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SS18-SSX-regulated miR-17 promotes tumor growth of synovial sarcoma by inhibiting p21WAF1/CIP1.

Minami Y, Kohsaka S, Tsuda M, Yachi K, Hatori N, Tanino M, Kimura T, Nishihara H, Minami A, Iwasaki N, Tanaka S - Cancer Sci. (2014)

Bottom Line: Tumor volume formed in mice in vivo was significantly increased by miR-17 overexpression with a marked increase of MIB-1 index.Indeed, p21 protein level was remarkably decreased by miR-17 overexpression in a p53-independent manner.Meanwhile, introduction of anti-miR-17 in Fuji and HS-SYII cells significantly decreased cell growth, consistent with rescued expression of p21.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Japan; Department of Orthopaedic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

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MiR-17 increases growth of synovial sarcoma cells. (a) Fuji and HS-SYII cells were infected with miR-17-producing or its control lentivirus, and the expression levels of miR-17 were examined by semi-quantitative RT-PCR. (b) Proliferation of Fuji and HS-SYII cells with or without MiR-17 overexpression was investigated. *P < 0.05 versus control cells. (c) Colony formation assay was performed in both Fuji and HS-SYII cells with or without miR-17 overexpression. The numbers and size were measured and graphed. ND means not detected. (d) Wound healing assay. Moved distances of Fuji and HS-SYII cells with or without miR-17 overexpression were measured at indicated time points. (e) Matrigel invasion assay. Invaded cells under the filter were counted and graphed.
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fig02: MiR-17 increases growth of synovial sarcoma cells. (a) Fuji and HS-SYII cells were infected with miR-17-producing or its control lentivirus, and the expression levels of miR-17 were examined by semi-quantitative RT-PCR. (b) Proliferation of Fuji and HS-SYII cells with or without MiR-17 overexpression was investigated. *P < 0.05 versus control cells. (c) Colony formation assay was performed in both Fuji and HS-SYII cells with or without miR-17 overexpression. The numbers and size were measured and graphed. ND means not detected. (d) Wound healing assay. Moved distances of Fuji and HS-SYII cells with or without miR-17 overexpression were measured at indicated time points. (e) Matrigel invasion assay. Invaded cells under the filter were counted and graphed.

Mentions: To clarify the functions of miR-17 in synovial sarcoma cells, we stably established miR-17-overexpressing Fuji and HS-SYII cells by infecting the cells with miR-17-producing lentivirus (Fig.2a). MiR-17 overexpression significantly promoted cell proliferation in both Fuji and HS-SYII cells, reaching 1.6-fold and 4.0-fold increases compared to the control cells, respectively (Fig.2b). In addition, forced expression of miR-17 in Fuji and HS-SYII cells increased the colony formation ability compared to the corresponding control cells (Fig.2c). Based on these findings, miR-17 might have a similar biological effect on cell proliferation and colony formation both in SS18-SSX1 and SS18-SSX2-harboring synovial sarcoma cells. In contrast, cell motility and invasion capabilities were not altered by miR-17 overexpression (Fig.2d,e). MiR-17 overexpression did not lead to significant alterations on the cell morphologies (data not shown).


SS18-SSX-regulated miR-17 promotes tumor growth of synovial sarcoma by inhibiting p21WAF1/CIP1.

Minami Y, Kohsaka S, Tsuda M, Yachi K, Hatori N, Tanino M, Kimura T, Nishihara H, Minami A, Iwasaki N, Tanaka S - Cancer Sci. (2014)

MiR-17 increases growth of synovial sarcoma cells. (a) Fuji and HS-SYII cells were infected with miR-17-producing or its control lentivirus, and the expression levels of miR-17 were examined by semi-quantitative RT-PCR. (b) Proliferation of Fuji and HS-SYII cells with or without MiR-17 overexpression was investigated. *P < 0.05 versus control cells. (c) Colony formation assay was performed in both Fuji and HS-SYII cells with or without miR-17 overexpression. The numbers and size were measured and graphed. ND means not detected. (d) Wound healing assay. Moved distances of Fuji and HS-SYII cells with or without miR-17 overexpression were measured at indicated time points. (e) Matrigel invasion assay. Invaded cells under the filter were counted and graphed.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4462386&req=5

fig02: MiR-17 increases growth of synovial sarcoma cells. (a) Fuji and HS-SYII cells were infected with miR-17-producing or its control lentivirus, and the expression levels of miR-17 were examined by semi-quantitative RT-PCR. (b) Proliferation of Fuji and HS-SYII cells with or without MiR-17 overexpression was investigated. *P < 0.05 versus control cells. (c) Colony formation assay was performed in both Fuji and HS-SYII cells with or without miR-17 overexpression. The numbers and size were measured and graphed. ND means not detected. (d) Wound healing assay. Moved distances of Fuji and HS-SYII cells with or without miR-17 overexpression were measured at indicated time points. (e) Matrigel invasion assay. Invaded cells under the filter were counted and graphed.
Mentions: To clarify the functions of miR-17 in synovial sarcoma cells, we stably established miR-17-overexpressing Fuji and HS-SYII cells by infecting the cells with miR-17-producing lentivirus (Fig.2a). MiR-17 overexpression significantly promoted cell proliferation in both Fuji and HS-SYII cells, reaching 1.6-fold and 4.0-fold increases compared to the control cells, respectively (Fig.2b). In addition, forced expression of miR-17 in Fuji and HS-SYII cells increased the colony formation ability compared to the corresponding control cells (Fig.2c). Based on these findings, miR-17 might have a similar biological effect on cell proliferation and colony formation both in SS18-SSX1 and SS18-SSX2-harboring synovial sarcoma cells. In contrast, cell motility and invasion capabilities were not altered by miR-17 overexpression (Fig.2d,e). MiR-17 overexpression did not lead to significant alterations on the cell morphologies (data not shown).

Bottom Line: Tumor volume formed in mice in vivo was significantly increased by miR-17 overexpression with a marked increase of MIB-1 index.Indeed, p21 protein level was remarkably decreased by miR-17 overexpression in a p53-independent manner.Meanwhile, introduction of anti-miR-17 in Fuji and HS-SYII cells significantly decreased cell growth, consistent with rescued expression of p21.

View Article: PubMed Central - PubMed

Affiliation: Department of Cancer Pathology, Hokkaido University Graduate School of Medicine, Sapporo, Japan; Department of Orthopaedic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

Show MeSH
Related in: MedlinePlus