Inhibition of metastasis of rhabdomyosarcoma by a novel neutralizing antibody to CXC chemokine receptor-4.
Bottom Line: In addition, CF172 was found to inhibit the SDF1-induced migration activity of SJCRH30 cells in vitro.These studies indicated that CF172 significantly decreased both types of metastasis of SJCRH30.In conclusion, we found that a novel anti-CXCR4 mAb, CF172, with specific reactivity against human CXCR4, prevented peritoneal metastasis and lymph node metastasis of rhabdomyosarcoma in animal models.
Affiliation: Research Division, Chugai Pharmaceutical Co. Ltd., Kanagawa, Japan.Show MeSH
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Mentions: The binding activity of CF172 to human CXCR4 was determined by FACS using human CXCR4-expressing CHO cells (Fig.1a). To evaluate the binding specificity of CF172 against human CXCR4, we carried out the FACS assay using CHO cells expressing mouse CXCR4 and human CXCR2 (the protein most closely related to CXCR4). Results of the assay indicated that CF172 did not bind to either mouse CXCR4- or human CXCR2-expressing CHO cells (Fig.1b,c, respectively).
Affiliation: Research Division, Chugai Pharmaceutical Co. Ltd., Kanagawa, Japan.