Limits...
TaER Expression Is Associated with Transpiration Efficiency Traits and Yield in Bread Wheat.

Zheng J, Yang Z, Madgwick PJ, Carmo-Silva E, Parry MA, Hu YG - PLoS ONE (2015)

Bottom Line: Correlation analysis revealed that the expression of TaER1 and TaER2 at the two growth stages was significantly and negatively associated with SD (P<0.01), transpiration rate (P<0.05) and CID (P<0.01), while significantly and positively correlated with flag leaf area (FLA, P<0.01), A (P<0.05), WUEi (P<0.05), BYPP (P<0.01) and GYPP (P<0.01), with stronger correlations for TaER1 than TaER2 and at grain-filling stage than at heading stage.These combined results suggested that TaER involved in development of transpiration efficiency -related traits and yield in bread wheat, implying a function for TaER in regulating leaf development of bread wheat and contributing to expression of these traits.Moreover, the results indicate that TaER could be exploitable for manipulating important agronomical traits in wheat improvement.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
ERECTA encodes a receptor-like kinase and is proposed as a candidate for determining transpiration efficiency of plants. Two genes homologous to ERECTA in Arabidopsis were identified on chromosomes 6 (TaER2) and 7 (TaER1) of bread wheat (Triticum aestivum L.), with copies of each gene on the A, B and D genomes of wheat. Similar expression patterns were observed for TaER1 and TaER2 with relatively higher expression of TaER1 in flag leaves of wheat at heading (Z55) and grain-filling (Z73) stages. Significant variations were found in the expression levels of both TaER1 and TaER2 in the flag leaves at both growth stages among 48 diverse bread wheat varieties. Based on the expression of TaER1 and TaER2, the 48 wheat varieties could be classified into three groups having high (5 varieties), medium (27 varieties) and low (16 varieties) levels of TaER expression. Significant differences were also observed between the three groups varying for TaER expression for several transpiration efficiency (TE)- related traits, including stomatal density (SD), transpiration rate, photosynthetic rate (A), instant water use efficiency (WUEi) and carbon isotope discrimination (CID), and yield traits of biomass production plant-1 (BYPP) and grain yield plant-1 (GYPP). Correlation analysis revealed that the expression of TaER1 and TaER2 at the two growth stages was significantly and negatively associated with SD (P<0.01), transpiration rate (P<0.05) and CID (P<0.01), while significantly and positively correlated with flag leaf area (FLA, P<0.01), A (P<0.05), WUEi (P<0.05), BYPP (P<0.01) and GYPP (P<0.01), with stronger correlations for TaER1 than TaER2 and at grain-filling stage than at heading stage. These combined results suggested that TaER involved in development of transpiration efficiency -related traits and yield in bread wheat, implying a function for TaER in regulating leaf development of bread wheat and contributing to expression of these traits. Moreover, the results indicate that TaER could be exploitable for manipulating important agronomical traits in wheat improvement.

No MeSH data available.


Related in: MedlinePlus

Relative expression of TaER1 and TaER2 in flag leaves of 48 wheat genotypes at heading (Z55) and grain-filling (Z73) stage.Group I: high TaER expression; Group II: intermediate TaER expression; Group III: low TaER expression. Uppercase letters represent significant differences among the three groups (P<0.01). The reference genes were TaActin, TaCell and TaSand. Values are presented with the mean ± SD calculated from the formula [13, 14, 15]:NE=(EX)-Ct,X(ER)-Ct,R
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4457575&req=5

pone.0128415.g003: Relative expression of TaER1 and TaER2 in flag leaves of 48 wheat genotypes at heading (Z55) and grain-filling (Z73) stage.Group I: high TaER expression; Group II: intermediate TaER expression; Group III: low TaER expression. Uppercase letters represent significant differences among the three groups (P<0.01). The reference genes were TaActin, TaCell and TaSand. Values are presented with the mean ± SD calculated from the formula [13, 14, 15]:NE=(EX)-Ct,X(ER)-Ct,R

Mentions: The expression levels of TaER1 and TaER2 in wheat flag leaves were higher at heading (Z55) stage than at grain-filling (Z73) stage, with similar expression patterns in both TaER1 and TaER2 at the two stages (Fig 3, S3 Table). Significant variations were observed on the expression levels of TaER1 and TaER2 in the flag leaves at both stages among the 48 diverse bread wheat varieties (Fig 3, S3 Table). Cluster analysis on the expression levels of two TaER genes, classified these varieties into three groups as: 5 varieties with high TaER expression in group I, 27 varieties with intermediate TaER expression in group II and 16 varieties with low TaER expression in group III (Fig 4, S3 Table). The distribution of genotypes per group implied that there were relatively few genotypes with higher TaER expressions, but most genotypes with intermediate TaER expression across these wheat varieties.


