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Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles.

Ilovitsh T, Danan Y, Meir R, Meiri A, Zalevsky Z - Sci Rep (2015)

Bottom Line: In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection.The final image where the GNPs are spatially separated is obtained computationally.The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

View Article: PubMed Central - PubMed

Affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.

ABSTRACT
In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

No MeSH data available.


Related in: MedlinePlus

Experimental results.(a) Zoom-in on an area inside the sample that contains three spots. The image was captured under conditions of continuous illumination of the sample with the two lasers at high power of 50 mW. (b) The same area with the proposed method, where each of the spots contains two different types of GNPs.
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f7: Experimental results.(a) Zoom-in on an area inside the sample that contains three spots. The image was captured under conditions of continuous illumination of the sample with the two lasers at high power of 50 mW. (b) The same area with the proposed method, where each of the spots contains two different types of GNPs.

Mentions: In order to validate the ability of the method to detect overlapping GNPs, a reference image of the sample was taken using continuous illumination with the two lasers at high power of 50 mW. The obtained image was of all the GNPs in the sample, where areas of overlapping GNPs appear as larger spots. Figure 7(a) is a zoom-in on a 40 × 50 pixels area inside the sample that contains three spots. The same area with the proposed method is presented in Fig. 7(b), where each of the spots contain two different types of GNPs. The experimental results validated the proposed concept.


Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles.

Ilovitsh T, Danan Y, Meir R, Meiri A, Zalevsky Z - Sci Rep (2015)

Experimental results.(a) Zoom-in on an area inside the sample that contains three spots. The image was captured under conditions of continuous illumination of the sample with the two lasers at high power of 50 mW. (b) The same area with the proposed method, where each of the spots contains two different types of GNPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4447069&req=5

f7: Experimental results.(a) Zoom-in on an area inside the sample that contains three spots. The image was captured under conditions of continuous illumination of the sample with the two lasers at high power of 50 mW. (b) The same area with the proposed method, where each of the spots contains two different types of GNPs.
Mentions: In order to validate the ability of the method to detect overlapping GNPs, a reference image of the sample was taken using continuous illumination with the two lasers at high power of 50 mW. The obtained image was of all the GNPs in the sample, where areas of overlapping GNPs appear as larger spots. Figure 7(a) is a zoom-in on a 40 × 50 pixels area inside the sample that contains three spots. The same area with the proposed method is presented in Fig. 7(b), where each of the spots contain two different types of GNPs. The experimental results validated the proposed concept.

Bottom Line: In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection.The final image where the GNPs are spatially separated is obtained computationally.The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

View Article: PubMed Central - PubMed

Affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.

ABSTRACT
In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

No MeSH data available.


Related in: MedlinePlus