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The prognostic significance of fibroblast growth factor receptor 4 in non-small-cell lung cancer.

Huang HP, Feng H, Qiao HB, Ren ZX, Zhu GD - Onco Targets Ther (2015)

Bottom Line: Fibroblast growth factor receptor 4 (FGFR4) has been proved to be correlated with progression and prognosis in many cancers.Moreover, we evaluated the prognostic value of FGFR4 by Kaplan-Meier survival curve and Cox regression model.FGFR4 expression was significantly associated with tumor diameter (P=0.039).

View Article: PubMed Central - PubMed

Affiliation: Department of General Medicine, Linyi Hospital Affiliated to Shandong University, Linyi City, People's Republic of China.

ABSTRACT

Background: Fibroblast growth factor receptor 4 (FGFR4) has been proved to be correlated with progression and prognosis in many cancers. However, the significance of FGFR4 in non-small-cell lung cancer (NSCLC) is still not well elucidated.

Methods: In our experiment, we detected FGFR4 expression in 237 samples of NSCLC with immunohistochemistry, and further analyzed the correlation between FGFR4 and clinicopathologic features of NSCLC with chi-square test. Moreover, we evaluated the prognostic value of FGFR4 by Kaplan-Meier survival curve and Cox regression model. By regulating the expression of FGFR4 by overexpression or knockdown, we assessed the role of FGFR4 on NSCLC cell proliferation.

Results: FGFR4 expression was high in NSCLC (46.8%, 111/237). FGFR4 expression was significantly associated with tumor diameter (P=0.039). With univariate (P=0.009) and multivariate (P=0.002) analysis, FGFR4 was identified as an independent prognostic factor in NSCLC (P=0.009). Moreover, FGFR4 can promote the proliferation of NSCLC cell lines.

Conclusion: FGFR4 is an independent prognostic biomarker in NSCLC. FGFR4 can accelerate the proliferation of NSCLC cell lines, indicating FGFR4 could be a potential drug target of NSCLC.

No MeSH data available.


Related in: MedlinePlus

FGFR4 expression in non-small-cell lung cancer.Notes: (A) Lower FGFR4 expression in lung adenocarcinoma. (B) Higher FGFR4 expression in lung adenocarcinoma. (C) Lower FGFR4 expression in squamous cell carcinoma. (D) Higher FGFR4 expression in squamous cell carcinoma. Scale bar: 50 μm. (E) FGFR4 mRNA level in tumor tissue and adjacent normal tissue (n=12).Abbreviation: FGFR4, fibroblast growth factor receptor 4.
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f1-ott-8-1157: FGFR4 expression in non-small-cell lung cancer.Notes: (A) Lower FGFR4 expression in lung adenocarcinoma. (B) Higher FGFR4 expression in lung adenocarcinoma. (C) Lower FGFR4 expression in squamous cell carcinoma. (D) Higher FGFR4 expression in squamous cell carcinoma. Scale bar: 50 μm. (E) FGFR4 mRNA level in tumor tissue and adjacent normal tissue (n=12).Abbreviation: FGFR4, fibroblast growth factor receptor 4.

Mentions: The validation cohort consisted of 237 NSCLC patients. The age of patients ranged from 33 to 78 years old, with median age 60 years (Table 1). Most patients (65%) were male in the validation cohort. The clinicopathologic parameters, including tumor size, lymph node metastasis, histological type, differentiation, and smoking, were according to hospital and surgical records. The cohort was divided into FGFR4 high-expression and low-expression groups according to the IHC criteria described in “Materials and methods”. In our study, FGFR4 expression was mainly observed in both cytoplasm and membrane (Figure 1A–D). Statistically, the rate of higher FGFR4 expression was 46.8% (111/237) in NSCLC. To compare the FGFR4 expression in tumor tissue or adjacent normal tissue, we detected the FGFR4 mRNA level from 12 pairs of frozen samples of NSCLC with quantitative PCR. It turned out that FGFR4 mRNA in tumor tissues was significantly higher than mRNA in normal tissues (Figure 1E).


