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Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles.

Ilovitsh T, Danan Y, Meir R, Meiri A, Zalevsky Z - Sci Rep (2015)

Bottom Line: In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection.The final image where the GNPs are spatially separated is obtained computationally.The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

View Article: PubMed Central - PubMed

Affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.

ABSTRACT
In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

No MeSH data available.


Related in: MedlinePlus

The experimental setup is made of a function generator with two outputs, that modulate a green laser at wavelength of 532 nm and a red laser at wavelength of 785 nm at two frequencies of ν01 = 13 Hz and ν02 = 25 Hz respectively.The modulated beams illuminate the sample and the scattered light is recorded as function of time using a CMOS camera.
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f4: The experimental setup is made of a function generator with two outputs, that modulate a green laser at wavelength of 532 nm and a red laser at wavelength of 785 nm at two frequencies of ν01 = 13 Hz and ν02 = 25 Hz respectively.The modulated beams illuminate the sample and the scattered light is recorded as function of time using a CMOS camera.

Mentions: The proposed method was tested using the experimental setup that is described in Fig. 4.


Cellular superresolved imaging of multiple markers using temporally flickering nanoparticles.

Ilovitsh T, Danan Y, Meir R, Meiri A, Zalevsky Z - Sci Rep (2015)

The experimental setup is made of a function generator with two outputs, that modulate a green laser at wavelength of 532 nm and a red laser at wavelength of 785 nm at two frequencies of ν01 = 13 Hz and ν02 = 25 Hz respectively.The modulated beams illuminate the sample and the scattered light is recorded as function of time using a CMOS camera.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4447069&req=5

f4: The experimental setup is made of a function generator with two outputs, that modulate a green laser at wavelength of 532 nm and a red laser at wavelength of 785 nm at two frequencies of ν01 = 13 Hz and ν02 = 25 Hz respectively.The modulated beams illuminate the sample and the scattered light is recorded as function of time using a CMOS camera.
Mentions: The proposed method was tested using the experimental setup that is described in Fig. 4.

Bottom Line: In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection.The final image where the GNPs are spatially separated is obtained computationally.The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

View Article: PubMed Central - PubMed

Affiliation: 1] Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel [2] The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.

ABSTRACT
In this paper we present a technique aimed for simultaneous detection of multiple types of gold nanoparticles (GNPs) within a biological sample, using lock-in detection. We image the sample using a number of modulated laser beams that correspond to the number of GNP species that label a given sample. The final image where the GNPs are spatially separated is obtained computationally. The proposed method enables the simultaneous superresolved imaging of different areas of interest within biological sample and also the spatial separation of GNPs at sub-diffraction distances, making it a useful tool in the study of intracellular trafficking pathways in living cells.

No MeSH data available.


Related in: MedlinePlus