickness (Figure 2(a)). SEM showed that the PGA fibers were 30.62 ± 2.8 μm in diameter, and the PLA adhering between polymer fibers assembled the PGA into a mesh with various sizes of pores (~200 μm, Figure 2(b)). PGA coated with PLA exhibited a higher mechanical property in Young's modulus (3.17 ± 0.54 MPa) compared to PGA fibers alone (0.66 ± 0.31 MPa) (Figure 2(c)).


Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh.

Deng M, Gu Y, Liu Z, Qi Y, Ma GE, Kang N - Stem Cells Int (2015)

Characterization of PGA/PLA mesh and hADSCs-PGA/PLA complex. Cylinder-shaped PGA/PLA mesh (a). SEM examination of PGA/PLA polymer (scale bars: 200 μm, (b)). Mechanical property of PGA fiber alone and PGA/PLA complex (c). CM-Dil labeled (stained red) hADSCs-PGA/PLA complex 3 days after seeding (scale bars: 200 μm, (d)). SEM examination of hADSCs-PGA/PLA complex with an initial cell seeding density of 10 × 106/mL cultured 14 days after seeding (scale bars: 500 μm, (e)). Live (stained green) and dead staining (stained red) of hADSCs-PGA/PLA complex 21 days after seeding visualized by laser confocal microscopy (scale bars: 100 μm, (f)).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4464689&req=5

fig2: Characterization of PGA/PLA mesh and hADSCs-PGA/PLA complex. Cylinder-shaped PGA/PLA mesh (a). SEM examination of PGA/PLA polymer (scale bars: 200 μm, (b)). Mechanical property of PGA fiber alone and PGA/PLA complex (c). CM-Dil labeled (stained red) hADSCs-PGA/PLA complex 3 days after seeding (scale bars: 200 μm, (d)). SEM examination of hADSCs-PGA/PLA complex with an initial cell seeding density of 10 × 106/mL cultured 14 days after seeding (scale bars: 500 μm, (e)). Live (stained green) and dead staining (stained red) of hADSCs-PGA/PLA complex 21 days after seeding visualized by laser confocal microscopy (scale bars: 100 μm, (f)).
Mentions: PGA/PLA scaffold was fabricated into a cylinder shape of 5 mm in diameter and 0.8 mm in thickness (Figure 2(a)). SEM showed that the PGA fibers were 30.62 ± 2.8 μm in diameter, and the PLA adhering between polymer fibers assembled the PGA into a mesh with various sizes of pores (~200 μm, Figure 2(b)). PGA coated with PLA exhibited a higher mechanical property in Young's modulus (3.17 ± 0.54 MPa) compared to PGA fibers alone (0.66 ± 0.31 MPa) (Figure 2(c)).

Bottom Line: Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34- when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells.Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs.This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype.

View Article: PubMed Central - PubMed

Affiliation: Liposuction Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China.

ABSTRACT
Adipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D plastic. Cell adhesion, viability, and distribution of hADSCs on PGA/PLA mesh were observed by CM-Dil labeling, live/dead staining, and SEM examination while endothelial differentiation was evaluated by flow cytometry, Ac-LDL/UEA-1 uptake assay, immunofluorescence stainings, and gene expression analysis of endothelial related markers. Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34- when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells. Moreover, compared to 2D groups, the expression for α-SMA was markedly suppressed in 3D cultured hADSCs. This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype. We believed this study would be the initial step towards the generation of prevascularized tissue engineered constructs.

No MeSH data available.


Related in: MedlinePlus