TaER Expression Is Associated with Transpiration Efficiency Traits and Yield in Bread Wheat.

Zheng J, Yang Z, Madgwick PJ, Carmo-Silva E, Parry MA, Hu YG - PLoS ONE (2015)

Relative expression of TaER1 and TaER2 in flag leaves of 48 wheat genotypes at heading (Z55) and grain-filling (Z73) stage.Group I: high TaER expression; Group II: intermediate TaER expression; Group III: low TaER expression. Uppercase letters represent significant differences among the three groups (P<0.01). The reference genes were TaActin, TaCell and TaSand. Values are presented with the mean ± SD calculated from the formula [13, 14, 15]:NE=(EX)-Ct,X(ER)-Ct,R
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4457575&req=5

pone.0128415.g003: Relative expression of TaER1 and TaER2 in flag leaves of 48 wheat genotypes at heading (Z55) and grain-filling (Z73) stage.Group I: high TaER expression; Group II: intermediate TaER expression; Group III: low TaER expression. Uppercase letters represent significant differences among the three groups (P<0.01). The reference genes were TaActin, TaCell and TaSand. Values are presented with the mean ± SD calculated from the formula [13, 14, 15]:NE=(EX)-Ct,X(ER)-Ct,R
Mentions: The expression levels of TaER1 and TaER2 in wheat flag leaves were higher at heading (Z55) stage than at grain-filling (Z73) stage, with similar expression patterns in both TaER1 and TaER2 at the two stages (Fig 3, S3 Table). Significant variations were observed on the expression levels of TaER1 and TaER2 in the flag leaves at both stages among the 48 diverse bread wheat varieties (Fig 3, S3 Table). Cluster analysis on the expression levels of two TaER genes, classified these varieties into three groups as: 5 varieties with high TaER expression in group I, 27 varieties with intermediate TaER expression in group II and 16 varieties with low TaER expression in group III (Fig 4, S3 Table). The distribution of genotypes per group implied that there were relatively few genotypes with higher TaER expressions, but most genotypes with intermediate TaER expression across these wheat varieties.

Bottom Line: Correlation analysis revealed that the expression of TaER1 and TaER2 at the two growth stages was significantly and negatively associated with SD (P<0.01), transpiration rate (P<0.05) and CID (P<0.01), while significantly and positively correlated with flag leaf area (FLA, P<0.01), A (P<0.05), WUEi (P<0.05), BYPP (P<0.01) and GYPP (P<0.01), with stronger correlations for TaER1 than TaER2 and at grain-filling stage than at heading stage.These combined results suggested that TaER involved in development of transpiration efficiency -related traits and yield in bread wheat, implying a function for TaER in regulating leaf development of bread wheat and contributing to expression of these traits.Moreover, the results indicate that TaER could be exploitable for manipulating important agronomical traits in wheat improvement.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

ABSTRACT
ERECTA encodes a receptor-like kinase and is proposed as a candidate for determining transpiration efficiency of plants. Two genes homologous to ERECTA in Arabidopsis were identified on chromosomes 6 (TaER2) and 7 (TaER1) of bread wheat (Triticum aestivum L.), with copies of each gene on the A, B and D genomes of wheat. Similar expression patterns were observed for TaER1 and TaER2 with relatively higher expression of TaER1 in flag leaves of wheat at heading (Z55) and grain-filling (Z73) stages. Significant variations were found in the expression levels of both TaER1 and TaER2 in the flag leaves at both growth stages among 48 diverse bread wheat varieties. Based on the expression of TaER1 and TaER2, the 48 wheat varieties could be classified into three groups having high (5 varieties), medium (27 varieties) and low (16 varieties) levels of TaER expression. Significant differences were also observed between the three groups varying for TaER expression for several transpiration efficiency (TE)- related traits, including stomatal density (SD), transpiration rate, photosynthetic rate (A), instant water use efficiency (WUEi) and carbon isotope discrimination (CID), and yield traits of biomass production plant-1 (BYPP) and grain yield plant-1 (GYPP). Correlation analysis revealed that the expression of TaER1 and TaER2 at the two growth stages was significantly and negatively associated with SD (P<0.01), transpiration rate (P<0.05) and CID (P<0.01), while significantly and positively correlated with flag leaf area (FLA, P<0.01), A (P<0.05), WUEi (P<0.05), BYPP (P<0.01) and GYPP (P<0.01), with stronger correlations for TaER1 than TaER2 and at grain-filling stage than at heading stage. These combined results suggested that TaER involved in development of transpiration efficiency -related traits and yield in bread wheat, implying a function for TaER in regulating leaf development of bread wheat and contributing to expression of these traits. Moreover, the results indicate that TaER could be exploitable for manipulating important agronomical traits in wheat improvement.

No MeSH data available.


Related in: MedlinePlus