Intratumoral androgen metabolism and actions in invasive lobular carcinoma of the breast.
Bottom Line: Invasive lobular carcinoma (ILC) accounts for approximately 10% of all breast carcinomas and is characterized by higher levels of androgen receptor (AR) compared to invasive ductal carcinoma (IDC).In ILC cases the status of 5αRed1 and 17βHSD5 was inversely correlated with tumor size (P = 0.0053) and nuclear grade (P = 0.0290), and significantly associated with better overall survival of the patients (P = 0.0059).Results of our present study indicated that androgen-producing enzymes could play pivotal protective roles in AR-enriched ILC cases.
Affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai.Show MeSH
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Mentions: It was previously reported that 17╬▓HSD2 reflected intratumoral androgenic actions in breast cancer.(27) In this study, 17╬▓HSD2 mRNA levels were quantified in the four ILC and four IDC cases summarized in Table 1 with RT2 qPCR, in order to compare 17╬▓HSD2 mRNA expression between ILC and IDC cases. As illustrated in Figure 4(a), the amount of 17╬▓HSD2 mRNA expression was 4.8-fold higher in ILC than IDC cases (P = 0.0775). The 17╬▓HSD2 immunoreactivity was also examined in order to further explore the significance of this particular enzyme with relation to androgen actions. Fifty-four IDC cases (Tohoku University Hospital) were selected so that ER, PR, Her2, Ki-67, stage, AR, 17╬▓HSD5, and 5╬▒Red1 status did not differ significantly with 46 ILC cases (Tohoku University Hospital). Immunoreactivity of 17╬▓HSD2 was detected in the cytoplasm of carcinoma cells (Fig. 4b). The number of 17╬▓HSD2-positive cases was significantly higher in ILC than in IDC despite nearly the same status of hormone receptors between ILC and IDC cases examined (Fig. 4c).
Affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai.