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Inhibition of metastasis of rhabdomyosarcoma by a novel neutralizing antibody to CXC chemokine receptor-4.

Kashima K, Watanabe M, Sato Y, Hata J, Ishii N, Aoki Y - Cancer Sci. (2014)

Bottom Line: In addition, CF172 was found to inhibit the SDF1-induced migration activity of SJCRH30 cells in vitro.These studies indicated that CF172 significantly decreased both types of metastasis of SJCRH30.In conclusion, we found that a novel anti-CXCR4 mAb, CF172, with specific reactivity against human CXCR4, prevented peritoneal metastasis and lymph node metastasis of rhabdomyosarcoma in animal models.

View Article: PubMed Central - PubMed

Affiliation: Research Division, Chugai Pharmaceutical Co. Ltd., Kanagawa, Japan.

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Analysis of CXC chemokine receptor-4 (CXCR4) expression in rhabdomyosarcoma cell lines. (a) CXCR4 membrane expression in rhabdomyo-sarcoma cell lines was analyzed by flow cytometry using CF172. Background binding, measured with isotype control (solid area), is also shown. (b) Expression of CXCR4 protein in five rhabdomyo-sarcoma cell lines was analyzed by Western blotting using anti-CXCR4 antibody.
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fig03: Analysis of CXC chemokine receptor-4 (CXCR4) expression in rhabdomyosarcoma cell lines. (a) CXCR4 membrane expression in rhabdomyo-sarcoma cell lines was analyzed by flow cytometry using CF172. Background binding, measured with isotype control (solid area), is also shown. (b) Expression of CXCR4 protein in five rhabdomyo-sarcoma cell lines was analyzed by Western blotting using anti-CXCR4 antibody.

Mentions: Next, to investigate CXCR4 expression on the cell surface of rhabdomyosarcoma cells, we carried out FACS assays of CF172 using five different rhabdomyosarcoma cell lines. We found that SJCRH30 and RH30 cells expressed human CXCR4, and the expression levels of SJCRH30 were higher than those of RH30 (Fig.3a). To confirm this expression pattern, we used Western blot analysis (Fig.3b) and real-time PCR (Fig. S1). Consistent with the cell surface expression pattern (Fig.3a), SJCRH30 showed the highest mRNA/protein expression for CXCR4 among the five rhabdomyosarcoma cell lines.


Inhibition of metastasis of rhabdomyosarcoma by a novel neutralizing antibody to CXC chemokine receptor-4.

Kashima K, Watanabe M, Sato Y, Hata J, Ishii N, Aoki Y - Cancer Sci. (2014)

Analysis of CXC chemokine receptor-4 (CXCR4) expression in rhabdomyosarcoma cell lines. (a) CXCR4 membrane expression in rhabdomyo-sarcoma cell lines was analyzed by flow cytometry using CF172. Background binding, measured with isotype control (solid area), is also shown. (b) Expression of CXCR4 protein in five rhabdomyo-sarcoma cell lines was analyzed by Western blotting using anti-CXCR4 antibody.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4462355&req=5

fig03: Analysis of CXC chemokine receptor-4 (CXCR4) expression in rhabdomyosarcoma cell lines. (a) CXCR4 membrane expression in rhabdomyo-sarcoma cell lines was analyzed by flow cytometry using CF172. Background binding, measured with isotype control (solid area), is also shown. (b) Expression of CXCR4 protein in five rhabdomyo-sarcoma cell lines was analyzed by Western blotting using anti-CXCR4 antibody.
Mentions: Next, to investigate CXCR4 expression on the cell surface of rhabdomyosarcoma cells, we carried out FACS assays of CF172 using five different rhabdomyosarcoma cell lines. We found that SJCRH30 and RH30 cells expressed human CXCR4, and the expression levels of SJCRH30 were higher than those of RH30 (Fig.3a). To confirm this expression pattern, we used Western blot analysis (Fig.3b) and real-time PCR (Fig. S1). Consistent with the cell surface expression pattern (Fig.3a), SJCRH30 showed the highest mRNA/protein expression for CXCR4 among the five rhabdomyosarcoma cell lines.

Bottom Line: In addition, CF172 was found to inhibit the SDF1-induced migration activity of SJCRH30 cells in vitro.These studies indicated that CF172 significantly decreased both types of metastasis of SJCRH30.In conclusion, we found that a novel anti-CXCR4 mAb, CF172, with specific reactivity against human CXCR4, prevented peritoneal metastasis and lymph node metastasis of rhabdomyosarcoma in animal models.

View Article: PubMed Central - PubMed

Affiliation: Research Division, Chugai Pharmaceutical Co. Ltd., Kanagawa, Japan.

Show MeSH
Related in: MedlinePlus