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Methods for Detecting the Environmental Coccoid Form of Helicobacter pylori.

Mazaheri Assadi M, Chamanrokh P, Whitehouse CA, Huq A - Front Public Health (2015)

Bottom Line: The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid.H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such as exposure to sub-inhibitory concentrations of antimicrobial agents.We further discuss the use of these detection methods including their accuracy and efficiency.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Group, Biotechnology Department, Iranian Research Organization for Science and Technology , Tehran , Iran.

ABSTRACT
Helicobacter pylori is recognized as the most common pathogen to cause gastritis, peptic and duodenal ulcers, and gastric cancer. The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid. H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such as exposure to sub-inhibitory concentrations of antimicrobial agents. The putative infectious capability and the viability of H. pylori under environmental conditions are controversial. This disagreement is partially due to the fact of lack in detecting the coccoid form of H. pylori in the environment. Accurate and effective detection methods of H. pylori will lead to rapid treatment and disinfection, and less human health damages and reduction in health care costs. In this review, we provide a brief introduction to H. pylori environmental coccoid forms, their transmission, and detection methods. We further discuss the use of these detection methods including their accuracy and efficiency.

No MeSH data available.


Related in: MedlinePlus

Morphological appearances of Helicobacter pylori. (A) Rod-shaped (B) Coccoid form. Original magnification × 20,000 (58).
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Figure 2: Morphological appearances of Helicobacter pylori. (A) Rod-shaped (B) Coccoid form. Original magnification × 20,000 (58).

Mentions: Coccoid forms have been divided into two types, a and b, by electron microscopy although the function of the two different coccoid forms of H. pylori is unclear. One possibility is that coccoid form in general represents a degenerating state of the organism (53). Kusters et al. (54) indicated that the coccoid cells of H. pylori were the morphological manifestation of bacterial cell death, observing the transformation process by electron microscopy. However, others suggested this form to be VBNC (55). Benaissa et al. (56) asserted that coccoid H. pylori was devoid of degenerative change. Willén et al. (57) studied morphologic conversion of H. pylori from spiral (Figure 2A) to coccoid (Figure 2B) form where scanning electron microscopy and transmission electron microscopy were employed.


Methods for Detecting the Environmental Coccoid Form of Helicobacter pylori.

Mazaheri Assadi M, Chamanrokh P, Whitehouse CA, Huq A - Front Public Health (2015)

Morphological appearances of Helicobacter pylori. (A) Rod-shaped (B) Coccoid form. Original magnification × 20,000 (58).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446911&req=5

Figure 2: Morphological appearances of Helicobacter pylori. (A) Rod-shaped (B) Coccoid form. Original magnification × 20,000 (58).
Mentions: Coccoid forms have been divided into two types, a and b, by electron microscopy although the function of the two different coccoid forms of H. pylori is unclear. One possibility is that coccoid form in general represents a degenerating state of the organism (53). Kusters et al. (54) indicated that the coccoid cells of H. pylori were the morphological manifestation of bacterial cell death, observing the transformation process by electron microscopy. However, others suggested this form to be VBNC (55). Benaissa et al. (56) asserted that coccoid H. pylori was devoid of degenerative change. Willén et al. (57) studied morphologic conversion of H. pylori from spiral (Figure 2A) to coccoid (Figure 2B) form where scanning electron microscopy and transmission electron microscopy were employed.

Bottom Line: The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid.H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such as exposure to sub-inhibitory concentrations of antimicrobial agents.We further discuss the use of these detection methods including their accuracy and efficiency.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Group, Biotechnology Department, Iranian Research Organization for Science and Technology , Tehran , Iran.

ABSTRACT
Helicobacter pylori is recognized as the most common pathogen to cause gastritis, peptic and duodenal ulcers, and gastric cancer. The organisms are found in two forms: (1) spiral-shaped bacillus and (2) coccoid. H. pylori coccoid form, generally found in the environment, is the transformed form of the normal spiral-shaped bacillus after exposed to water or adverse environmental conditions such as exposure to sub-inhibitory concentrations of antimicrobial agents. The putative infectious capability and the viability of H. pylori under environmental conditions are controversial. This disagreement is partially due to the fact of lack in detecting the coccoid form of H. pylori in the environment. Accurate and effective detection methods of H. pylori will lead to rapid treatment and disinfection, and less human health damages and reduction in health care costs. In this review, we provide a brief introduction to H. pylori environmental coccoid forms, their transmission, and detection methods. We further discuss the use of these detection methods including their accuracy and efficiency.

No MeSH data available.


Related in: MedlinePlus