The prognostic significance of fibroblast growth factor receptor 4 in non-small-cell lung cancer.

Huang HP, Feng H, Qiao HB, Ren ZX, Zhu GD - Onco Targets Ther (2015)

FGFR4 expression in non-small-cell lung cancer.Notes: (A) Lower FGFR4 expression in lung adenocarcinoma. (B) Higher FGFR4 expression in lung adenocarcinoma. (C) Lower FGFR4 expression in squamous cell carcinoma. (D) Higher FGFR4 expression in squamous cell carcinoma. Scale bar: 50 μm. (E) FGFR4 mRNA level in tumor tissue and adjacent normal tissue (n=12).Abbreviation: FGFR4, fibroblast growth factor receptor 4.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4447177&req=5

f1-ott-8-1157: FGFR4 expression in non-small-cell lung cancer.Notes: (A) Lower FGFR4 expression in lung adenocarcinoma. (B) Higher FGFR4 expression in lung adenocarcinoma. (C) Lower FGFR4 expression in squamous cell carcinoma. (D) Higher FGFR4 expression in squamous cell carcinoma. Scale bar: 50 μm. (E) FGFR4 mRNA level in tumor tissue and adjacent normal tissue (n=12).Abbreviation: FGFR4, fibroblast growth factor receptor 4.
Mentions: The validation cohort consisted of 237 NSCLC patients. The age of patients ranged from 33 to 78 years old, with median age 60 years (Table 1). Most patients (65%) were male in the validation cohort. The clinicopathologic parameters, including tumor size, lymph node metastasis, histological type, differentiation, and smoking, were according to hospital and surgical records. The cohort was divided into FGFR4 high-expression and low-expression groups according to the IHC criteria described in “Materials and methods”. In our study, FGFR4 expression was mainly observed in both cytoplasm and membrane (Figure 1A–D). Statistically, the rate of higher FGFR4 expression was 46.8% (111/237) in NSCLC. To compare the FGFR4 expression in tumor tissue or adjacent normal tissue, we detected the FGFR4 mRNA level from 12 pairs of frozen samples of NSCLC with quantitative PCR. It turned out that FGFR4 mRNA in tumor tissues was significantly higher than mRNA in normal tissues (Figure 1E).

Bottom Line: Fibroblast growth factor receptor 4 (FGFR4) has been proved to be correlated with progression and prognosis in many cancers.Moreover, we evaluated the prognostic value of FGFR4 by Kaplan-Meier survival curve and Cox regression model.FGFR4 expression was significantly associated with tumor diameter (P=0.039).

View Article: PubMed Central - PubMed

Affiliation: Department of General Medicine, Linyi Hospital Affiliated to Shandong University, Linyi City, People's Republic of China.

ABSTRACT

Background: Fibroblast growth factor receptor 4 (FGFR4) has been proved to be correlated with progression and prognosis in many cancers. However, the significance of FGFR4 in non-small-cell lung cancer (NSCLC) is still not well elucidated.

Methods: In our experiment, we detected FGFR4 expression in 237 samples of NSCLC with immunohistochemistry, and further analyzed the correlation between FGFR4 and clinicopathologic features of NSCLC with chi-square test. Moreover, we evaluated the prognostic value of FGFR4 by Kaplan-Meier survival curve and Cox regression model. By regulating the expression of FGFR4 by overexpression or knockdown, we assessed the role of FGFR4 on NSCLC cell proliferation.

Results: FGFR4 expression was high in NSCLC (46.8%, 111/237). FGFR4 expression was significantly associated with tumor diameter (P=0.039). With univariate (P=0.009) and multivariate (P=0.002) analysis, FGFR4 was identified as an independent prognostic factor in NSCLC (P=0.009). Moreover, FGFR4 can promote the proliferation of NSCLC cell lines.

Conclusion: FGFR4 is an independent prognostic biomarker in NSCLC. FGFR4 can accelerate the proliferation of NSCLC cell lines, indicating FGFR4 could be a potential drug target of NSCLC.

No MeSH data available.


Related in: MedlinePlus