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Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor.

Wang VB, Sivakumar K, Yang L, Zhang Q, Kjelleberg S, Loo SC, Cao B - Sci Rep (2015)

Bottom Line: Our results show that the transfer of formate is the key mechanism in this mutualistic system.More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device.RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.

View Article: PubMed Central - PubMed

Affiliation: 1] School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore [2] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore.

ABSTRACT
Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.

No MeSH data available.


Related in: MedlinePlus

Electrical output of MFCs inoculated with mono- and co-cultures of E. coli and S. oneidensis microbial strains.(a) Current density generated as a function of time. (b) Maximum current density (average ± standard deviation). Data represent the average of triplicates.
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f1: Electrical output of MFCs inoculated with mono- and co-cultures of E. coli and S. oneidensis microbial strains.(a) Current density generated as a function of time. (b) Maximum current density (average ± standard deviation). Data represent the average of triplicates.

Mentions: To test the mutualistic co-culture relationship between wild type E. coli and S. oneidensis strains, MFCs were employed as a platform to determine the extent of extracellular electron transfer in terms of electrical output in bioelectrochemical devices, with both species inoculated into the anode chamber. MFCs were operated for each experimental parameter and the average generated current densities by each of the combination of wild type or mutant strains of S. oneidensis were recorded (Fig. 1a). The co-culture MFCs produced a significantly higher average maximum current density (~2.0 μA/cm2), whereas mono-culture MFCs produced negligible current densities (Fig. 1b). These findings suggest that there were mutualistic interactions between E. coli and S. oneidensis. Further, the experiment revealed a rapid increase (~0.17 μA/cm2/h) in the average current density generated by the co-culture, suggesting an immediate establishment of the mutualistic interaction.


Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor.

Wang VB, Sivakumar K, Yang L, Zhang Q, Kjelleberg S, Loo SC, Cao B - Sci Rep (2015)

Electrical output of MFCs inoculated with mono- and co-cultures of E. coli and S. oneidensis microbial strains.(a) Current density generated as a function of time. (b) Maximum current density (average ± standard deviation). Data represent the average of triplicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4462164&req=5

f1: Electrical output of MFCs inoculated with mono- and co-cultures of E. coli and S. oneidensis microbial strains.(a) Current density generated as a function of time. (b) Maximum current density (average ± standard deviation). Data represent the average of triplicates.
Mentions: To test the mutualistic co-culture relationship between wild type E. coli and S. oneidensis strains, MFCs were employed as a platform to determine the extent of extracellular electron transfer in terms of electrical output in bioelectrochemical devices, with both species inoculated into the anode chamber. MFCs were operated for each experimental parameter and the average generated current densities by each of the combination of wild type or mutant strains of S. oneidensis were recorded (Fig. 1a). The co-culture MFCs produced a significantly higher average maximum current density (~2.0 μA/cm2), whereas mono-culture MFCs produced negligible current densities (Fig. 1b). These findings suggest that there were mutualistic interactions between E. coli and S. oneidensis. Further, the experiment revealed a rapid increase (~0.17 μA/cm2/h) in the average current density generated by the co-culture, suggesting an immediate establishment of the mutualistic interaction.

Bottom Line: Our results show that the transfer of formate is the key mechanism in this mutualistic system.More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device.RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.

View Article: PubMed Central - PubMed

Affiliation: 1] School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore [2] Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore 637551, Singapore.

ABSTRACT
Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.

No MeSH data available.


Related in: MedlinePlus