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Cell proliferation and apoptosis in urinary bladder urothelium of rats following ovariectomy and chronic estrogen replacement therapy.

Lucan L, Stamatian F, Lucan V, Tabaran AF - Clujul Med (2013)

Bottom Line: Proliferative and apoptotic activity was monitored by quantifying the urothelium imunoexpression for PCNA antigen as a marker of S phase of the cell cycle and Cleaved Caspase 3 for monitoring apoptotic activity.Estrogen therapy managed to improve the urothelium activity by reducing the Apoptotic Index and by increasing urothelial proliferative activity.The results show the important role of estrogens in maintaining urothelial activities, highlighting their potential use in the treatment of urothelium atrophic and degenerative processes associated with menopause.

View Article: PubMed Central - PubMed

Affiliation: 1 Gynecology Clinic, Iuliu HaĊ£ieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.

ABSTRACT

Aims: In this study we followed the effect of menopause and estrogenic replacement therapy on the proliferative and apoptotic activity of the bladder urothelial cells.

Methods: The experimental model of menopause was reproduced using a standard protocol of bilateral ovariectomy in rats, estrogen replacement therapy being achieved by systemic administration of hexestrol diacetate for six weeks. Proliferative and apoptotic activity was monitored by quantifying the urothelium imunoexpression for PCNA antigen as a marker of S phase of the cell cycle and Cleaved Caspase 3 for monitoring apoptotic activity.

Results: Following ovariectomy, the main changes were urothelial atrophy associated with intensification of the apoptotic activity at these level. Estrogen therapy managed to improve the urothelium activity by reducing the Apoptotic Index and by increasing urothelial proliferative activity.

Conclusions: The results show the important role of estrogens in maintaining urothelial activities, highlighting their potential use in the treatment of urothelium atrophic and degenerative processes associated with menopause.

No MeSH data available.


Related in: MedlinePlus

Histopathological findings and immune expression of PCNA and Caspase 3 in studied groups. On the first line of images arrows are indicating the apoptotic cells and the atrophy of the urothelium. In the second and third lines arrows indicates the immunoexpresion for PCNA and Caspase 3.
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f1-cm-86-27: Histopathological findings and immune expression of PCNA and Caspase 3 in studied groups. On the first line of images arrows are indicating the apoptotic cells and the atrophy of the urothelium. In the second and third lines arrows indicates the immunoexpresion for PCNA and Caspase 3.

Mentions: The histopathological aspect observed in the urothelium of the BOV group was the marked atrophy, aspect suggested by the decreased thickness of urothelium, presence of cells with intensely eosinophilic cytoplasm, hyperchromatic nuclei and loss of intercellular junctions (Figure 1). The morphologic changes were also observed in the urothelium of the BOV+E group, but the atrophic phenomenon was present in a lesser form than those observed in the BOV group. Interesting in the BOV+E group was the alternation between thickened, hyperplastic areas of urothelium and of normal-looking urothelium were noticed.


Cell proliferation and apoptosis in urinary bladder urothelium of rats following ovariectomy and chronic estrogen replacement therapy.

Lucan L, Stamatian F, Lucan V, Tabaran AF - Clujul Med (2013)

Histopathological findings and immune expression of PCNA and Caspase 3 in studied groups. On the first line of images arrows are indicating the apoptotic cells and the atrophy of the urothelium. In the second and third lines arrows indicates the immunoexpresion for PCNA and Caspase 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4462479&req=5

f1-cm-86-27: Histopathological findings and immune expression of PCNA and Caspase 3 in studied groups. On the first line of images arrows are indicating the apoptotic cells and the atrophy of the urothelium. In the second and third lines arrows indicates the immunoexpresion for PCNA and Caspase 3.
Mentions: The histopathological aspect observed in the urothelium of the BOV group was the marked atrophy, aspect suggested by the decreased thickness of urothelium, presence of cells with intensely eosinophilic cytoplasm, hyperchromatic nuclei and loss of intercellular junctions (Figure 1). The morphologic changes were also observed in the urothelium of the BOV+E group, but the atrophic phenomenon was present in a lesser form than those observed in the BOV group. Interesting in the BOV+E group was the alternation between thickened, hyperplastic areas of urothelium and of normal-looking urothelium were noticed.

Bottom Line: Proliferative and apoptotic activity was monitored by quantifying the urothelium imunoexpression for PCNA antigen as a marker of S phase of the cell cycle and Cleaved Caspase 3 for monitoring apoptotic activity.Estrogen therapy managed to improve the urothelium activity by reducing the Apoptotic Index and by increasing urothelial proliferative activity.The results show the important role of estrogens in maintaining urothelial activities, highlighting their potential use in the treatment of urothelium atrophic and degenerative processes associated with menopause.

View Article: PubMed Central - PubMed

Affiliation: 1 Gynecology Clinic, Iuliu HaĊ£ieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.

ABSTRACT

Aims: In this study we followed the effect of menopause and estrogenic replacement therapy on the proliferative and apoptotic activity of the bladder urothelial cells.

Methods: The experimental model of menopause was reproduced using a standard protocol of bilateral ovariectomy in rats, estrogen replacement therapy being achieved by systemic administration of hexestrol diacetate for six weeks. Proliferative and apoptotic activity was monitored by quantifying the urothelium imunoexpression for PCNA antigen as a marker of S phase of the cell cycle and Cleaved Caspase 3 for monitoring apoptotic activity.

Results: Following ovariectomy, the main changes were urothelial atrophy associated with intensification of the apoptotic activity at these level. Estrogen therapy managed to improve the urothelium activity by reducing the Apoptotic Index and by increasing urothelial proliferative activity.

Conclusions: The results show the important role of estrogens in maintaining urothelial activities, highlighting their potential use in the treatment of urothelium atrophic and degenerative processes associated with menopause.

No MeSH data available.


Related in: MedlinePlus