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Protective Effect of Neuropeptide Substance P on Bone Marrow Mesenchymal Stem Cells against Apoptosis Induced by Serum Deprivation.

Fu S, Jin D, Liu S, Wang L, Wang Z, Mei G, Zou ZL, Wu JQ, Xu ZY - Stem Cells Int (2015)

Bottom Line: Substance P (SP) contributes to bone formation by stimulating the proliferation and differentiation of bone marrow stromal cells (BMSCs); however, the possible involved effect of SP on apoptosis induced by serum deprivation (SD) in BMSCs is unclear.To explore the potential protective effect of SP and its mechanism, we investigated the relationships among SP, apoptosis induced by SD, and Wnt signaling in BMSCs.Our results revealed that mediated by the NK-1 receptor, SP exerts an inhibitory effect on serum deprivation induced apoptosis in BMSCs that is related to the activation of canonical Wnt signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.

ABSTRACT
Substance P (SP) contributes to bone formation by stimulating the proliferation and differentiation of bone marrow stromal cells (BMSCs); however, the possible involved effect of SP on apoptosis induced by serum deprivation (SD) in BMSCs is unclear. To explore the potential protective effect of SP and its mechanism, we investigated the relationships among SP, apoptosis induced by SD, and Wnt signaling in BMSCs. SP exhibited a protective effect, as indicated by a reduction in the apoptotic rate, nuclear condensation, caspase-3 and caspase-9 activation, and the ratio of Bax/Bcl-2 that was observed after 24 h of SD. This protective effect was blocked by the inhibition of Wnt signaling or antagonism of the NK-1 receptor. Moreover, SP promoted the mRNA and protein expression of Wnt signaling molecules such as β-catenin, p-GSK-3β, c-myc, and cyclin D1 in addition to the nuclear translocation of β-catenin, indicating that active Wnt signaling is involved in SP inhibition of apoptosis. Our results revealed that mediated by the NK-1 receptor, SP exerts an inhibitory effect on serum deprivation induced apoptosis in BMSCs that is related to the activation of canonical Wnt signaling.

No MeSH data available.


Related in: MedlinePlus

Preparation of BMSCs. The cultured cells were identified with FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%).
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fig1: Preparation of BMSCs. The cultured cells were identified with FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%).

Mentions: The cultured BMSCs were examined under an optical microscope with a spindle shaped morphology. Following passage of the third generation culture, cells grew faster than the original generation. The results of the flow cytometry showed that the cultured cells were FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%), which indicated that the cells were BMSCs (Figure 1).


Protective Effect of Neuropeptide Substance P on Bone Marrow Mesenchymal Stem Cells against Apoptosis Induced by Serum Deprivation.

Fu S, Jin D, Liu S, Wang L, Wang Z, Mei G, Zou ZL, Wu JQ, Xu ZY - Stem Cells Int (2015)

Preparation of BMSCs. The cultured cells were identified with FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4464676&req=5

fig1: Preparation of BMSCs. The cultured cells were identified with FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%).
Mentions: The cultured BMSCs were examined under an optical microscope with a spindle shaped morphology. Following passage of the third generation culture, cells grew faster than the original generation. The results of the flow cytometry showed that the cultured cells were FL1-Height (0.2% ± 1.7%), CD90 (99.7% ± 3.1%), IgG1 (0.96% ± 1.3%), CD34 (1.06% ± 1.3%), CD44 (99.8% ± 4.1%), CD45 (1.7% ± 2.1%), IgG2a (0.35% ± 2.9%), CD11b/C (0.6% ± 1.7%), HAMSTER (0.09% ± 0.7%), and CD29 (99.88% ± 3.1%), which indicated that the cells were BMSCs (Figure 1).

Bottom Line: Substance P (SP) contributes to bone formation by stimulating the proliferation and differentiation of bone marrow stromal cells (BMSCs); however, the possible involved effect of SP on apoptosis induced by serum deprivation (SD) in BMSCs is unclear.To explore the potential protective effect of SP and its mechanism, we investigated the relationships among SP, apoptosis induced by SD, and Wnt signaling in BMSCs.Our results revealed that mediated by the NK-1 receptor, SP exerts an inhibitory effect on serum deprivation induced apoptosis in BMSCs that is related to the activation of canonical Wnt signaling.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University 1838, North Guangzhou Avenue, Guangzhou, Guangdong 510515, China.

ABSTRACT
Substance P (SP) contributes to bone formation by stimulating the proliferation and differentiation of bone marrow stromal cells (BMSCs); however, the possible involved effect of SP on apoptosis induced by serum deprivation (SD) in BMSCs is unclear. To explore the potential protective effect of SP and its mechanism, we investigated the relationships among SP, apoptosis induced by SD, and Wnt signaling in BMSCs. SP exhibited a protective effect, as indicated by a reduction in the apoptotic rate, nuclear condensation, caspase-3 and caspase-9 activation, and the ratio of Bax/Bcl-2 that was observed after 24 h of SD. This protective effect was blocked by the inhibition of Wnt signaling or antagonism of the NK-1 receptor. Moreover, SP promoted the mRNA and protein expression of Wnt signaling molecules such as β-catenin, p-GSK-3β, c-myc, and cyclin D1 in addition to the nuclear translocation of β-catenin, indicating that active Wnt signaling is involved in SP inhibition of apoptosis. Our results revealed that mediated by the NK-1 receptor, SP exerts an inhibitory effect on serum deprivation induced apoptosis in BMSCs that is related to the activation of canonical Wnt signaling.

No MeSH data available.


Related in: MedlinePlus