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SERS Detection of Dopamine Using Label-Free Acridine Red as Molecular Probe in Reduced Graphene Oxide/Silver Nanotriangle Sol Substrate.

Luo Y, Ma L, Zhang X, Liang A, Jiang Z - Nanoscale Res Lett (2015)

Bottom Line: The reduced graphene oxide/silver nanotriangle (rGO/AgNT) composite sol was prepared by the reduction of silver ions with sodium borohydride in the presence of H2O2 and sodium citrate.Upon addition of dopamine (DA), the competitive adsorption between DA and AR with the rGO took place, and the AR molecules were adsorbed on the AgNT aggregates with a strong SERS peak at 1506 cm(-1) that caused the SERS peak increase.The increased SERS intensity is linear to the DA concentration in the range of 2.5-500 μmol/L.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China, 250581250@qq.com.

ABSTRACT
The reduced graphene oxide/silver nanotriangle (rGO/AgNT) composite sol was prepared by the reduction of silver ions with sodium borohydride in the presence of H2O2 and sodium citrate. In the nanosol substrate, the molecular probe of acridine red (AR) exhibited a weak surface-enhanced Raman scattering (SERS) peak at 1506 cm(-1) due to its interaction with the rGO of rGO/AgNT. Upon addition of dopamine (DA), the competitive adsorption between DA and AR with the rGO took place, and the AR molecules were adsorbed on the AgNT aggregates with a strong SERS peak at 1506 cm(-1) that caused the SERS peak increase. The increased SERS intensity is linear to the DA concentration in the range of 2.5-500 μmol/L. This new analytical system was investigated by SERS, fluorescence, absorption, transmission electron microscope (TEM), and scanning electron microscope (SEM) techniques, and a SERS quantitative analysis method for DA was established, using AR as a label-free molecular probe.

No MeSH data available.


Related in: MedlinePlus

Effect of pH. 1.75×10−6 mol/L AR-PBS-2.5×10−5 mol/L DA-0.05 mol/L NaCl-5.39 μg/mL rGO/AgNT
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Fig8: Effect of pH. 1.75×10−6 mol/L AR-PBS-2.5×10−5 mol/L DA-0.05 mol/L NaCl-5.39 μg/mL rGO/AgNT

Mentions: The effect of pH for the PBS buffer solution was studied (Fig. 8). The ΔI value reached its maximum when the pH was 6.0. Thus, a pH 6.0 PBS buffer solution was selected for use. The effect of AR concentration on the determination was studied (Fig. 9). When the concentration of rGO held constant, as the concentration of AR increased, ΔI was increased as well. The concentration of AR was more than 1.75×10−6 mol/L; the SERS signal of the system without DA increased due to excess AR absorbed on the AgNT aggregates that resulted in the decrease of ΔI. Thus, a 1.75×10−6 mol/L AR was chosen for the assay. NaCl was a good aggregation reagent for AgNT. The effect of NaCl concentration on the determination was studied (Fig. 10). As the concentration of NaCl increased, ΔI was enhanced as well. When the concentration of NaCl was 0.04 mol/L, the ΔI value reached the maximum. When the concentration of NaCl increased further, the ΔI value decreased because the AgNT aggregated excessively and precipitated. Thus, a 0.04 mol/L NaCl was chosen for the assay. The influence of the rGO/AgNT concentration on the determination was studied (Fig. 11). When the concentration of rGO/AgNT was 8.62 μg/mL, the ΔI value was maximal. Thus, a 8.62 μg/mL rGO/AgNT was chosen for the assay.Fig. 8


SERS Detection of Dopamine Using Label-Free Acridine Red as Molecular Probe in Reduced Graphene Oxide/Silver Nanotriangle Sol Substrate.

Luo Y, Ma L, Zhang X, Liang A, Jiang Z - Nanoscale Res Lett (2015)

Effect of pH. 1.75×10−6 mol/L AR-PBS-2.5×10−5 mol/L DA-0.05 mol/L NaCl-5.39 μg/mL rGO/AgNT
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4457732&req=5

Fig8: Effect of pH. 1.75×10−6 mol/L AR-PBS-2.5×10−5 mol/L DA-0.05 mol/L NaCl-5.39 μg/mL rGO/AgNT
Mentions: The effect of pH for the PBS buffer solution was studied (Fig. 8). The ΔI value reached its maximum when the pH was 6.0. Thus, a pH 6.0 PBS buffer solution was selected for use. The effect of AR concentration on the determination was studied (Fig. 9). When the concentration of rGO held constant, as the concentration of AR increased, ΔI was increased as well. The concentration of AR was more than 1.75×10−6 mol/L; the SERS signal of the system without DA increased due to excess AR absorbed on the AgNT aggregates that resulted in the decrease of ΔI. Thus, a 1.75×10−6 mol/L AR was chosen for the assay. NaCl was a good aggregation reagent for AgNT. The effect of NaCl concentration on the determination was studied (Fig. 10). As the concentration of NaCl increased, ΔI was enhanced as well. When the concentration of NaCl was 0.04 mol/L, the ΔI value reached the maximum. When the concentration of NaCl increased further, the ΔI value decreased because the AgNT aggregated excessively and precipitated. Thus, a 0.04 mol/L NaCl was chosen for the assay. The influence of the rGO/AgNT concentration on the determination was studied (Fig. 11). When the concentration of rGO/AgNT was 8.62 μg/mL, the ΔI value was maximal. Thus, a 8.62 μg/mL rGO/AgNT was chosen for the assay.Fig. 8

Bottom Line: The reduced graphene oxide/silver nanotriangle (rGO/AgNT) composite sol was prepared by the reduction of silver ions with sodium borohydride in the presence of H2O2 and sodium citrate.Upon addition of dopamine (DA), the competitive adsorption between DA and AR with the rGO took place, and the AR molecules were adsorbed on the AgNT aggregates with a strong SERS peak at 1506 cm(-1) that caused the SERS peak increase.The increased SERS intensity is linear to the DA concentration in the range of 2.5-500 μmol/L.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China, 250581250@qq.com.

ABSTRACT
The reduced graphene oxide/silver nanotriangle (rGO/AgNT) composite sol was prepared by the reduction of silver ions with sodium borohydride in the presence of H2O2 and sodium citrate. In the nanosol substrate, the molecular probe of acridine red (AR) exhibited a weak surface-enhanced Raman scattering (SERS) peak at 1506 cm(-1) due to its interaction with the rGO of rGO/AgNT. Upon addition of dopamine (DA), the competitive adsorption between DA and AR with the rGO took place, and the AR molecules were adsorbed on the AgNT aggregates with a strong SERS peak at 1506 cm(-1) that caused the SERS peak increase. The increased SERS intensity is linear to the DA concentration in the range of 2.5-500 μmol/L. This new analytical system was investigated by SERS, fluorescence, absorption, transmission electron microscope (TEM), and scanning electron microscope (SEM) techniques, and a SERS quantitative analysis method for DA was established, using AR as a label-free molecular probe.

No MeSH data available.


Related in: MedlinePlus