Intratumoral androgen metabolism and actions in invasive lobular carcinoma of the breast.
Bottom Line: In ILC cases the status of 5αRed1 and 17βHSD5 was inversely correlated with tumor size (P = 0.0053) and nuclear grade (P = 0.0290), and significantly associated with better overall survival of the patients (P = 0.0059).These correlations suggest one protective mode of androgen action could be through modulation of estrogen metabolism.Results of our present study indicated that androgen-producing enzymes could play pivotal protective roles in AR-enriched ILC cases.
Affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai.Show MeSH
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Mentions: It was previously reported that 17╬▓HSD2 reflected intratumoral androgenic actions in breast cancer.(27) In this study, 17╬▓HSD2 mRNA levels were quantified in the four ILC and four IDC cases summarized in Table 1 with RT2 qPCR, in order to compare 17╬▓HSD2 mRNA expression between ILC and IDC cases. As illustrated in Figure 4(a), the amount of 17╬▓HSD2 mRNA expression was 4.8-fold higher in ILC than IDC cases (P = 0.0775). The 17╬▓HSD2 immunoreactivity was also examined in order to further explore the significance of this particular enzyme with relation to androgen actions. Fifty-four IDC cases (Tohoku University Hospital) were selected so that ER, PR, Her2, Ki-67, stage, AR, 17╬▓HSD5, and 5╬▒Red1 status did not differ significantly with 46 ILC cases (Tohoku University Hospital). Immunoreactivity of 17╬▓HSD2 was detected in the cytoplasm of carcinoma cells (Fig. 4b). The number of 17╬▓HSD2-positive cases was significantly higher in ILC than in IDC despite nearly the same status of hormone receptors between ILC and IDC cases examined (Fig. 4c).
Affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai.